Abstract

A spatial shift between channels in a dual-beam raster-scan imaging system introduces a temporal separation between images from the two channels that can be much shorter than the frame rate of the system. The technique is demonstrated by measuring the velocity of erythrocytes in the retinal capillaries. We used an adaptive optics scanning laser ophthalmoscope and introduced a temporal separation between imaging channels of 4.7 ms. We imaged three subjects and measured changing capillary blood flow velocity at the pulse rate. Since the time shift between channels is easily and continuously adjustable, this method can be used to measure rapidly changing events in any raster scan system with little added complexity.

© 2016 Optical Society of America

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Supplementary Material (2)

NameDescription
» Visualization 1: AVI (1712 KB)      (a) Erythrocytes travelling through the foveal capillaries imaged with the AOSLO collecting multiply scattered light. (b) Normalized video to underline moving features.
» Visualization 2: AVI (1225 KB)      Average cell image for both channels, (a) and (b). Displacement between images (c) and velocity of the cell (d) found when averaging over three consecutive frames.

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