Abstract

We show that light sheet fluorescence microscopy with structured and pivoting illumination enables fast image acquisition and improved image quality. A one-dimensional spatial light phase modulator is used to control the illumination profile at high speed. To demonstrate the features of the system, we image fluorescent beads and biological samples, successfully obtaining optically sectioned images with higher contrast using structured illumination and with reduced shadowing effects using pivoting illumination.

© 2016 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Functional in vivo imaging using fluorescence lifetime light-sheet microscopy

Claire A. Mitchell, Simon P. Poland, James Seyforth, Jakub Nedbal, Thomas Gelot, Tahiyat Huq, Gerhard Holst, Robert D. Knight, and Simon M. Ameer-Beg
Opt. Lett. 42(7) 1269-1272 (2017)

Cellular imaging of deep organ using two-photon Bessel light-sheet nonlinear structured illumination microscopy

Ming Zhao, Han Zhang, Yu Li, Amit Ashok, Rongguang Liang, Weibin Zhou, and Leilei Peng
Biomed. Opt. Express 5(5) 1296-1308 (2014)

Structured light sheet fluorescence microscopy based on four beam interference

Ming Lei and Andreas Zumbusch
Opt. Express 18(18) 19232-19241 (2010)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (7)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription