Abstract
A biological temperature measurement method based on the fluorescence intensity ratio (FIR) was developed to reduce uncertainty. The upconversion luminescence of , Er nanocrystals was studied as a function of temperature around the physiologically relevant range of 300–330 K. We found that the green–green FIR Fe and red–green FIR () varied linearly as temperature increased. The thermometric uncertainties using the two FIRs were discussed and were determined to be almost constant at 0.6 and 0.09 K for green–green and red–green, respectively. The lower thermometric uncertainty comes from the intense signal-to-noise ratio of the measured FIRs owing to their comparable fluorescence intensities.
© 2016 Optical Society of America
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