Abstract

We report on the development of a unique lock-in-detection-free line-scan stimulated Raman scattering microscopy technique based on a linear detector with a large full well capacity controlled by a field-programmable gate array (FPGA) for near video-rate Raman imaging. With the use of parallel excitation and detection scheme, the line-scan SRS imaging at 20 frames per second can be acquired with a 5-fold lower excitation power density, compared to conventional point-scan SRS imaging. The rapid data communication between the FPGA and the linear detector allows a high line-scanning rate to boost the SRS imaging speed without the need for lock-in detection. We demonstrate this lock-in-detection-free line-scan SRS imaging technique using the 0.5 μm polystyrene and 1.0 μm poly(methyl methacrylate) beads mixed in water, as well as living gastric cancer cells.

© 2016 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Stimulated Raman scattering flow cytometry for label-free single-particle analysis

Chi Zhang, Kai-Chih Huang, Bartek Rajwa, Junjie Li, Shiqi Yang, Haonan Lin, Chien-sheng Liao, Gregory Eakins, Shihuan Kuang, Valery Patsekin, J. Paul Robinson, and Ji-Xin Cheng
Optica 4(1) 103-109 (2017)

Simultaneous dual-channel stimulated Raman scattering microscopy demultiplexed at distinct modulation frequencies

Sandro Heuke, Barbara Sarri, Xavier Audier, and Hervé Rigneault
Opt. Lett. 43(15) 3582-3585 (2018)

High-speed stimulated hyperspectral Raman imaging using rapid acousto-optic delay lines

Mohammed S. Alshaykh, Chien-Sheng Liao, Oscar E. Sandoval, Gregory Gitzinger, Nicolas Forget, Daniel E. Leaird, Ji-Xin Cheng, and Andrew M. Weiner
Opt. Lett. 42(8) 1548-1551 (2017)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (1)

NameDescription
» Visualization 1: AVI (458 KB)      SRS video imaging on 1.0 µm PMMA beads acquired during Brownian motion in water.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription