Abstract

We report on the development of a unique lock-in-detection-free line-scan stimulated Raman scattering microscopy technique based on a linear detector with a large full well capacity controlled by a field-programmable gate array (FPGA) for near video-rate Raman imaging. With the use of parallel excitation and detection scheme, the line-scan SRS imaging at 20 frames per second can be acquired with a 5-fold lower excitation power density, compared to conventional point-scan SRS imaging. The rapid data communication between the FPGA and the linear detector allows a high line-scanning rate to boost the SRS imaging speed without the need for lock-in detection. We demonstrate this lock-in-detection-free line-scan SRS imaging technique using the 0.5 μm polystyrene and 1.0 μm poly(methyl methacrylate) beads mixed in water, as well as living gastric cancer cells.

© 2016 Optical Society of America

Full Article  |  PDF Article
More Like This
Stimulated Raman scattering microscopy and spectroscopy with a rapid scanning optical delay line

Ruoyu He, Zhiping Liu, Yongkui Xu, Wei Huang, Hong Ma, and Minbiao Ji
Opt. Lett. 42(4) 659-662 (2017)

Stimulated Raman scattering microscopy by Nyquist modulation of a two-branch ultrafast fiber source

Claudius Riek, Claudius Kocher, Peyman Zirak, Christoph Kölbl, Peter Fimpel, Alfred Leitenstorfer, Andreas Zumbusch, and Daniele Brida
Opt. Lett. 41(16) 3731-3734 (2016)

Dual-phase stimulated Raman scattering microscopy for real-time two-color imaging

Ruoyu He, Yongkui Xu, Lili Zhang, Shenghong Ma, Xu Wang, Dan Ye, and Minbiao Ji
Optica 4(1) 44-47 (2017)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Supplementary Material (1)

NameDescription
Visualization 1: AVI (458 KB)      SRS video imaging on 1.0 µm PMMA beads acquired during Brownian motion in water.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription