Abstract

Multispectral tissue imaging based on optical cameras and continuous-wave tissue illumination is commonly used in medicine and biology. Surprisingly, there is a characteristic absence of a critical look at the quantities that can be uniquely characterized from optically diffuse matter by multispectral imaging. Here, we investigate the fundamental question of uniqueness in epi-illumination measurements from turbid media obtained at multiple wavelengths. By utilizing an analytical model, tissue-mimicking phantoms, and an in vivo imaging experiment we show that independent of the bands employed, spectral measurements cannot uniquely retrieve absorption and scattering coefficients. We also establish that it is, nevertheless, possible to uniquely quantify oxygen saturation and the Mie scattering power—a previously undocumented uniqueness condition.

© 2016 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Uniqueness and wavelength optimization in continuous-wave multispectral diffuse optical tomography

Alper Corlu, Turgut Durduran, Regine Choe, Martin Schweiger, Elizabeth M. C. Hillman, Simon R. Arridge, and Arjun G. Yodh
Opt. Lett. 28(23) 2339-2341 (2003)

Intraoperative measurement of bowel oxygen saturation using a multispectral imaging laparoscope

Neil T. Clancy, Shobhit Arya, Danail Stoyanov, Mohan Singh, George B. Hanna, and Daniel S. Elson
Biomed. Opt. Express 6(10) 4179-4190 (2015)

Illuminant estimation in multispectral imaging

Haris Ahmad Khan, Jean-Baptiste Thomas, Jon Yngve Hardeberg, and Olivier Laligant
J. Opt. Soc. Am. A 34(7) 1085-1098 (2017)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (2)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription