Abstract

Optical coherence tomography (OCT) and optical coherence microscopy (OCM) have demonstrated the ability to investigate cyto- and myelo-architecture in the brain. Polarization-sensitive OCT provides sensitivity to additional contrast mechanisms, specifically the birefringence of myelination and, therefore, is advantageous for investigating white matter fiber tracts. In this Letter, we developed a polarization-sensitive optical coherence microscope (PS-OCM) with a 3.5 μm axial and 1.3 μm transverse resolution to investigate fiber organization and orientation at a finer scale than previously demonstrated with PS-OCT. In a reconstructed mouse brain section, we showed that at the focal depths of 20–70 μm, the PS-OCM reliably identifies the neuronal fibers and quantifies the in-plane orientation.

© 2016 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Ex vivo and in vivo imaging of myelin fibers in mouse brain by coherent anti-Stokes Raman scattering microscopy

Yan Fu, T. Brandon Huff, Han-Wei Wang, Haifeng Wang, and Ji-Xin Cheng
Opt. Express 16(24) 19396-19409 (2008)

Spectroscopic imaging with spectral domain visible light optical coherence microscopy in Alzheimer’s disease brain samples

Antonia Lichtenegger, Danielle J. Harper, Marco Augustin, Pablo Eugui, Martina Muck, Johanna Gesperger, Christoph K. Hitzenberger, Adelheid Woehrer, and Bernhard Baumann
Biomed. Opt. Express 8(9) 4007-4025 (2017)

Noninvasive, in vivo imaging of subcortical mouse brain regions with 1.7  μm optical coherence tomography

Shau Poh Chong, Conrad W. Merkle, Dylan F. Cooke, Tingwei Zhang, Harsha Radhakrishnan, Leah Krubitzer, and Vivek J. Srinivasan
Opt. Lett. 40(21) 4911-4914 (2015)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription