Abstract

Visualizing stiffness within the local tissue environment at the cellular and subcellular level promises to provide insight into the genesis and progression of disease. In this Letter, we propose ultrahigh-resolution optical coherence elastography (UHROCE), and demonstrate 3D imaging of local axial strain of tissues undergoing compressive loading. We combine optical coherence microscopy (OCM) and phase-sensitive detection of local tissue displacement to produce strain elastograms with resolution (x,y,z) of 2×2×15μm. We demonstrate this performance on a freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography (OCE) system.

© 2015 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Ultrahigh-resolution optical coherence elastography through a micro-endoscope: towards in vivo imaging of cellular-scale mechanics

Qi Fang, Andrea Curatolo, Philip Wijesinghe, Yen Ling Yeow, Juliana Hamzah, Peter B. Noble, Karol Karnowski, David D. Sampson, Ruth Ganss, Jun Ki Kim, Woei M. Lee, and Brendan F. Kennedy
Biomed. Opt. Express 8(11) 5127-5138 (2017)

Spectroscopic optical coherence elastography

Steven G. Adie, Xing Liang, Brendan F. Kennedy, Renu John, David D. Sampson, and Stephen A. Boppart
Opt. Express 18(25) 25519-25534 (2010)

Strain estimation in phase-sensitive optical coherence elastography

Brendan F. Kennedy, Sze Howe Koh, Robert A. McLaughlin, Kelsey M. Kennedy, Peter R. T. Munro, and David D. Sampson
Biomed. Opt. Express 3(8) 1865-1879 (2012)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription