Abstract

Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program optoacoustic signal time courses that were temporally unmixed to increase the proteins’ contrast-to-noise-ratios (CNRs) in optoacoustic imaging. In this way, two variants of the RSFP Dronpa with very similar optoacoustic spectra could be readily discriminated in the presence of highly absorbing blood. Addition of temporal unmixing to multispectral optoacoustic tomography (tuMSOT) in conjunction with synthetic or genetically encoded photochromic contrast agents and customized photoswitching schedules can increase the performance of multiplexed and high-contrast molecular optoacoustic imaging.

© 2015 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Focal switching of photochromic fluorescent proteins enables multiphoton microscopy with superior image contrast

Ya-Ting Kao, Xinxin Zhu, Fang Xu, and Wei Min
Biomed. Opt. Express 3(8) 1955-1963 (2012)

Convective-diffusion-based fluorescence correlation spectroscopy for detection of a trace amount of E. coli in water

De-Kui Qing, M. Pinar Mengüç, Fred A. Payne, and Mary-Grace C. Danao
Appl. Opt. 42(16) 2987-2994 (2003)

Functional optoacoustic neuro-tomography of calcium fluxes in adult zebrafish brain in vivo

X. Luís Deán-Ben, Sven Gottschalk, Gali Sela, Shy Shoham, and Daniel Razansky
Opt. Lett. 42(5) 959-962 (2017)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription