Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Guoke Wei; Dorin Simionesie; Jan Sefcik; Jens U. Sutter; Qingjiang Xue; Jun Yu; Jinliang Wang; David J. S. Birch; Yu Chen

doi:10.1364/OL.40.005738

Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Guoke Wei, Dorin Simionesie, Jan Sefcik, Jens U. Sutter, Qingjiang Xue, Jun Yu, Jinliang Wang, David J. S. Birch, and Yu Chen

Optics Letters
Vol. 40,
Issue 24,
pp. 5738-5741
(2015)
•https://doi.org/10.1364/OL.40.005738

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Guoke Wei, Dorin Simionesie, Jan Sefcik, Jens U. Sutter, Qingjiang Xue, Jun Yu, Jinliang Wang, David J. S. Birch, and Yu Chen, "Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy," Opt. Lett. 40, 5738-5741 (2015)

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Table of Contents Category
- Spectroscopy
Optics & Photonics Topics
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The topics in this list come from the OSA Optics and Photonics Topics applied to this article.
Previously assigned OCIS codes
- Nanomaterials (160.4236)
- Lifetime-based sensing (170.3650)
- Spectroscopy, fluorescence and luminescence (170.6280)

About this Article
History
- Original Manuscript: September 7, 2015
- Manuscript Accepted: November 4, 2015
- Published: December 9, 2015
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Abstract

We demonstrate that time-resolved fluorescence spectroscopy is a powerful tool to investigate the conformation states of hairpin DNA on the surface of gold nanoparticles (AuNPs) and energy transfer processes in Au-nanobeacons. Long-range fluorescence quenching of Cy5 by AuNPs has been found to be in good agreement with electrodynamics modeling. Moreover, time-correlated single-photon counting (TCSPC) is shown to be promising for real-time monitoring of the hybridization kinetics of Au-nanobeacons, with up to 60% increase in decay time component and 300% increase in component fluorescence fraction observed. Our results also indicate the importance of the stem and spacer designs for the performance of Au-nanobeacons.

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Corrections

10 December 2015: Corrections were made to Table 1.

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Name	Sequence ( $5^{'}$ to $3^{'}$ ) ^a	Hairpin $Δ G$ (kcal/mol) ^b
AAhpCy5	Cy5-CTGACTTG GTG AAG CTA ACG TTG AG CAAGTCAG AA-SH	−6.84
hpCy5	Cy5-CCGGTG GTG AAG CTA ACG TTG AG CACCGG TTT TT-SH	−5.85
hpCy5f	Cy5-AA TTT AAATTGAACTTG GTG AAG CTA ACG TTG AG CAAGTTCAATTT TTT TTT TTT T-SH	−10.54
cDNA ^c	CTC AAC GTT AGC TTC AC

Sample	$\bar{τ} / ns$	$τ_{1} / ns$ ^a	$τ_{2} / ns$ ^a	$τ_{3} / ns$ ^a	$f_{1}$	$f_{2}$	$f_{3}$	$χ_{R}^{2}$
AAhpCy5	1.436	$1.793 \pm 0.025$	$0.923 \pm 0.056$		0.590	0.410		1.056
AuNP-AAhpCy5	0.160	$0.887 \pm 0.087$	$0.251 \pm 0.031$	$0.032 \pm 0.008$	0.091	0.229	0.680	1.558
AuNP-AAhpCy5 + cDNA	0.485	$0.886 \pm 0.044$	$0.401 \pm 0.030$	$0.092 \pm 0.043$	0.243	0.648	0.109	1.165
hpCy5	1.858	$2.116 \pm 0.022$	$1.110 \pm 0.102$		0.744	0.256		1.091
AuNP-hpCy5	0.178	$1.381 \pm 0.106$	$0.302 \pm 0.038$	$0.027 \pm 0.005$	0.083	0.141	0.776	1.275
AuNP-hpCy5 + cDNA	0.521	$1.017 \pm 0.038$	$0.412 \pm 0.030$	$0.063 \pm 0.024$	0.276	0.558	0.166	1.265
hpCy5f	1.718	$1.990 \pm 0.022$	$1.053 \pm 0.089$		0.710	0.290		1.037
AuNP-hpCy5f	0.329	$1.291 \pm 0.060$	$0.372 \pm 0.051$	$0.037 \pm 0.005$	0.190	0.163	0.647	1.350
AuNP-hpCy5f + cDNA	0.800	$1.300 \pm 0.029$	$0.569 \pm 0.060$	$0.047 \pm 0.010$	0.467	0.322	0.211	1.235

Name	Sequence ( $5^{'}$ to $3^{'}$ ) ^a	Hairpin $Δ G$ (kcal/mol) ^b
AAhpCy5	Cy5-CTGACTTG GTG AAG CTA ACG TTG AG CAAGTCAG AA-SH	−6.84
hpCy5	Cy5-CCGGTG GTG AAG CTA ACG TTG AG CACCGG TTT TT-SH	−5.85
hpCy5f	Cy5-AA TTT AAATTGAACTTG GTG AAG CTA ACG TTG AG CAAGTTCAATTT TTT TTT TTT T-SH	−10.54
cDNA ^c	CTC AAC GTT AGC TTC AC

Sample	$\bar{τ} / ns$	$τ_{1} / ns$ ^a	$τ_{2} / ns$ ^a	$τ_{3} / ns$ ^a	$f_{1}$	$f_{2}$	$f_{3}$	$χ_{R}^{2}$
AAhpCy5	1.436	$1.793 \pm 0.025$	$0.923 \pm 0.056$		0.590	0.410		1.056
AuNP-AAhpCy5	0.160	$0.887 \pm 0.087$	$0.251 \pm 0.031$	$0.032 \pm 0.008$	0.091	0.229	0.680	1.558
AuNP-AAhpCy5 + cDNA	0.485	$0.886 \pm 0.044$	$0.401 \pm 0.030$	$0.092 \pm 0.043$	0.243	0.648	0.109	1.165
hpCy5	1.858	$2.116 \pm 0.022$	$1.110 \pm 0.102$		0.744	0.256		1.091
AuNP-hpCy5	0.178	$1.381 \pm 0.106$	$0.302 \pm 0.038$	$0.027 \pm 0.005$	0.083	0.141	0.776	1.275
AuNP-hpCy5 + cDNA	0.521	$1.017 \pm 0.038$	$0.412 \pm 0.030$	$0.063 \pm 0.024$	0.276	0.558	0.166	1.265
hpCy5f	1.718	$1.990 \pm 0.022$	$1.053 \pm 0.089$		0.710	0.290		1.037
AuNP-hpCy5f	0.329	$1.291 \pm 0.060$	$0.372 \pm 0.051$	$0.037 \pm 0.005$	0.190	0.163	0.647	1.350
AuNP-hpCy5f + cDNA	0.800	$1.300 \pm 0.029$	$0.569 \pm 0.060$	$0.047 \pm 0.010$	0.467	0.322	0.211	1.235

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