Abstract

Multimodal imaging is a crucial tool when imaging biological phenomena that cannot be comprehensively captured by a single modality. Here, we introduce a theoretical framework for spatial-frequency-multiplexed microscopy via off-axis interference as a novel wide-field imaging technique that enables true simultaneous multimodal and multichannel wide-field imaging. We experimentally demonstrate this technique for single-camera, simultaneous two-channel fluorescence and one-channel quantitative-phase imaging for fluorescent microspheres and fixed cells stained for F-actin and nuclear fluorescence.

© 2015 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Structured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy

Shwetadwip Chowdhury, Will J. Eldridge, Adam Wax, and Joseph A. Izatt
Biomed. Opt. Express 8(5) 2496-2518 (2017)

Simultaneous multicolor imaging of wide-field epi-fluorescence microscopy with four-bucket detection

Kwan Seob Park, Dong Uk Kim, Jooran Lee, Geon Hee Kim, and Ki Soo Chang
Biomed. Opt. Express 7(6) 2285-2294 (2016)

Quantitative phase imaging by single-shot Hilbert–Huang phase microscopy

Maciej Trusiak, Vicente Mico, Javier Garcia, and Krzysztof Patorski
Opt. Lett. 41(18) 4344-4347 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription