Abstract

We present a method for high-throughput optofluidic particle analysis that provides both the morphological and chemical profiles of individual particles in a large heterogeneous population. This method is based on an integration of a time-stretch optical microscope with a submicrometer spatial resolution of 780 nm and a three-color fluorescence analyzer on top of an inertial-focusing microfluidic device. The integrated system can perform image- and fluorescence-based screening of particles with a high throughput of 10,000 particles/s, exceeding previously demonstrated imaging particle analyzers in terms of specificity without sacrificing throughput.

© 2015 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
High-throughput label-free image cytometry and image-based classification of live Euglena gracilis

Cheng Lei, Takuro Ito, Masashi Ugawa, Taisuke Nozawa, Osamu Iwata, Masanori Maki, Genki Okada, Hirofumi Kobayashi, Xinlei Sun, Pimsiri Tiamsak, Norimichi Tsumura, Kengo Suzuki, Dino Di Carlo, Yasuyuki Ozeki, and Keisuke Goda
Biomed. Opt. Express 7(7) 2703-2708 (2016)

High-throughput time-stretch imaging flow cytometry for multi-class classification of phytoplankton

Queenie T. K. Lai, Kelvin C. M. Lee, Anson H. L. Tang, Kenneth K. Y. Wong, Hayden K. H. So, and Kevin K. Tsia
Opt. Express 24(25) 28170-28184 (2016)

High-throughput sensing of freely flowing particles with optomechanofluidics

Kewen Han, JunHwan Kim, and Gaurav Bahl
Optica 3(6) 585-591 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription