Abstract

Holographic microscopy is an emerging biological technique that provides amplitude and quantitative phase imaging, though the contrast provided by many cell types and organelles is low, and until now no dyes were known that increased contrast. Here we show that the metallocorrole Ga(tpfc)(SO3H)2, which has a strong Soret band absorption, increases contrast in both amplitude and phase and facilitates tracking of Escherichia coli with minimal toxicity. The change in phase contrast may be calculated from the dye-absorbance spectrum using the Kramers–Kronig relations, and represents a general principle that may be applied to any dye or cell type. This enables the use of holographic microscopy for all applications in which specific labeling is desired.

© 2015 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Automated multi-parameter measurement of cardiomyocytes dynamics with digital holographic microscopy

Benjamin Rappaz, Inkyu Moon, Faliu Yi, Bahram Javidi, Pierre Marquet, and Gerardo Turcatti
Opt. Express 23(10) 13333-13347 (2015)

Proposal of three-dimensional phase contrast holographic microscopy

Naoki Fukutake and Tom D. Milster
Opt. Express 15(20) 12662-12679 (2007)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (5)

NameDescription
» Visualization 1: AVI (5281 KB)      Control cells
» Visualization 2: AVI (4825 KB)      Cells 0.2 uM dye
» Visualization 3: AVI (5442 KB)      Cells 0 uM dye
» Visualization 4: AVI (91 KB)      Control tumble
» Visualization 5: AVI (166 KB)      Dye tumble

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription