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Video-rate two-photon excited fluorescence lifetime imaging system with interleaved digitization

Ximeng Y. Dow, Shane Z. Sullivan, Ryan D. Muir, and Garth J. Simpson

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Abstract

A fast (up to video rate) two-photon excited fluorescence lifetime imaging system based on interleaved digitization is demonstrated. The system is compatible with existing beam-scanning microscopes with minor electronics and software modification. Proof-of-concept demonstrations were performed using laser dyes and biological tissue.

© 2015 Optical Society of America

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Supplementary Material (1)

NameDescription
Visualization 1: AVI (23045 KB)      Video rate fluorescence lifetime imaging of the sample translating.

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