Abstract

A fast (up to video rate) two-photon excited fluorescence lifetime imaging system based on interleaved digitization is demonstrated. The system is compatible with existing beam-scanning microscopes with minor electronics and software modification. Proof-of-concept demonstrations were performed using laser dyes and biological tissue.

© 2015 Optical Society of America

Full Article  |  PDF Article
More Like This
Absolute two-photon excitation spectra of red and far-red fluorescent probes

Mary Grace M. Velasco, Edward S. Allgeyer, Peng Yuan, Jaime Grutzendler, and Joerg Bewersdorf
Opt. Lett. 40(21) 4915-4918 (2015)

Fast bi-exponential fluorescence lifetime imaging analysis methods

David Day-Uei Li, Hongqi Yu, and Yu Chen
Opt. Lett. 40(3) 336-339 (2015)

Towards two-photon excited endogenous fluorescence lifetime imaging microendoscopy

C. H. Hage, P. Leclerc, J. Brevier, M. Fabert, C. Le Nézet, A. Kudlinski, L. Héliot, and F. Louradour
Biomed. Opt. Express 9(1) 142-156 (2018)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (1)

NameDescription
» Visualization 1: AVI (23045 KB)      Video rate fluorescence lifetime imaging of the sample translating.

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription