Video-rate two-photon excited fluorescence lifetime imaging system with interleaved digitization

Abstract

A fast (up to video rate) two-photon excited fluorescence lifetime imaging system based on interleaved digitization is demonstrated. The system is compatible with existing beam-scanning microscopes with minor electronics and software modification. Proof-of-concept demonstrations were performed using laser dyes and biological tissue.

© 2015 Optical Society of America

Absolute two-photon excitation spectra of red and far-red fluorescent probes

Mary Grace M. Velasco, Edward S. Allgeyer, Peng Yuan, Jaime Grutzendler, and Joerg Bewersdorf
Opt. Lett. 40(21) 4915-4918 (2015)

Fast bi-exponential fluorescence lifetime imaging analysis methods

David Day-Uei Li, Hongqi Yu, and Yu Chen
Opt. Lett. 40(3) 336-339 (2015)

Towards two-photon excited endogenous fluorescence lifetime imaging microendoscopy

C. H. Hage, P. Leclerc, J. Brevier, M. Fabert, C. Le Nézet, A. Kudlinski, L. Héliot, and F. Louradour
Biomed. Opt. Express 9(1) 142-156 (2018)

Intravital confocal fluorescence lifetime imaging microscopy in the second near-infrared window

Jia Yu, Rongli Zhang, Yufeng Gao, Zonghai Sheng, Min Gu, Qinchao Sun, Jiuling Liao, Ting Wu, Zhanyi Lin, Peiheng Wu, Lin Kang, Hui Li, Labao Zhang, and Wei Zheng
Opt. Lett. 45(12) 3305-3308 (2020)

Single pulse two photon fluorescence lifetime imaging (SP-FLIM) with MHz pixel rate

Matthias Eibl, Sebastian Karpf, Daniel Weng, Hubertus Hakert, Tom Pfeiffer, Jan Philip Kolb, and Robert Huber
Biomed. Opt. Express 8(7) 3132-3142 (2017)