Abstract

We developed several approaches to characterize the recently reported self-bending point spread function for 3D localization-based light microscopy. Experimentally, we generated Gaussian, astigmatic, and self-bending point spread functions. We compared the optical transfer functions, ambiguity functions, and Fisher information of these point spread functions. Our comprehensive frequency-domain analysis describes quantitative tools for the development of engineered point spread functions for 3D imaging systems.

© 2015 Optical Society of America

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