Abstract

Motion blur, which results from time-averaging an image over the camera’s exposure time, is a common problem in microscopy of moving samples. Here, we demonstrate linear motion deblurring using temporally coded illumination in an LED array microscope. By illuminating moving objects with a well-designed temporal coded sequence that varies during each single camera exposure, the resulting motion blur is invertible and can be computationally removed. This scheme is implemented in an existing LED array microscope, providing benefits of being grayscale, fast, and adaptive, which leads to high-quality deblur performance and a flexible implementation with no moving parts. The proposed method is demonstrated experimentally for fast moving targets in a microfluidic environment.

© 2015 Optical Society of America

Motion deblurring using spatiotemporal phase aperture coding

Shay Elmalem, Raja Giryes, and Emanuel Marom
Optica 7(10) 1332-1340 (2020)

Deep coded exposure: end-to-end co-optimization of flutter shutter and deblurring processing for general motion blur removal

Zhihong Zhang, Kaiming Dong, Jinli Suo, and Qionghai Dai
Photon. Res. 11(10) 1678-1686 (2023)

Time-coded aperture for x-ray imaging

Daniel Ching, Selin Aslan, Viktor Nikitin, and Doğa Gürsoy
Opt. Lett. 44(11) 2803-2806 (2019)

Multiplexed coded illumination for Fourier Ptychography with an LED array microscope

Lei Tian, Xiao Li, Kannan Ramchandran, and Laura Waller
Biomed. Opt. Express 5(7) 2376-2389 (2014)

Fluorescence imaging of flowing cells using a temporally coded excitation

Sai Siva Gorthi, Diane Schaak, and Ethan Schonbrun
Opt. Express 21(4) 5164-5170 (2013)