Abstract

Motion blur, which results from time-averaging an image over the camera’s exposure time, is a common problem in microscopy of moving samples. Here, we demonstrate linear motion deblurring using temporally coded illumination in an LED array microscope. By illuminating moving objects with a well-designed temporal coded sequence that varies during each single camera exposure, the resulting motion blur is invertible and can be computationally removed. This scheme is implemented in an existing LED array microscope, providing benefits of being grayscale, fast, and adaptive, which leads to high-quality deblur performance and a flexible implementation with no moving parts. The proposed method is demonstrated experimentally for fast moving targets in a microfluidic environment.

© 2015 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Multiplexed coded illumination for Fourier Ptychography with an LED array microscope

Lei Tian, Xiao Li, Kannan Ramchandran, and Laura Waller
Biomed. Opt. Express 5(7) 2376-2389 (2014)

Fluorescence imaging of flowing cells using a temporally coded excitation

Sai Siva Gorthi, Diane Schaak, and Ethan Schonbrun
Opt. Express 21(4) 5164-5170 (2013)

Joint nonuniform illumination estimation and deblurring for bar code signals

Jeongtae Kim and Hana Lee
Opt. Express 15(22) 14817-14837 (2007)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (6)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription