Abstract

We propose here a new three-dimensional (3D) holographic tracking method capable to track, simultaneously and in a single step, all the spatial coordinates of micro-objects. The approach is based on the enhanced correlation coefficient (ECC) maximization method but applied, for the first time to the best of our knowledge, directly on the holographic reconstructed complex wave fields. The key novelty of the proposed strategy is its ability to calculate simultaneously the 3D coordinates of cells, without decoupling the contribution of amplitude and phase. The proposed strategy is tested on living cells (i.e., NIH 3T3 mouse fibroblast) flowing into a microfluidic channel and compared with classical holographic tracking approach. Theoretical description and experimental validation of the proposed strategy are reported.

© 2014 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Recent advances in holographic 3D particle tracking

Pasquale Memmolo, Lisa Miccio, Melania Paturzo, Giuseppe Di Caprio, Giuseppe Coppola, Paolo A. Netti, and Pietro Ferraro
Adv. Opt. Photon. 7(4) 713-755 (2015)

On the holographic 3D tracking of in vitro cells characterized by a highly-morphological change

Pasquale Memmolo, Maria Iannone, Maurizio Ventre, Paolo Antonio Netti, Andrea Finizio, Melania Paturzo, and Pietro Ferraro
Opt. Express 20(27) 28485-28493 (2012)

3D tracking the Brownian motion of colloidal particles using digital holographic microscopy and joint reconstruction

Nicolas Verrier, Corinne Fournier, and Thierry Fournel
Appl. Opt. 54(16) 4996-5002 (2015)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (3)

» Media 1: AVI (4439 KB)     
» Media 2: AVI (2877 KB)     
» Media 3: AVI (1393 KB)     

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription