Abstract

We propose here a new three-dimensional (3D) holographic tracking method capable to track, simultaneously and in a single step, all the spatial coordinates of micro-objects. The approach is based on the enhanced correlation coefficient (ECC) maximization method but applied, for the first time to the best of our knowledge, directly on the holographic reconstructed complex wave fields. The key novelty of the proposed strategy is its ability to calculate simultaneously the 3D coordinates of cells, without decoupling the contribution of amplitude and phase. The proposed strategy is tested on living cells (i.e., NIH 3T3 mouse fibroblast) flowing into a microfluidic channel and compared with classical holographic tracking approach. Theoretical description and experimental validation of the proposed strategy are reported.

© 2014 Optical Society of America

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2014

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, A. Paciello, M. Ventre, P. A. Netti, and P. Ferraro, Lab Chip 14, 1129 (2014).
[CrossRef]

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[CrossRef]

2013

2012

2011

2010

2009

H. Hajjoul, S. Kocanova, I. Lassadi, K. Bystricky, and A. Bancaud, Lab Chip 9, 3054 (2009).
[CrossRef]

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

N. T. Shaked, M. T. Rinehart, and A. Wax, Opt. Lett. 34, 767 (2009).
[CrossRef]

2008

2006

Allano, D.

Alm, K.

J. Persson, A. Mölder, S. G. Pettersson, and K. Alm, Microsc. Sci. Technol. Appl. Edu. 4, 1063 (2010).

Andrés, P.

Balduzzi, D.

Bancaud, A.

H. Hajjoul, S. Kocanova, I. Lassadi, K. Bystricky, and A. Bancaud, Lab Chip 9, 3054 (2009).
[CrossRef]

Bernhardt, I.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

Bishara, W.

Boss, D.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, Nat. Photonics 7, 113 (2013).
[CrossRef]

Brunel, M.

Bystricky, K.

H. Hajjoul, S. Kocanova, I. Lassadi, K. Bystricky, and A. Bancaud, Lab Chip 9, 3054 (2009).
[CrossRef]

Calabuig, A.

Callens, N.

Cheong, F. C.

Cho, W.

Choi, Y.

Choi, Y. S.

Y. S. Choi, K. W. Seo, and S. J. Lee, Lab Chip 11, 460 (2011).
[CrossRef]

Coëtmellec, S.

Coppola, G.

Cotte, Y.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, Nat. Photonics 7, 113 (2013).
[CrossRef]

Dale, B.

Dale, R.

Dan, D.

Dasari, R. R.

Depeursinge, C.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, Nat. Photonics 7, 113 (2013).
[CrossRef]

M. F. Toy, J. Kühn, S. Richard, J. Parent, M. Egli, and C. Depeursinge, Opt. Lett. 37, 5094 (2012).
[CrossRef]

Di Caprio, G.

Dirksen, D.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

P. Langehanenberg, B. Kemper, D. Dirksen, and G. von Bally, Appl. Opt. 47, D176 (2008).
[CrossRef]

Distante, C.

Dubois, F.

Egli, M.

ElMallahi, A.

Embrione, V.

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, A. Paciello, M. Ventre, P. A. Netti, and P. Ferraro, Lab Chip 14, 1129 (2014).
[CrossRef]

Evangelidis, G. D.

G. D. Evangelidis and E. Z. Psarakis, IEEE Trans. Pattern Anal. Mach. Intell. 30, 1858 (2008).
[CrossRef]

Fang-Yen, C.

Feld, M. S.

Ferraro, P.

Finizio, A.

Fréchou, D.

Fusco, S.

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, A. Paciello, M. Ventre, P. A. Netti, and P. Ferraro, Lab Chip 14, 1129 (2014).
[CrossRef]

Galli, A.

Gao, P.

Garcia-Sucerquia, J.

Georgiev, G.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

Grasser, T.

Grier, D.

Guildenbecher, D. R.

Guo, R.

Hajjoul, H.

H. Hajjoul, S. Kocanova, I. Lassadi, K. Bystricky, and A. Bancaud, Lab Chip 9, 3054 (2009).
[CrossRef]

Hoshiba, T.

Iannone, M.

Iorio, C. S.

C. Perfetti, C. S. Iorio, A. ElMallahi, and F. Dubois, Exp. Fluids 55, 1667 (2014).
[CrossRef]

Ivanova, L.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

Javidi, B.

Jourdain, P.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, Nat. Photonics 7, 113 (2013).
[CrossRef]

Kemper, B.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

P. Langehanenberg, B. Kemper, D. Dirksen, and G. von Bally, Appl. Opt. 47, D176 (2008).
[CrossRef]

Ketelhut, S.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

Kim, M.

Kocanova, S.

H. Hajjoul, S. Kocanova, I. Lassadi, K. Bystricky, and A. Bancaud, Lab Chip 9, 3054 (2009).
[CrossRef]

Krishnatreya, B. J.

Kühn, J.

Lancis, J.

Langehanenberg, P.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

P. Langehanenberg, B. Kemper, D. Dirksen, and G. von Bally, Appl. Opt. 47, D176 (2008).
[CrossRef]

Lassadi, I.

H. Hajjoul, S. Kocanova, I. Lassadi, K. Bystricky, and A. Bancaud, Lab Chip 9, 3054 (2009).
[CrossRef]

Lebrun, D.

Lee, S. J.

Y. S. Choi, K. W. Seo, and S. J. Lee, Lab Chip 11, 460 (2011).
[CrossRef]

Lei, M.

Ma, B.

Magistretti, P.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, Nat. Photonics 7, 113 (2013).
[CrossRef]

Marquet, P.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, Nat. Photonics 7, 113 (2013).
[CrossRef]

Méès, L.

Memmolo, P.

Mendoza-Yero, O.

Menq, C.-H.

Merola, F.

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, A. Paciello, M. Ventre, P. A. Netti, and P. Ferraro, Lab Chip 14, 1129 (2014).
[CrossRef]

F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, A. Galli, R. Puglisi, D. Balduzzi, G. Coppola, P. A. Netti, and P. Ferraro, Lab Chip 13, 4512 (2013).
[CrossRef]

Miccio, L.

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, A. Paciello, M. Ventre, P. A. Netti, and P. Ferraro, Lab Chip 14, 1129 (2014).
[CrossRef]

F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, A. Galli, R. Puglisi, D. Balduzzi, G. Coppola, P. A. Netti, and P. Ferraro, Lab Chip 13, 4512 (2013).
[CrossRef]

P. Memmolo, A. Finizio, M. Paturzo, L. Miccio, and P. Ferraro, Opt. Express 19, 25833 (2011).
[CrossRef]

L. Miccio, A. Finizio, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Biomed. Opt. Express 2, 331 (2011).
[CrossRef]

Min, J.

Mölder, A.

J. Persson, A. Mölder, S. G. Pettersson, and K. Alm, Microsc. Sci. Technol. Appl. Edu. 4, 1063 (2010).

Netti, P. A.

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, A. Paciello, M. Ventre, P. A. Netti, and P. Ferraro, Lab Chip 14, 1129 (2014).
[CrossRef]

F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, A. Galli, R. Puglisi, D. Balduzzi, G. Coppola, P. A. Netti, and P. Ferraro, Lab Chip 13, 4512 (2013).
[CrossRef]

P. Memmolo, M. Iannone, M. Ventre, P. A. Netti, A. Finizio, M. Paturzo, and P. Ferraro, Opt. Express 20, 28485 (2012).
[CrossRef]

Ozcan, A.

T. W. Sua, L. Xuea, and A. Ozcan, Proc. Natl. Acad. Sci. USA 109, 16018 (2012).

W. Bishara, H. Zhu, and A. Ozcan, Opt. Express 18, 27499 (2010).
[CrossRef]

Paciello, A.

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, A. Paciello, M. Ventre, P. A. Netti, and P. Ferraro, Lab Chip 14, 1129 (2014).
[CrossRef]

Parent, J.

Paturzo, M.

Pavillon, N.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, Nat. Photonics 7, 113 (2013).
[CrossRef]

Perfetti, C.

C. Perfetti, C. S. Iorio, A. ElMallahi, and F. Dubois, Exp. Fluids 55, 1667 (2014).
[CrossRef]

Persson, J.

J. Persson, A. Mölder, S. G. Pettersson, and K. Alm, Microsc. Sci. Technol. Appl. Edu. 4, 1063 (2010).

Pettersson, S. G.

J. Persson, A. Mölder, S. G. Pettersson, and K. Alm, Microsc. Sci. Technol. Appl. Edu. 4, 1063 (2010).

Psarakis, E. Z.

G. D. Evangelidis and E. Z. Psarakis, IEEE Trans. Pattern Anal. Mach. Intell. 30, 1858 (2008).
[CrossRef]

Puglisi, R.

Restrepo, J. F.

Reu, P. L.

Richard, S.

Rinehart, M. T.

Rupp, R.

Schockaert, C.

Seo, K. W.

Y. S. Choi, K. W. Seo, and S. J. Lee, Lab Chip 11, 460 (2011).
[CrossRef]

Shaked, N. T.

Stuaffacher, H. L.

Sua, T. W.

T. W. Sua, L. Xuea, and A. Ozcan, Proc. Natl. Acad. Sci. USA 109, 16018 (2012).

Sung, Y.

Tajahuerce, E.

Toy, F.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, Nat. Photonics 7, 113 (2013).
[CrossRef]

Toy, M. F.

Ventre, M.

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, A. Paciello, M. Ventre, P. A. Netti, and P. Ferraro, Lab Chip 14, 1129 (2014).
[CrossRef]

P. Memmolo, M. Iannone, M. Ventre, P. A. Netti, A. Finizio, M. Paturzo, and P. Ferraro, Opt. Express 20, 28485 (2012).
[CrossRef]

Vollmer, A.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

von Bally, G.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

P. Langehanenberg, B. Kemper, D. Dirksen, and G. von Bally, Appl. Opt. 47, D176 (2008).
[CrossRef]

Watanabe, E.

Wax, A.

Xuea, L.

T. W. Sua, L. Xuea, and A. Ozcan, Proc. Natl. Acad. Sci. USA 109, 16018 (2012).

Yan, S.

Yao, B.

Ye, T.

Yourassowsky, C.

Zhang, Z.

Zheng, J.

Zhu, H.

Appl. Opt.

Biomed. Opt. Express

Exp. Fluids

C. Perfetti, C. S. Iorio, A. ElMallahi, and F. Dubois, Exp. Fluids 55, 1667 (2014).
[CrossRef]

IEEE Trans. Pattern Anal. Mach. Intell.

G. D. Evangelidis and E. Z. Psarakis, IEEE Trans. Pattern Anal. Mach. Intell. 30, 1858 (2008).
[CrossRef]

J. Biomed. Opt.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, J. Biomed. Opt. 14, 014018 (2009).
[CrossRef]

Lab Chip

Y. S. Choi, K. W. Seo, and S. J. Lee, Lab Chip 11, 460 (2011).
[CrossRef]

H. Hajjoul, S. Kocanova, I. Lassadi, K. Bystricky, and A. Bancaud, Lab Chip 9, 3054 (2009).
[CrossRef]

F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, A. Galli, R. Puglisi, D. Balduzzi, G. Coppola, P. A. Netti, and P. Ferraro, Lab Chip 13, 4512 (2013).
[CrossRef]

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, A. Paciello, M. Ventre, P. A. Netti, and P. Ferraro, Lab Chip 14, 1129 (2014).
[CrossRef]

Microsc. Sci. Technol. Appl. Edu.

J. Persson, A. Mölder, S. G. Pettersson, and K. Alm, Microsc. Sci. Technol. Appl. Edu. 4, 1063 (2010).

Nat. Photonics

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, Nat. Photonics 7, 113 (2013).
[CrossRef]

Opt. Express

Opt. Lett.

Proc. Natl. Acad. Sci. USA

T. W. Sua, L. Xuea, and A. Ozcan, Proc. Natl. Acad. Sci. USA 109, 16018 (2012).

Supplementary Material (3)

» Media 1: AVI (4439 KB)     
» Media 2: AVI (2877 KB)     
» Media 3: AVI (1393 KB)     

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Figures (4)

Fig. 1.
Fig. 1.

Optical arrangement of setup used in our experiment for digital holograms recording. In the red box we show a 3D vision of microchannel, while in the top right a recorded hologram, with the direction of flow, is reported.

Fig. 2.
Fig. 2.

(a), (b) Two subsequent QPMs of motile cell. In (c) we report their superimposition [the red and the green cells are the same in (a) and (b), respectively], and in the upper we report the initial values of ECC and the initial displacements. In (d) the final output of ECC maximization with the corresponding ECC value (top) and final displacements (top-right). All displacement units are expressed in micrometers (Media 1).

Fig. 3.
Fig. 3.

Frame of Media 2. (a) Tracked cell and (b) comparison of 3D positions of cell calculated by proposed method (blue line) and classical approach (cyan line).

Fig. 4.
Fig. 4.

Frame of Media 3. (a) QPM with three cells and (b) the corresponding trajectories. The blue line in (b) is the position of the cell in Fig. 3(a) that has already crossed the field of view.

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

Δpopt=argmaxECC{Ck,T(Ck+1,Δp)}pk+1=pk+Δpopt,
ECC{A,B}=Re{a¯tb¯*a¯b¯}.
TC=σ(A)/μ(A),

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