Abstract

We propose a dual-parameter detection method to realize the simultaneous optical absorption and viscoelasticity imaging based on photoacoustic lock-in measurement. Both optical absorption and viscoelasticity properties can be obtained simultaneously by analyzing the amplitude and phase of photoacoustic signals. This method is experimentally verified by imaging of gelatin phantoms with different absorption coefficients and viscoelastic coefficients. Furthermore, pilot experiments were performed on an in vivo murine EMT6 tumor from the back of a BALB/c mouse. Results demonstrate that the method can be used to measure the optical absorption and viscoelasticity of different biological tissues.

© 2014 Optical Society of America

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2013 (5)

2012 (5)

Z. J. Chen, S. H. Yang, and D. Xing, Opt. Lett. 37, 3414 (2012).
[CrossRef]

Y. Yuan, S. Yang, and D. Xing, Appl. Phys. Lett. 100, 023702 (2012).
[CrossRef]

J. Brunker and P. Beard, J. Acoust. Soc. Am. 132, 1780 (2012).
[CrossRef]

W. Song, Q. Wei, T. Liu, D. Kuai, J. M. Burke, S. Jiao, and H. F. Zhang, J. Biomed. Opt. 17, 061206 (2012).
[CrossRef]

L. V. Wang and S. Hu, Science 335, 1458 (2012).
[CrossRef]

2011 (2)

2010 (4)

S. Telenkov and A. Mandelis, Rev. Sci. Instrum. 81, 124901 (2010).
[CrossRef]

V. Ntziachristos, Nat. Methods 7, 603 (2010).
[CrossRef]

R. A. Kruger, R. B. Lam, D. R. Reinecke, S. P. DelRio, and R. P. Doyle, Med. Phys. 37, 6096 (2010).
[CrossRef]

B. Y. Hsieh, S. L. Chen, T. Ling, L. J. Guo, and P. C. Li, Opt. Lett. 35, 2892 (2010).
[CrossRef]

2009 (1)

E. I. Galanzha, E. V. Shashkov, T. Kelly, J. W. Kim, L. Yang, and V. P. Zharov, Nat. Nanotechnol. 4, 855 (2009).
[CrossRef]

2008 (1)

K. Maslov and L. V. Wang, J. Biomed. Opt. 13, 024006 (2008).
[CrossRef]

2007 (2)

H. Fang, K. Maslov, and L. V. Wang, Appl. Phys. Lett. 91, 264103 (2007).
[CrossRef]

H. Fang, K. Maslov, and L. V. Wang, Phys. Rev. Lett. 99, 184501 (2007).
[CrossRef]

2005 (2)

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Y. Fan, A. Mandelis, G. Spirou, I. A. Vitkin, and W. M. Whelan, Phys. Rev. E 72, 051908 (2005).
[CrossRef]

Beard, P.

J. Brunker and P. Beard, J. Acoust. Soc. Am. 132, 1780 (2012).
[CrossRef]

P. Beard, Interface Focus 1, 602 (2011).

Boettiger, D.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Brunker, J.

J. Brunker and P. Beard, J. Acoust. Soc. Am. 132, 1780 (2012).
[CrossRef]

Burke, J. M.

W. Song, Q. Wei, T. Liu, D. Kuai, J. M. Burke, S. Jiao, and H. F. Zhang, J. Biomed. Opt. 17, 061206 (2012).
[CrossRef]

Cai, Q. X.

Chen, J. H.

Chen, Q.

H. Qin, T. Zhou, S. H. Yang, Q. Chen, and D. Xing, Nanomedicine 20, 1 (2013).

Chen, S. L.

Chen, Z. J.

Chi, Y.

DelRio, S. P.

R. A. Kruger, R. B. Lam, D. R. Reinecke, S. P. DelRio, and R. P. Doyle, Med. Phys. 37, 6096 (2010).
[CrossRef]

Dembo, M.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Doyle, R. P.

R. A. Kruger, R. B. Lam, D. R. Reinecke, S. P. DelRio, and R. P. Doyle, Med. Phys. 37, 6096 (2010).
[CrossRef]

Fan, Y.

Y. Fan, A. Mandelis, G. Spirou, I. A. Vitkin, and W. M. Whelan, Phys. Rev. E 72, 051908 (2005).
[CrossRef]

Fang, H.

H. Fang, K. Maslov, and L. V. Wang, Appl. Phys. Lett. 91, 264103 (2007).
[CrossRef]

H. Fang, K. Maslov, and L. V. Wang, Phys. Rev. Lett. 99, 184501 (2007).
[CrossRef]

Galanzha, E. I.

E. I. Galanzha, E. V. Shashkov, T. Kelly, J. W. Kim, L. Yang, and V. P. Zharov, Nat. Nanotechnol. 4, 855 (2009).
[CrossRef]

Gao, G. D.

Gateau, J.

Gefen, A.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Guo, L. J.

Hammer, D. A.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Hsieh, B. Y.

Hu, S.

L. V. Wang and S. Hu, Science 335, 1458 (2012).
[CrossRef]

Jiang, H.

Jiao, S.

W. Song, Q. Wei, T. Liu, D. Kuai, J. M. Burke, S. Jiao, and H. F. Zhang, J. Biomed. Opt. 17, 061206 (2012).
[CrossRef]

Johnson, K. R.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Kelly, T.

E. I. Galanzha, E. V. Shashkov, T. Kelly, J. W. Kim, L. Yang, and V. P. Zharov, Nat. Nanotechnol. 4, 855 (2009).
[CrossRef]

Kim, J. W.

E. I. Galanzha, E. V. Shashkov, T. Kelly, J. W. Kim, L. Yang, and V. P. Zharov, Nat. Nanotechnol. 4, 855 (2009).
[CrossRef]

Kruger, R. A.

R. A. Kruger, R. B. Lam, D. R. Reinecke, S. P. DelRio, and R. P. Doyle, Med. Phys. 37, 6096 (2010).
[CrossRef]

Kuai, D.

W. Song, Q. Wei, T. Liu, D. Kuai, J. M. Burke, S. Jiao, and H. F. Zhang, J. Biomed. Opt. 17, 061206 (2012).
[CrossRef]

Lakins, J. N.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Lam, R. B.

R. A. Kruger, R. B. Lam, D. R. Reinecke, S. P. DelRio, and R. P. Doyle, Med. Phys. 37, 6096 (2010).
[CrossRef]

Li, P. C.

Lin, R. Q.

Ling, T.

Liu, T.

W. Song, Q. Wei, T. Liu, D. Kuai, J. M. Burke, S. Jiao, and H. F. Zhang, J. Biomed. Opt. 17, 061206 (2012).
[CrossRef]

Mandelis, A.

S. Telenkov and A. Mandelis, Rev. Sci. Instrum. 81, 124901 (2010).
[CrossRef]

Y. Fan, A. Mandelis, G. Spirou, I. A. Vitkin, and W. M. Whelan, Phys. Rev. E 72, 051908 (2005).
[CrossRef]

Margulies, S. S.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Maslov, K.

K. Maslov and L. V. Wang, J. Biomed. Opt. 13, 024006 (2008).
[CrossRef]

H. Fang, K. Maslov, and L. V. Wang, Appl. Phys. Lett. 91, 264103 (2007).
[CrossRef]

H. Fang, K. Maslov, and L. V. Wang, Phys. Rev. Lett. 99, 184501 (2007).
[CrossRef]

Meng, J.

Ntziachristos, V.

Omar, M.

Paszek, M. J.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Qin, H.

H. Qin, T. Zhou, S. H. Yang, Q. Chen, and D. Xing, Nanomedicine 20, 1 (2013).

Reinecke, D. R.

R. A. Kruger, R. B. Lam, D. R. Reinecke, S. P. DelRio, and R. P. Doyle, Med. Phys. 37, 6096 (2010).
[CrossRef]

Reinhart-King, C. A.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Rozenberg, G. I.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Shashkov, E. V.

E. I. Galanzha, E. V. Shashkov, T. Kelly, J. W. Kim, L. Yang, and V. P. Zharov, Nat. Nanotechnol. 4, 855 (2009).
[CrossRef]

Song, C.

Song, L.

Song, W.

W. Song, Q. Wei, T. Liu, D. Kuai, J. M. Burke, S. Jiao, and H. F. Zhang, J. Biomed. Opt. 17, 061206 (2012).
[CrossRef]

Spirou, G.

Y. Fan, A. Mandelis, G. Spirou, I. A. Vitkin, and W. M. Whelan, Phys. Rev. E 72, 051908 (2005).
[CrossRef]

Tang, H.

Tang, Z. L.

Telenkov, S.

S. Telenkov and A. Mandelis, Rev. Sci. Instrum. 81, 124901 (2010).
[CrossRef]

Vitkin, I. A.

Y. Fan, A. Mandelis, G. Spirou, I. A. Vitkin, and W. M. Whelan, Phys. Rev. E 72, 051908 (2005).
[CrossRef]

Wang, H. N.

Wang, L. V.

L. V. Wang and S. Hu, Science 335, 1458 (2012).
[CrossRef]

K. Maslov and L. V. Wang, J. Biomed. Opt. 13, 024006 (2008).
[CrossRef]

H. Fang, K. Maslov, and L. V. Wang, Appl. Phys. Lett. 91, 264103 (2007).
[CrossRef]

H. Fang, K. Maslov, and L. V. Wang, Phys. Rev. Lett. 99, 184501 (2007).
[CrossRef]

Weaver, V. M.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Wei, Q.

W. Song, Q. Wei, T. Liu, D. Kuai, J. M. Burke, S. Jiao, and H. F. Zhang, J. Biomed. Opt. 17, 061206 (2012).
[CrossRef]

Whelan, W. M.

Y. Fan, A. Mandelis, G. Spirou, I. A. Vitkin, and W. M. Whelan, Phys. Rev. E 72, 051908 (2005).
[CrossRef]

Wu, L. R.

Wu, Y. B.

Xi, L.

Xing, D.

H. Qin, T. Zhou, S. H. Yang, Q. Chen, and D. Xing, Nanomedicine 20, 1 (2013).

Y. Yuan, S. Yang, and D. Xing, Appl. Phys. Lett. 100, 023702 (2012).
[CrossRef]

Z. J. Chen, S. H. Yang, and D. Xing, Opt. Lett. 37, 3414 (2012).
[CrossRef]

G. D. Gao, S. H. Yang, and D. Xing, Opt. Lett. 36, 3341 (2011).
[CrossRef]

Yang, L.

E. I. Galanzha, E. V. Shashkov, T. Kelly, J. W. Kim, L. Yang, and V. P. Zharov, Nat. Nanotechnol. 4, 855 (2009).
[CrossRef]

Yang, S.

Y. Yuan, S. Yang, and D. Xing, Appl. Phys. Lett. 100, 023702 (2012).
[CrossRef]

Yang, S. H.

Yuan, Y.

Y. Yuan, S. Yang, and D. Xing, Appl. Phys. Lett. 100, 023702 (2012).
[CrossRef]

Zahir, N.

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Zhang, H. F.

W. Song, Q. Wei, T. Liu, D. Kuai, J. M. Burke, S. Jiao, and H. F. Zhang, J. Biomed. Opt. 17, 061206 (2012).
[CrossRef]

Zharov, V. P.

E. I. Galanzha, E. V. Shashkov, T. Kelly, J. W. Kim, L. Yang, and V. P. Zharov, Nat. Nanotechnol. 4, 855 (2009).
[CrossRef]

Zheng, H. R.

Zhou, T.

H. Qin, T. Zhou, S. H. Yang, Q. Chen, and D. Xing, Nanomedicine 20, 1 (2013).

Appl. Phys. Lett. (2)

Y. Yuan, S. Yang, and D. Xing, Appl. Phys. Lett. 100, 023702 (2012).
[CrossRef]

H. Fang, K. Maslov, and L. V. Wang, Appl. Phys. Lett. 91, 264103 (2007).
[CrossRef]

Cancer Cells (1)

M. J. Paszek, N. Zahir, K. R. Johnson, J. N. Lakins, G. I. Rozenberg, A. Gefen, C. A. Reinhart-King, S. S. Margulies, M. Dembo, D. Boettiger, D. A. Hammer, and V. M. Weaver, Cancer Cells 8, 241 (2005).

Interface Focus (1)

P. Beard, Interface Focus 1, 602 (2011).

J. Acoust. Soc. Am. (1)

J. Brunker and P. Beard, J. Acoust. Soc. Am. 132, 1780 (2012).
[CrossRef]

J. Biomed. Opt. (2)

W. Song, Q. Wei, T. Liu, D. Kuai, J. M. Burke, S. Jiao, and H. F. Zhang, J. Biomed. Opt. 17, 061206 (2012).
[CrossRef]

K. Maslov and L. V. Wang, J. Biomed. Opt. 13, 024006 (2008).
[CrossRef]

Med. Phys. (1)

R. A. Kruger, R. B. Lam, D. R. Reinecke, S. P. DelRio, and R. P. Doyle, Med. Phys. 37, 6096 (2010).
[CrossRef]

Nanomedicine (1)

H. Qin, T. Zhou, S. H. Yang, Q. Chen, and D. Xing, Nanomedicine 20, 1 (2013).

Nat. Methods (1)

V. Ntziachristos, Nat. Methods 7, 603 (2010).
[CrossRef]

Nat. Nanotechnol. (1)

E. I. Galanzha, E. V. Shashkov, T. Kelly, J. W. Kim, L. Yang, and V. P. Zharov, Nat. Nanotechnol. 4, 855 (2009).
[CrossRef]

Opt. Express (1)

Opt. Lett. (6)

Phys. Rev. E (1)

Y. Fan, A. Mandelis, G. Spirou, I. A. Vitkin, and W. M. Whelan, Phys. Rev. E 72, 051908 (2005).
[CrossRef]

Phys. Rev. Lett. (1)

H. Fang, K. Maslov, and L. V. Wang, Phys. Rev. Lett. 99, 184501 (2007).
[CrossRef]

Rev. Sci. Instrum. (1)

S. Telenkov and A. Mandelis, Rev. Sci. Instrum. 81, 124901 (2010).
[CrossRef]

Science (1)

L. V. Wang and S. Hu, Science 335, 1458 (2012).
[CrossRef]

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Figures (5)

Fig. 1.
Fig. 1.

(a) Principle for simultaneous optical absorption and viscoelasticity imaging. (b) Schematic setup of the dual-parameter imaging method.

Fig. 2.
Fig. 2.

Detection process of amplitude and phase of PA signal based on the lock-in measurement.

Fig. 3.
Fig. 3.

(a) Ac components of “X” and “Y” signals and (b) PA amplitude “R” and phase delay “δ” detected from the gelatin phantom.

Fig. 4.
Fig. 4.

(a) Photograph of the imaging samples. (b) Optial absorption image and (c) viscoelasticity image of the tissue-mimicking phantoms. (d) The averaged amplitude and phase delay of the PA signal from each phantom.

Fig. 5.
Fig. 5.

(a) Optical absorption image and (b) viscoelasticity image of the tumor. (c) Photograph of the tumor and the dashed frame is the scanning area. (d) The averaged amplitude and phase delay of the PA signal from the marked tumor region compared to the normal tissue. (e), (f) Hematoxylin-eosin (HE) stain of the normal tissue and tumor tissue.

Equations (6)

Equations on this page are rendered with MathJax. Learn more.

I=1/2I0[1+cos(ωt),
ε(t)=εAei(ωt+δ),
δ=arctanηω/E,
E(t)=E1E2cos(βα)2E1E2cos(2ωt+β+α)2.
R=X2+Y2,
δ=tan1Y/X.

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