Abstract

Total Internal Reflection Digital Holographic Microscopy (TIRDHM) is recognized to be a powerful tool for retrieving quantitative phase images of cell–substrate interfaces, adhesions, and tissue structures close to the prism surface. In this Letter, we develop an improved TIRDHM system, taking advantage of a refractive index mismatch between the prism and the sample substrate, to allow phase-shifting DH with just a single-beam interferometric configuration. Instead of the traditional off-axis method, phase-shift method is used to retrieve amplitude and phase images in coherent light and TIR modality. Essentially, the substrate–prism interface acts like a beam splitter generating a reference beam, where the phase-shift dependence on the incident angle is exploited in this common-path configuration. With the aim to demonstrate the technique’s validity, some experiments are performed to establish the advantage of this compact and simple configuration, in which the reference arm in the setup is avoided.

© 2014 Optical Society of America

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[CrossRef]

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[CrossRef]

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

D. Wang, L. Ding, W. Zhang, Z. Luo, H. Ou, E. Zhang, and X. Yu, Meas. Sci. Technol. 23, 065701 (2012).
[CrossRef]

2011 (1)

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, Lab Chip 11, 1276 (2011).
[CrossRef]

2010 (7)

2009 (1)

2008 (4)

J. Lee, T. Chou, and H. Shih, Opt. Lett. 33, 434 (2008).
[CrossRef]

W. M. Ash and M. K. Kim, Opt. Express 16, 9811 (2008).
[CrossRef]

G. Popescu, Methods Cell Biol. 90, 87 (2008).
[CrossRef]

M. Hÿtch, F. Houdellier, F. Hüe, and E. Snoeck, Nature 453, 1086 (2008).
[CrossRef]

2007 (3)

2006 (1)

2005 (2)

2002 (3)

L. G. Alexopoulos, G. R. Erickson, and F. Guilak, J. Microsc. 205, 125 (2002).
[CrossRef]

U. Schnars and W. Jüptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

I. Yamaguchi, T. Matsumura, and J.-I. Kato, Opt. Lett. 27, 1108 (2002).
[CrossRef]

2001 (1)

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

1999 (1)

1998 (1)

1988 (1)

B. Rothenhäusler and W. Knoll, Nature 332, 615 (1988).
[CrossRef]

1981 (1)

D. Axelrod, J. Cell Biol. 89, 141 (1981).
[CrossRef]

Alexopoulos, L. G.

L. G. Alexopoulos, G. R. Erickson, and F. Guilak, J. Microsc. 205, 125 (2002).
[CrossRef]

Ash, W. M.

Axelrod, D.

D. Axelrod, J. Cell Biol. 89, 141 (1981).
[CrossRef]

Badizadegan, K.

Balduzzi, D.

V. Bianco, M. Paturzo, A. Finizio, D. Balduzzi, R. Puglisi, A. Galli, and P. Ferraro, Opt. Lett. 37, 4212 (2012).
[CrossRef]

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

Barada, D.

Barty, A.

Bevilacqua, F.

Bianco, V.

Bishara, W.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, Lab Chip 11, 1276 (2011).
[CrossRef]

W. Bishara, H. Zhu, and A. Ozcan, Opt. Express 18, 27499 (2010).
[CrossRef]

Brown, W. J.

Castro, A.

Chalut, K. J.

Chang, N.-S.

Chen, S. J.

Chen, S.-J.

Chiu, K.-C.

Chou, T.

Chou, T. K.

J.-Y. Lee, H.-C. Shih, C.-T. Hong, and T. K. Chou, Opt. Commun. 276, 283 (2007).
[CrossRef]

Cuche, E.

Dasari, R. R.

Depeursinge, C.

N. Pavillon, J. Kühn, C. Moratal, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, PLoS One 7, e30912 (2012).
[CrossRef]

E. Cuche, F. Bevilacqua, and C. Depeursinge, Opt. Lett. 24, 291 (1999).
[CrossRef]

Ding, L.

D. Wang, L. Ding, W. Zhang, Z. Luo, H. Ou, E. Zhang, and X. Yu, Meas. Sci. Technol. 23, 065701 (2012).
[CrossRef]

Erickson, G. R.

L. G. Alexopoulos, G. R. Erickson, and F. Guilak, J. Microsc. 205, 125 (2002).
[CrossRef]

Feld, M. S.

Ferraro, P.

Finizio, A.

Frauel, Y.

Galli, A.

V. Bianco, M. Paturzo, A. Finizio, D. Balduzzi, R. Puglisi, A. Galli, and P. Ferraro, Opt. Lett. 37, 4212 (2012).
[CrossRef]

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

Guilak, F.

L. G. Alexopoulos, G. R. Erickson, and F. Guilak, J. Microsc. 205, 125 (2002).
[CrossRef]

He, R.-Y.

Hong, C.-T.

J.-Y. Lee, H.-C. Shih, C.-T. Hong, and T. K. Chou, Opt. Commun. 276, 283 (2007).
[CrossRef]

Houdellier, F.

M. Hÿtch, F. Houdellier, F. Hüe, and E. Snoeck, Nature 453, 1086 (2008).
[CrossRef]

Hu, C.

C. Hu, J. Zhong, and J. Weng, J. Biomed. Opt. 15, 056015 (2010).
[CrossRef]

Hüe, F.

M. Hÿtch, F. Houdellier, F. Hüe, and E. Snoeck, Nature 453, 1086 (2008).
[CrossRef]

Hÿtch, M.

M. Hÿtch, F. Houdellier, F. Hüe, and E. Snoeck, Nature 453, 1086 (2008).
[CrossRef]

Ikeda, T.

Isikman, S. O.

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, Lab Chip 10, 1787 (2010).
[CrossRef]

Javidi, B.

Jericho, M. H.

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Jourdain, P.

N. Pavillon, J. Kühn, C. Moratal, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, PLoS One 7, e30912 (2012).
[CrossRef]

Jüptner, W.

U. Schnars and W. Jüptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

Kato, J.-I.

Kemper, B.

B. Kemper, in Handbook of Coherent-Domain Optical Methods, V. V. Tuchin, ed. (Springer, 2013), pp. 215–257.

Kiire, T.

Kikuchi, Y.

Kim, M.

Kim, M. K.

Knoll, W.

B. Rothenhäusler and W. Knoll, Nature 332, 615 (1988).
[CrossRef]

Kreuzer, H. J.

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Krzewina, L.

Kühn, J.

N. Pavillon, J. Kühn, C. Moratal, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, PLoS One 7, e30912 (2012).
[CrossRef]

Lee, J.

Lee, J.-Y.

J.-Y. Lee, H.-C. Shih, C.-T. Hong, and T. K. Chou, Opt. Commun. 276, 283 (2007).
[CrossRef]

Lin, C.-Y.

Lo, C.-M.

Luckhart, S.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, Lab Chip 11, 1276 (2011).
[CrossRef]

Lue, N.

Luo, Z.

D. Wang, L. Ding, W. Zhang, Z. Luo, H. Ou, E. Zhang, and X. Yu, Meas. Sci. Technol. 23, 065701 (2012).
[CrossRef]

Magistretti, P. J.

N. Pavillon, J. Kühn, C. Moratal, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, PLoS One 7, e30912 (2012).
[CrossRef]

Mann, C.

Marquet, P.

N. Pavillon, J. Kühn, C. Moratal, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, PLoS One 7, e30912 (2012).
[CrossRef]

Matrecano, M.

Matsumura, T.

Meinertzhagen, I. A.

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Memmolo, P.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

M. Paturzo, P. Memmolo, A. Finizio, R. Näsänen, T. J. Naughton, and P. Ferraro, Opt. Express 18, 8806 (2010).
[CrossRef]

Moratal, C.

N. Pavillon, J. Kühn, C. Moratal, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, PLoS One 7, e30912 (2012).
[CrossRef]

Mudanyali, O.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, Lab Chip 11, 1276 (2011).
[CrossRef]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, Lab Chip 10, 1787 (2010).
[CrossRef]

Näsänen, R.

Naughton, T. J.

Nugent, K. A.

Ou, H.

D. Wang, L. Ding, W. Zhang, Z. Luo, H. Ou, E. Zhang, and X. Yu, Meas. Sci. Technol. 23, 065701 (2012).
[CrossRef]

Ozcan, A.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, Lab Chip 11, 1276 (2011).
[CrossRef]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, Lab Chip 10, 1787 (2010).
[CrossRef]

W. Bishara, H. Zhu, and A. Ozcan, Opt. Express 18, 27499 (2010).
[CrossRef]

Oztoprak, C.

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, Lab Chip 10, 1787 (2010).
[CrossRef]

Paganin, D.

Paturzo, M.

Pavillon, N.

N. Pavillon, J. Kühn, C. Moratal, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, PLoS One 7, e30912 (2012).
[CrossRef]

Popescu, G.

Puglisi, R.

V. Bianco, M. Paturzo, A. Finizio, D. Balduzzi, R. Puglisi, A. Galli, and P. Ferraro, Opt. Lett. 37, 4212 (2012).
[CrossRef]

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

Roberts, A.

Rothenhäusler, B.

B. Rothenhäusler and W. Knoll, Nature 332, 615 (1988).
[CrossRef]

Schnars, U.

U. Schnars and W. Jüptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

Sencan, I.

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, Lab Chip 10, 1787 (2010).
[CrossRef]

Shih, H.

Shih, H.-C.

J.-Y. Lee, H.-C. Shih, C.-T. Hong, and T. K. Chou, Opt. Commun. 276, 283 (2007).
[CrossRef]

Sikora, U.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, Lab Chip 11, 1276 (2011).
[CrossRef]

Snoeck, E.

M. Hÿtch, F. Houdellier, F. Hüe, and E. Snoeck, Nature 453, 1086 (2008).
[CrossRef]

Su, T.-W.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, Lab Chip 11, 1276 (2011).
[CrossRef]

Su, Y. D.

Su, Y.-D.

Tseng, D.

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, Lab Chip 10, 1787 (2010).
[CrossRef]

Wang, D.

D. Wang, L. Ding, W. Zhang, Z. Luo, H. Ou, E. Zhang, and X. Yu, Meas. Sci. Technol. 23, 065701 (2012).
[CrossRef]

Wax, A.

Weng, J.

C. Hu, J. Zhong, and J. Weng, J. Biomed. Opt. 15, 056015 (2010).
[CrossRef]

Wu, H.-L.

Xu, W.

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Yaglidere, O.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, Lab Chip 11, 1276 (2011).
[CrossRef]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, Lab Chip 10, 1787 (2010).
[CrossRef]

Yamaguchi, I.

Yatagai, T.

Yeh, T. L.

Yu, L.

Yu, X.

D. Wang, L. Ding, W. Zhang, Z. Luo, H. Ou, E. Zhang, and X. Yu, Meas. Sci. Technol. 23, 065701 (2012).
[CrossRef]

Zhang, E.

D. Wang, L. Ding, W. Zhang, Z. Luo, H. Ou, E. Zhang, and X. Yu, Meas. Sci. Technol. 23, 065701 (2012).
[CrossRef]

Zhang, W.

D. Wang, L. Ding, W. Zhang, Z. Luo, H. Ou, E. Zhang, and X. Yu, Meas. Sci. Technol. 23, 065701 (2012).
[CrossRef]

Zhong, J.

C. Hu, J. Zhong, and J. Weng, J. Biomed. Opt. 15, 056015 (2010).
[CrossRef]

Zhu, H.

Appl. Opt. (1)

J. Biomed. Opt. (1)

C. Hu, J. Zhong, and J. Weng, J. Biomed. Opt. 15, 056015 (2010).
[CrossRef]

J. Cell Biol. (1)

D. Axelrod, J. Cell Biol. 89, 141 (1981).
[CrossRef]

J. Microsc. (1)

L. G. Alexopoulos, G. R. Erickson, and F. Guilak, J. Microsc. 205, 125 (2002).
[CrossRef]

Lab Chip (3)

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, Lab Chip 10, 1787 (2010).
[CrossRef]

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, Lab Chip 11, 1276 (2011).
[CrossRef]

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab Chip 12, 3073 (2012).
[CrossRef]

Meas. Sci. Technol. (2)

U. Schnars and W. Jüptner, Meas. Sci. Technol. 13, R85 (2002).
[CrossRef]

D. Wang, L. Ding, W. Zhang, Z. Luo, H. Ou, E. Zhang, and X. Yu, Meas. Sci. Technol. 23, 065701 (2012).
[CrossRef]

Methods Cell Biol. (1)

G. Popescu, Methods Cell Biol. 90, 87 (2008).
[CrossRef]

Nature (2)

M. Hÿtch, F. Houdellier, F. Hüe, and E. Snoeck, Nature 453, 1086 (2008).
[CrossRef]

B. Rothenhäusler and W. Knoll, Nature 332, 615 (1988).
[CrossRef]

Opt. Commun. (1)

J.-Y. Lee, H.-C. Shih, C.-T. Hong, and T. K. Chou, Opt. Commun. 276, 283 (2007).
[CrossRef]

Opt. Express (7)

Opt. Lett. (9)

PLoS One (1)

N. Pavillon, J. Kühn, C. Moratal, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, PLoS One 7, e30912 (2012).
[CrossRef]

Proc. Natl. Acad. Sci. USA (1)

W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

SPIE Rev. (1)

M. Kim, SPIE Rev. 1, 018005 (2010).

Other (1)

B. Kemper, in Handbook of Coherent-Domain Optical Methods, V. V. Tuchin, ed. (Springer, 2013), pp. 215–257.

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Figures (6)

Fig. 1.
Fig. 1.

Schematic of TIRDHM setup in off-axis configuration. BS, beam splitters; M, mirrors; MO, microscope objective; BE, beam expander.

Fig. 2.
Fig. 2.

(a) Apparatus of TIRDHM system by refractive index mismatch and (b) schematic of TIRDHM setup in on-axis configuration.

Fig. 3.
Fig. 3.

(a) Four π/2 phase-shift points chosen for on-axis reconstruction. (b)–(e) Intensity images corresponding to π/2 phase-shift.

Fig. 4.
Fig. 4.

(a) Amplitude, (b) phase, and (c) pseudo-3D phase image reconstruction by proposed on-axis TIRDHM.

Fig. 5.
Fig. 5.

(a) Reconstructed Quantitative Phase Map of onion tissue cells and (b) also in pseudo 3D.

Fig. 6.
Fig. 6.

(a) and (b) Amplitude and phase reconstruction by off-axis method, respectively. (c) and (d) Amplitude and phase reconstruction by on-axis configuration, respectively.

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

Δφ=4πdnr2ni2sin2(θi)λ,
ϕ(x,y)=tan1I(x,y;3π/2)I(x,y;π/2)I(x,y;0)I(x,y;π),
O(x,y)=I(x,y;0)I(x,y;π)4cos(ϕ(x,y)).

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