Abstract

Spectral imaging is a powerful tool for a wide variety of applications; however, low imaging rates and signal-to-noise ratios (SNRs) often limit its use for many biomedical applications. Here, we present a technique for spectral imaging using a unique two-dimensional illumination pattern having spectral dispersion in one axis. The method, which is called spectrally dispersed illumination spectral imaging (SDISI), allows high-speed, high-resolution acquisition of spectral data from specimens that often cannot tolerate high illumination intensities or require fast imaging for avoiding motion artifacts. The technique is demonstrated by capturing spectral data cubes of the finger of a human volunteer using short exposure durations and a high (33.5 dB) SNR.

© 2014 Optical Society of America

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2012 (1)

2011 (1)

2010 (1)

S. Horne, D. Smith, M. Besen, M. Parlow, D. Stolyarov, H. Zhu, and W. Holber, Proc. SPIE 7680, 76800L (2010).
[CrossRef]

2006 (2)

D. Yelin, I. Rizvi, W. M. White, J. T. Motz, T. Hasan, B. E. Bouma, and G. J. Tearney, Nature 443, 765 (2006).
[CrossRef]

J. M. Dudley, G. R. Genty, and S. P. Coen, Rev. Mod. Phys. 78, 1135 (2006).
[CrossRef]

2004 (2)

X. Gao, Y. Cui, R. M. Levenson, L. W. K. Chung, and S. Nie, Nat. Biotechnol. 22, 969 (2004).
[CrossRef]

M. B. Sinclair, J. A. Timlin, D. M. Haaland, and M. Werner-Washburne, Appl. Opt. 43, 2079 (2004).
[CrossRef]

2001 (1)

D. L. Farkas and D. Becker, Pigment Cell Res. 14, 2 (2001).
[CrossRef]

1997 (1)

R. D. Shonat, E. S. Wachman, W.-H. Niu, A. P. Koretsky, and D. L. Farkas, Biophys. J. 73, 1223 (1997).
[CrossRef]

1996 (2)

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Z. Malik, D. Cabib, R. A. Buckwald, A. Talmi, Y. Garini, and S. G. Lipson, J. Microsc. 182, 133 (1996).
[CrossRef]

1991 (2)

P. J. Miller, Metrologia 28, 145 (1991).
[CrossRef]

W. G. Zijlstra, A. Buursma, and W. P. Meeuwsen-van der Roest, Clin. Chem. 37, 1633 (1991).

1975 (1)

Abramov, A.

Bar-Am, I.

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Becker, D.

D. L. Farkas and D. Becker, Pigment Cell Res. 14, 2 (2001).
[CrossRef]

Besen, M.

S. Horne, D. Smith, M. Besen, M. Parlow, D. Stolyarov, H. Zhu, and W. Holber, Proc. SPIE 7680, 76800L (2010).
[CrossRef]

Bouma, B. E.

D. Yelin, I. Rizvi, W. M. White, J. T. Motz, T. Hasan, B. E. Bouma, and G. J. Tearney, Nature 443, 765 (2006).
[CrossRef]

Buckwald, R. A.

Z. Malik, D. Cabib, R. A. Buckwald, A. Talmi, Y. Garini, and S. G. Lipson, J. Microsc. 182, 133 (1996).
[CrossRef]

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Buursma, A.

W. G. Zijlstra, A. Buursma, and W. P. Meeuwsen-van der Roest, Clin. Chem. 37, 1633 (1991).

Cabib, D.

Z. Malik, D. Cabib, R. A. Buckwald, A. Talmi, Y. Garini, and S. G. Lipson, J. Microsc. 182, 133 (1996).
[CrossRef]

Chung, L. W. K.

X. Gao, Y. Cui, R. M. Levenson, L. W. K. Chung, and S. Nie, Nat. Biotechnol. 22, 969 (2004).
[CrossRef]

Coen, S. P.

J. M. Dudley, G. R. Genty, and S. P. Coen, Rev. Mod. Phys. 78, 1135 (2006).
[CrossRef]

Cui, Y.

X. Gao, Y. Cui, R. M. Levenson, L. W. K. Chung, and S. Nie, Nat. Biotechnol. 22, 969 (2004).
[CrossRef]

du Manoir, S.

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Dudley, J. M.

J. M. Dudley, G. R. Genty, and S. P. Coen, Rev. Mod. Phys. 78, 1135 (2006).
[CrossRef]

Engel, G.

Farkas, D. L.

D. L. Farkas and D. Becker, Pigment Cell Res. 14, 2 (2001).
[CrossRef]

R. D. Shonat, E. S. Wachman, W.-H. Niu, A. P. Koretsky, and D. L. Farkas, Biophys. J. 73, 1223 (1997).
[CrossRef]

Gao, X.

X. Gao, Y. Cui, R. M. Levenson, L. W. K. Chung, and S. Nie, Nat. Biotechnol. 22, 969 (2004).
[CrossRef]

Garini, Y.

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Z. Malik, D. Cabib, R. A. Buckwald, A. Talmi, Y. Garini, and S. G. Lipson, J. Microsc. 182, 133 (1996).
[CrossRef]

Genish, H.

Genty, G. R.

J. M. Dudley, G. R. Genty, and S. P. Coen, Rev. Mod. Phys. 78, 1135 (2006).
[CrossRef]

Haaland, D. M.

Habib, D.

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Hasan, T.

D. Yelin, I. Rizvi, W. M. White, J. T. Motz, T. Hasan, B. E. Bouma, and G. J. Tearney, Nature 443, 765 (2006).
[CrossRef]

Holber, W.

S. Horne, D. Smith, M. Besen, M. Parlow, D. Stolyarov, H. Zhu, and W. Holber, Proc. SPIE 7680, 76800L (2010).
[CrossRef]

Horne, S.

S. Horne, D. Smith, M. Besen, M. Parlow, D. Stolyarov, H. Zhu, and W. Holber, Proc. SPIE 7680, 76800L (2010).
[CrossRef]

Katzir, N.

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Koretsky, A. P.

R. D. Shonat, E. S. Wachman, W.-H. Niu, A. P. Koretsky, and D. L. Farkas, Biophys. J. 73, 1223 (1997).
[CrossRef]

Lavi, M.

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Levenson, R. M.

X. Gao, Y. Cui, R. M. Levenson, L. W. K. Chung, and S. Nie, Nat. Biotechnol. 22, 969 (2004).
[CrossRef]

Lipson, S. G.

Z. Malik, D. Cabib, R. A. Buckwald, A. Talmi, Y. Garini, and S. G. Lipson, J. Microsc. 182, 133 (1996).
[CrossRef]

Macville, M.

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Malik, Z.

Z. Malik, D. Cabib, R. A. Buckwald, A. Talmi, Y. Garini, and S. G. Lipson, J. Microsc. 182, 133 (1996).
[CrossRef]

Meeuwsen-van der Roest, W. P.

W. G. Zijlstra, A. Buursma, and W. P. Meeuwsen-van der Roest, Clin. Chem. 37, 1633 (1991).

Miller, P. J.

P. J. Miller, Metrologia 28, 145 (1991).
[CrossRef]

Minai, L.

Motz, J. T.

D. Yelin, I. Rizvi, W. M. White, J. T. Motz, T. Hasan, B. E. Bouma, and G. J. Tearney, Nature 443, 765 (2006).
[CrossRef]

Nie, S.

X. Gao, Y. Cui, R. M. Levenson, L. W. K. Chung, and S. Nie, Nat. Biotechnol. 22, 969 (2004).
[CrossRef]

Niu, W.-H.

R. D. Shonat, E. S. Wachman, W.-H. Niu, A. P. Koretsky, and D. L. Farkas, Biophys. J. 73, 1223 (1997).
[CrossRef]

Parlow, M.

S. Horne, D. Smith, M. Besen, M. Parlow, D. Stolyarov, H. Zhu, and W. Holber, Proc. SPIE 7680, 76800L (2010).
[CrossRef]

Ried, T.

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Rizvi, I.

D. Yelin, I. Rizvi, W. M. White, J. T. Motz, T. Hasan, B. E. Bouma, and G. J. Tearney, Nature 443, 765 (2006).
[CrossRef]

Rosenbluh, M.

Scherock, E.

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Shonat, R. D.

R. D. Shonat, E. S. Wachman, W.-H. Niu, A. P. Koretsky, and D. L. Farkas, Biophys. J. 73, 1223 (1997).
[CrossRef]

Sinclair, M. B.

Smith, D.

S. Horne, D. Smith, M. Besen, M. Parlow, D. Stolyarov, H. Zhu, and W. Holber, Proc. SPIE 7680, 76800L (2010).
[CrossRef]

Stolyarov, D.

S. Horne, D. Smith, M. Besen, M. Parlow, D. Stolyarov, H. Zhu, and W. Holber, Proc. SPIE 7680, 76800L (2010).
[CrossRef]

Talmi, A.

Z. Malik, D. Cabib, R. A. Buckwald, A. Talmi, Y. Garini, and S. G. Lipson, J. Microsc. 182, 133 (1996).
[CrossRef]

Tearney, G. J.

D. Yelin, I. Rizvi, W. M. White, J. T. Motz, T. Hasan, B. E. Bouma, and G. J. Tearney, Nature 443, 765 (2006).
[CrossRef]

Timlin, J. A.

Wachman, E. S.

R. D. Shonat, E. S. Wachman, W.-H. Niu, A. P. Koretsky, and D. L. Farkas, Biophys. J. 73, 1223 (1997).
[CrossRef]

Werner-Washburne, M.

White, W. M.

D. Yelin, I. Rizvi, W. M. White, J. T. Motz, T. Hasan, B. E. Bouma, and G. J. Tearney, Nature 443, 765 (2006).
[CrossRef]

Wine, D.

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Wyatt, C. L.

Yelin, D.

Zhu, H.

S. Horne, D. Smith, M. Besen, M. Parlow, D. Stolyarov, H. Zhu, and W. Holber, Proc. SPIE 7680, 76800L (2010).
[CrossRef]

Zijlstra, W. G.

W. G. Zijlstra, A. Buursma, and W. P. Meeuwsen-van der Roest, Clin. Chem. 37, 1633 (1991).

Appl. Opt. (2)

Bioimaging (1)

Y. Garini, M. Macville, S. du Manoir, R. A. Buckwald, M. Lavi, N. Katzir, D. Wine, I. Bar-Am, E. Scherock, D. Habib, and T. Ried, Bioimaging 4, 65 (1996).
[CrossRef]

Biomed. Opt. Express (1)

Biophys. J. (1)

R. D. Shonat, E. S. Wachman, W.-H. Niu, A. P. Koretsky, and D. L. Farkas, Biophys. J. 73, 1223 (1997).
[CrossRef]

Clin. Chem. (1)

W. G. Zijlstra, A. Buursma, and W. P. Meeuwsen-van der Roest, Clin. Chem. 37, 1633 (1991).

J. Microsc. (1)

Z. Malik, D. Cabib, R. A. Buckwald, A. Talmi, Y. Garini, and S. G. Lipson, J. Microsc. 182, 133 (1996).
[CrossRef]

Metrologia (1)

P. J. Miller, Metrologia 28, 145 (1991).
[CrossRef]

Nat. Biotechnol. (1)

X. Gao, Y. Cui, R. M. Levenson, L. W. K. Chung, and S. Nie, Nat. Biotechnol. 22, 969 (2004).
[CrossRef]

Nature (1)

D. Yelin, I. Rizvi, W. M. White, J. T. Motz, T. Hasan, B. E. Bouma, and G. J. Tearney, Nature 443, 765 (2006).
[CrossRef]

Opt. Express (1)

Pigment Cell Res. (1)

D. L. Farkas and D. Becker, Pigment Cell Res. 14, 2 (2001).
[CrossRef]

Proc. SPIE (1)

S. Horne, D. Smith, M. Besen, M. Parlow, D. Stolyarov, H. Zhu, and W. Holber, Proc. SPIE 7680, 76800L (2010).
[CrossRef]

Rev. Mod. Phys. (1)

J. M. Dudley, G. R. Genty, and S. P. Coen, Rev. Mod. Phys. 78, 1135 (2006).
[CrossRef]

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Figures (4)

Fig. 1.
Fig. 1.

(a) Optical setup of SDISI. Top inset: color photograph of the illumination pattern. Bottom left inset: illumination spectrum. (b) Monochrome image of a black-on-white scattering resolution target.

Fig. 2.
Fig. 2.

Acquiring spectra using SDISI. A sample comprising a red star and a green circle is scanned across the spectrally dispersed illumination, resulting in the gradual capture of the reflection spectrum from each sample location. Star (circle) markers correspond to the reflection intensity from the red star (green circle).

Fig. 3.
Fig. 3.

(a) Averaged SDISI spectra (blue curves) of a color calibration target compared to spectra measured by a commercial spectrometer (red curves). (b) Corresponding colors of the SDISI curved spectra in (a) show lack of blue hues. (c) A color photograph of the target.

Fig. 4.
Fig. 4.

Selected reflectance spectra (blue curves) from SDISI of a volunteer’s finger. Spectrum of oxyhemoglobin (gray curve) is shown in the top right plot for reference.

Equations (1)

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SNRSDISIQerImaxshνtDt=QerImaxsThνD1N+M.

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