Abstract

We developed a reflection-mode optoacoustic mesoscopy system, based on raster-scanning of a custom designed spherically focused ultrasound detector, enabling seamless epi-illumination of the volume imaged. We study the performance of acoustic-resolution mesoscopy operating at an ultrawideband bandwidth of 20–180 MHz. i.e., a frequency band spreading over virtually an order of magnitude. Using tomographic reconstruction we showcase previously unreported, to our knowledge, axial resolutions of 4 μm and transverse resolutions of 18 μm reaching depths of up to 5 mm. We further investigate the frequency-dependence of features seen on the images to understand the implications of ultrawideband measurements. We show the overall imaging performance and the frequency ranges that contribute to observable resolution improvements from phantoms and animals.

© 2014 Optical Society of America

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    [CrossRef]
  9. S. L. Chen, Z. Xie, L. J. Guo, and X. Wang, Photoacoustics 1, 30 (2013).
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]

2014 (1)

2013 (6)

P. Keller, Science 340, 6137 (2013).
[CrossRef]

M. Omar, J. Gateau, and V. Ntziachristos, Opt. Lett. 38, 2472 (2013).
[CrossRef]

E. Strohm, E. Berndl, and M. Kolios, Photoacoustics 1, 49 (2013).

J. Yao and L. V. Wang, Laser Photon. Rev. 7, 758 (2013).

S. L. Chen, Z. Xie, L. J. Guo, and X. Wang, Photoacoustics 1, 30 (2013).

J. Gateau, A. Chekkoury, and V. Ntziachristos, J. Biomed. Opt. 18, 106005 (2013).
[CrossRef]

2012 (2)

Z. Deng, X. Yang, H. Gong, and Q. Luo, Opt. Express 20, 7555 (2012).
[CrossRef]

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

2011 (2)

2010 (1)

V. Ntziachristos, Nat. Methods 7, 603 (2010).
[CrossRef]

2008 (1)

2006 (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

1995 (1)

M. I. Khan and G. J. Diebold, Ultrasonics 33, 265 (1995).
[CrossRef]

Alex, A.

Beard, P.

Beaurepaire, E.

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

Berndl, E.

E. Strohm, E. Berndl, and M. Kolios, Photoacoustics 1, 49 (2013).

Chekkoury, A.

J. Gateau, A. Chekkoury, and V. Ntziachristos, J. Biomed. Opt. 18, 106005 (2013).
[CrossRef]

Chen, S. L.

S. L. Chen, Z. Xie, L. J. Guo, and X. Wang, Photoacoustics 1, 30 (2013).

Cox, B.

Dean-Ben, X. L.

X. L. Dean-Ben, D. Razansky, and V. Ntziachristos, Phys. Med. Biol. 56, 6129 (2011).
[CrossRef]

Débarre, D.

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

Deng, Z.

Diebold, G. J.

M. I. Khan and G. J. Diebold, Ultrasonics 33, 265 (1995).
[CrossRef]

Drexler, W.

Gateau, J.

M. Omar, J. Gateau, and V. Ntziachristos, Opt. Lett. 38, 2472 (2013).
[CrossRef]

J. Gateau, A. Chekkoury, and V. Ntziachristos, J. Biomed. Opt. 18, 106005 (2013).
[CrossRef]

Glittenberg, C.

Gong, H.

Guo, L. J.

S. L. Chen, Z. Xie, L. J. Guo, and X. Wang, Photoacoustics 1, 30 (2013).

Hofer, B.

Hu, S.

Keller, P.

P. Keller, Science 340, 6137 (2013).
[CrossRef]

Khan, M. I.

M. I. Khan and G. J. Diebold, Ultrasonics 33, 265 (1995).
[CrossRef]

Kolios, M.

E. Strohm, E. Berndl, and M. Kolios, Photoacoustics 1, 49 (2013).

Labroille, G.

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

Laufer, J.

Livet, J.

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

Loulier, K.

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

Luo, Q.

Mahou, P.

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

Maslov, K.

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Matho, K. S.

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

Morin, X.

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

Ntziachristos, V.

G. J. Tserevelakis, D. Soliman, M. Omar, and V. Ntziachristos, Opt. Lett. 39, 1819 (2014).
[CrossRef]

M. Omar, J. Gateau, and V. Ntziachristos, Opt. Lett. 38, 2472 (2013).
[CrossRef]

J. Gateau, A. Chekkoury, and V. Ntziachristos, J. Biomed. Opt. 18, 106005 (2013).
[CrossRef]

X. L. Dean-Ben, D. Razansky, and V. Ntziachristos, Phys. Med. Biol. 56, 6129 (2011).
[CrossRef]

V. Ntziachristos, Nat. Methods 7, 603 (2010).
[CrossRef]

Omar, M.

Pedley, B.

Povazay, B.

Razansky, D.

X. L. Dean-Ben, D. Razansky, and V. Ntziachristos, Phys. Med. Biol. 56, 6129 (2011).
[CrossRef]

Soliman, D.

Stoica, G.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Strohm, E.

E. Strohm, E. Berndl, and M. Kolios, Photoacoustics 1, 49 (2013).

Supatto, W.

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

Treeby, B.

Tserevelakis, G. J.

Wang, L. V.

J. Yao and L. V. Wang, Laser Photon. Rev. 7, 758 (2013).

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Wang, X.

S. L. Chen, Z. Xie, L. J. Guo, and X. Wang, Photoacoustics 1, 30 (2013).

Xie, Z.

S. L. Chen, Z. Xie, L. J. Guo, and X. Wang, Photoacoustics 1, 30 (2013).

Yang, X.

Yao, J.

J. Yao and L. V. Wang, Laser Photon. Rev. 7, 758 (2013).

Zhang, E. Z.

Zhang, H. F.

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Zimmerley, M.

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

Biomed. Opt. Express (1)

J. Biomed. Opt. (1)

J. Gateau, A. Chekkoury, and V. Ntziachristos, J. Biomed. Opt. 18, 106005 (2013).
[CrossRef]

Laser Photon. Rev. (1)

J. Yao and L. V. Wang, Laser Photon. Rev. 7, 758 (2013).

Nat. Biotechnol. (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Nat. Methods (2)

P. Mahou, M. Zimmerley, K. Loulier, K. S. Matho, G. Labroille, X. Morin, W. Supatto, J. Livet, D. Débarre, and E. Beaurepaire, Nat. Methods 9, 815 (2012).
[CrossRef]

V. Ntziachristos, Nat. Methods 7, 603 (2010).
[CrossRef]

Opt. Express (1)

Opt. Lett. (3)

Photoacoustics (2)

S. L. Chen, Z. Xie, L. J. Guo, and X. Wang, Photoacoustics 1, 30 (2013).

E. Strohm, E. Berndl, and M. Kolios, Photoacoustics 1, 49 (2013).

Phys. Med. Biol. (1)

X. L. Dean-Ben, D. Razansky, and V. Ntziachristos, Phys. Med. Biol. 56, 6129 (2011).
[CrossRef]

Science (1)

P. Keller, Science 340, 6137 (2013).
[CrossRef]

Ultrasonics (1)

M. I. Khan and G. J. Diebold, Ultrasonics 33, 265 (1995).
[CrossRef]

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Figures (4)

Fig. 1.
Fig. 1.

(a) Sketch of the previous R-SOM implementation, (b) design of the new detector, with a customized conically shaped lens to allow for light to be coupled to the sample at an angle of 45°. [In (a) the violet disk is a diffusor; in (b) the violet rods are arms of a fiber bundle.]

Fig. 2.
Fig. 2.

(a) MIP of 3 μm spheres at depths of 75–125 μm away from the focus, (b) horizontal (H), vertical (V), and axial (A) profiles through one of the microspheres in (a), (c) MIP of a 10 μm suture bent into a knot at a depth of 5 mm, (d) cross-section through the knot at different depths along the yellow line. Scale bar: (c) 1 mm.

Fig. 3.
Fig. 3.

(a) MIP of a zebrafish (ZF), (E) eye, (IH) intestines and heart, (M) melanocytes, (LL) lateral line, (b) zoom-in, showing the full bandwidth, (c) zoom-in on the low frequencies, 20–100 MHz, (d) zoom-in on the high frequencies, 100–180 MHz, (e) zoom-in on the overlayed image, red total image, and white high frequency image. Scale bars: (a) 500 μm, (b–e) 250 μm.

Fig. 4.
Fig. 4.

(a) MIP of a mouse ear, the reconstruction was done using the full frequency range 20–180 MHz, (b) MIP of the mouse ear in the frequency band 20–100 MHz, (c) MIP of the mouse ear in the frequency band: 100–180 MHz, (d) overlay of (c) in white and (a) in red to emphasize high frequencies. Scale bar: 500 μm.

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