Abstract

This Letter presents an enhanced temporal focusing-based multiphoton excitation (MPE) microscope in which the conventional diffraction grating is replaced by a digital micromirror device (DMD). Experimental results from imaging a thin fluorescence film show that the 4.0 μm axial resolution of the microscope is comparable with that of a setup incorporating a 600lines/mm grating; hence, the optical sectioning ability of the proposed setup is demonstrated. Similar to a grating, the DMD diffracts illuminating light frequencies for temporal focusing; additionally, it generates arbitrary patterns. Since the DMD is placed on the image–conjugate plane of the objective lens’ focal plane, the MPE pattern can be projected on the focal plane precisely.

© 2014 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. B. R. Masters and P. T. C. So, Handbook of Biomedical Nonlinear Optical Microscopy (Oxford, 2008).
  2. P. T. C. So, E. Y. S. Yew, and C. Rowlands, Biophys. J. 105, 2641 (2013).
    [CrossRef]
  3. D. Oron, E. Tal, and Y. Silberberg, Opt. Express 13, 1468 (2005).
    [CrossRef]
  4. G. Zhu, J. V. Howe, M. Durst, W. Zipfel, and C. Xu, Opt. Express 13, 2153 (2005).
    [CrossRef]
  5. A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, Proc. Natl. Acad. Sci. U. S. A. 105, 20221 (2008).
    [CrossRef]
  6. E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, Nat. Methods 7, 848 (2010).
    [CrossRef]
  7. D. Kim and P. T. C. So, Opt. Lett. 35, 1602 (2010).
    [CrossRef]
  8. O. D. Therrien, B. Aubé, S. Pagès, P. De Koninck, and D. Côté, Biomed. Opt. Express 2, 696 (2011).
    [CrossRef]
  9. L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, Opt. Express 20, 8939 (2012).
    [CrossRef]
  10. J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
    [CrossRef]
  11. H. Dana and S. Shoham, Opt. Lett. 37, 2913 (2012).
    [CrossRef]
  12. P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, Nat. Protoc. 7, 1410 (2012).
    [CrossRef]
  13. D. N. Vitek, D. E. Adams, A. Johnson, P. S. Tsai, S. Backus, C. G. Durfee, D. Kleinfeld, and J. A. Squier, Opt. Express 18, 18086 (2010).
    [CrossRef]
  14. Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-H. Lien, P. J. Campagnola, and S.-J. Chen, Opt. Express 20, 19030 (2012).
    [CrossRef]
  15. T. Kreis, P. Aswendt, and R. Höfling, Opt. Eng. 40, 926 (2001).
    [CrossRef]
  16. D. Dudley, W. Duncan, and J. Slaughter, Proc. SPIE 4985, 14 (2003).
    [CrossRef]
  17. P. J. Rodrigo, I. R. Perch-Nielsen, and J. Glückstad, Opt. Express 14, 5588 (2006).
    [CrossRef]
  18. Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, J. Biomed. Opt. 18, 075004 (2013).
    [CrossRef]
  19. E. Y. S. Yew, C. J. R. Sheppard, and P. T. C. So, Opt. Express 21, 12951 (2013).
    [CrossRef]
  20. D. Lim, K. K. Chu, and J. Mertz, Opt. Lett. 33, 1819 (2008).
    [CrossRef]
  21. M. G. L. Gustafsson, J. Microsc. 198, 82 (2000).
    [CrossRef]

2013 (3)

P. T. C. So, E. Y. S. Yew, and C. Rowlands, Biophys. J. 105, 2641 (2013).
[CrossRef]

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, J. Biomed. Opt. 18, 075004 (2013).
[CrossRef]

E. Y. S. Yew, C. J. R. Sheppard, and P. T. C. So, Opt. Express 21, 12951 (2013).
[CrossRef]

2012 (4)

2011 (2)

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
[CrossRef]

O. D. Therrien, B. Aubé, S. Pagès, P. De Koninck, and D. Côté, Biomed. Opt. Express 2, 696 (2011).
[CrossRef]

2010 (3)

2008 (2)

D. Lim, K. K. Chu, and J. Mertz, Opt. Lett. 33, 1819 (2008).
[CrossRef]

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, Proc. Natl. Acad. Sci. U. S. A. 105, 20221 (2008).
[CrossRef]

2006 (1)

2005 (2)

2003 (1)

D. Dudley, W. Duncan, and J. Slaughter, Proc. SPIE 4985, 14 (2003).
[CrossRef]

2001 (1)

T. Kreis, P. Aswendt, and R. Höfling, Opt. Eng. 40, 926 (2001).
[CrossRef]

2000 (1)

M. G. L. Gustafsson, J. Microsc. 198, 82 (2000).
[CrossRef]

Adams, D. E.

Anselmi, F.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, Nat. Methods 7, 848 (2010).
[CrossRef]

Aswendt, P.

T. Kreis, P. Aswendt, and R. Höfling, Opt. Eng. 40, 926 (2001).
[CrossRef]

Aubé, B.

Backus, S.

Bègue, A.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, Nat. Methods 7, 848 (2010).
[CrossRef]

Blake, G. A.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
[CrossRef]

Campagnola, P. J.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, J. Biomed. Opt. 18, 075004 (2013).
[CrossRef]

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-H. Lien, P. J. Campagnola, and S.-J. Chen, Opt. Express 20, 19030 (2012).
[CrossRef]

Chang, C.-Y.

Chang, N.-S.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, J. Biomed. Opt. 18, 075004 (2013).
[CrossRef]

L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, Opt. Express 20, 8939 (2012).
[CrossRef]

Chen, S.-J.

Chen, Y.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
[CrossRef]

Cheng, L.-C.

Cho, K.-C.

Chu, K. K.

Côté, D.

Dana, H.

De Koninck, P.

de Sars, V.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, Nat. Methods 7, 848 (2010).
[CrossRef]

Dong, C. Y.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, J. Biomed. Opt. 18, 075004 (2013).
[CrossRef]

L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, Opt. Express 20, 8939 (2012).
[CrossRef]

Dudley, D.

D. Dudley, W. Duncan, and J. Slaughter, Proc. SPIE 4985, 14 (2003).
[CrossRef]

Duncan, W.

D. Dudley, W. Duncan, and J. Slaughter, Proc. SPIE 4985, 14 (2003).
[CrossRef]

Durfee, C. G.

Durst, M.

Emiliani, V.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, Nat. Methods 7, 848 (2010).
[CrossRef]

Fajardo, O.

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, Nat. Protoc. 7, 1410 (2012).
[CrossRef]

Friedrich, R. W.

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, Nat. Protoc. 7, 1410 (2012).
[CrossRef]

Glückstad, J.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, Nat. Methods 7, 848 (2010).
[CrossRef]

P. J. Rodrigo, I. R. Perch-Nielsen, and J. Glückstad, Opt. Express 14, 5588 (2006).
[CrossRef]

Guo, C.-L.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
[CrossRef]

Gustafsson, M. G. L.

M. G. L. Gustafsson, J. Microsc. 198, 82 (2000).
[CrossRef]

Höfling, R.

T. Kreis, P. Aswendt, and R. Höfling, Opt. Eng. 40, 926 (2001).
[CrossRef]

Holland, D. B.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
[CrossRef]

Howe, J. V.

Isacoff, E. Y.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, Nat. Methods 7, 848 (2010).
[CrossRef]

Johnson, A.

Kim, D.

Kleinfeld, D.

Kreis, T.

T. Kreis, P. Aswendt, and R. Höfling, Opt. Eng. 40, 926 (2001).
[CrossRef]

Kuo, C.-H.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
[CrossRef]

Li, Y.-C.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, J. Biomed. Opt. 18, 075004 (2013).
[CrossRef]

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-H. Lien, P. J. Campagnola, and S.-J. Chen, Opt. Express 20, 19030 (2012).
[CrossRef]

Lien, C.-H.

Lim, D.

Lin, C.-Y.

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, J. Biomed. Opt. 18, 075004 (2013).
[CrossRef]

L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, Opt. Express 20, 8939 (2012).
[CrossRef]

Masters, B. R.

B. R. Masters and P. T. C. So, Handbook of Biomedical Nonlinear Optical Microscopy (Oxford, 2008).

Mertz, J.

Oron, D.

Ouyang, M.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
[CrossRef]

Pagès, S.

Papagiakoumou, E.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, Nat. Methods 7, 848 (2010).
[CrossRef]

Perch-Nielsen, I. R.

Rodrigo, P. J.

Rowlands, C.

P. T. C. So, E. Y. S. Yew, and C. Rowlands, Biophys. J. 105, 2641 (2013).
[CrossRef]

Shank, C. V.

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, Proc. Natl. Acad. Sci. U. S. A. 105, 20221 (2008).
[CrossRef]

Sheppard, C. J. R.

Shoham, S.

Shroff, H.

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, Proc. Natl. Acad. Sci. U. S. A. 105, 20221 (2008).
[CrossRef]

Shum, J.

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, Nat. Protoc. 7, 1410 (2012).
[CrossRef]

Silberberg, Y.

Slaughter, J.

D. Dudley, W. Duncan, and J. Slaughter, Proc. SPIE 4985, 14 (2003).
[CrossRef]

So, P. T. C.

P. T. C. So, E. Y. S. Yew, and C. Rowlands, Biophys. J. 105, 2641 (2013).
[CrossRef]

E. Y. S. Yew, C. J. R. Sheppard, and P. T. C. So, Opt. Express 21, 12951 (2013).
[CrossRef]

D. Kim and P. T. C. So, Opt. Lett. 35, 1602 (2010).
[CrossRef]

B. R. Masters and P. T. C. So, Handbook of Biomedical Nonlinear Optical Microscopy (Oxford, 2008).

Squier, J. A.

Tal, E.

Tang, J.

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, Proc. Natl. Acad. Sci. U. S. A. 105, 20221 (2008).
[CrossRef]

Therrien, O. D.

Tsai, P. S.

Vaziri, A.

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, Proc. Natl. Acad. Sci. U. S. A. 105, 20221 (2008).
[CrossRef]

Vitek, D. N.

Xu, C.

Yen, W.-C.

Yew, E. Y. S.

E. Y. S. Yew, C. J. R. Sheppard, and P. T. C. So, Opt. Express 21, 12951 (2013).
[CrossRef]

P. T. C. So, E. Y. S. Yew, and C. Rowlands, Biophys. J. 105, 2641 (2013).
[CrossRef]

Yu, J.-Y.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
[CrossRef]

Zadoyan, R.

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
[CrossRef]

Zhang Schärer, Y.-P.

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, Nat. Protoc. 7, 1410 (2012).
[CrossRef]

Zhu, G.

Zhu, P.

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, Nat. Protoc. 7, 1410 (2012).
[CrossRef]

Zipfel, W.

Biomed. Opt. Express (1)

Biophys. J. (1)

P. T. C. So, E. Y. S. Yew, and C. Rowlands, Biophys. J. 105, 2641 (2013).
[CrossRef]

J. Biomed. Opt. (2)

J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011).
[CrossRef]

Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, J. Biomed. Opt. 18, 075004 (2013).
[CrossRef]

J. Microsc. (1)

M. G. L. Gustafsson, J. Microsc. 198, 82 (2000).
[CrossRef]

Nat. Methods (1)

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, Nat. Methods 7, 848 (2010).
[CrossRef]

Nat. Protoc. (1)

P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, Nat. Protoc. 7, 1410 (2012).
[CrossRef]

Opt. Eng. (1)

T. Kreis, P. Aswendt, and R. Höfling, Opt. Eng. 40, 926 (2001).
[CrossRef]

Opt. Express (7)

Opt. Lett. (3)

Proc. Natl. Acad. Sci. U. S. A. (1)

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, Proc. Natl. Acad. Sci. U. S. A. 105, 20221 (2008).
[CrossRef]

Proc. SPIE (1)

D. Dudley, W. Duncan, and J. Slaughter, Proc. SPIE 4985, 14 (2003).
[CrossRef]

Other (1)

B. R. Masters and P. T. C. So, Handbook of Biomedical Nonlinear Optical Microscopy (Oxford, 2008).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (3)

Fig. 1.
Fig. 1.

Schematic of the temporal focusing-based MPE microscope using a DMD. The red dashed lines represent the mutual image–conjugate planes between the DMD surface and the objective lens’ focal plane.

Fig. 2.
Fig. 2.

(a) Axial MPE fluorescence intensity profiles of the thin film using a 600 lines / mm blazed grating and the DMD, separately. The axial confinement of the system with the DMD is 4.0 μm while that of the system with the grating is 3.0 μm. (b) Diffraction efficiency of different polarization light on the DMD and the blazed grating.

Fig. 3.
Fig. 3.

(a) Axial MPE fluorescence intensity profiles of the thin film with a uniform pattern, an alternating horizontal periodic pattern, and a standard USAF target, separately. The axial resolutions for the different patterns are all equal to around 4.0 μm. The MPE fluorescence images when the PMMA thin film is placed on the focal plane with (b) the alternating horizontal periodic pattern, (c) the USAF target, and (d) a plane 5 μm from focal plane to the USAF target.

Equations (1)

Equations on this page are rendered with MathJax. Learn more.

m λ = a ( sin θ i + sin θ m ) ,

Metrics