Abstract

With respect to experimental condition, we have investigated the point spread function of a high numerical aperture objective lens, taking into account the absorption effect of the studied material. By using a material possessing an ultralow one-photon absorption (LOPA) coefficient at the excitation wavelength, the light beam can penetrate deeply inside the material and be tightly focused into a subwavelength spot, almost the same as in the absence of material. Combining tight focusing and ultralow absorption conditions, we show that LOPA-based microscopy is thus capable of three-dimensional imaging and fabrication with long penetration depth up to 300 μm. As compared to the commonly used two-photon absorption microscope, the LOPA method allows simplification of the experimental setup and also minimization of the photodamaging or bleaching effect of materials.

© 2013 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Submicrometer 3D structures fabrication enabled by one-photon absorption direct laser writing

Mai Trang Do, Thi Thanh Ngan Nguyen, Qinggele Li, Henri Benisty, Isabelle Ledoux-Rak, and Ngoc Diep Lai
Opt. Express 21(18) 20964-20973 (2013)

Reduced deep-tissue image degradation in three-dimensional multiphoton microscopy with concentric two-color two-photon fluorescence excitation

Chen Wang, Lingling Qiao, Zhengle Mao, Ya Cheng, and Zhizhan Xu
J. Opt. Soc. Am. B 25(6) 976-982 (2008)

Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics

Brian R. Patton, Daniel Burke, David Owald, Travis J. Gould, Joerg Bewersdorf, and Martin J. Booth
Opt. Express 24(8) 8862-8876 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (3)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription