Abstract

This study was designed to test the feasibility of en face mapping of the transient intrinsic optical signal (IOS) response at photoreceptor outer segments and to assess the effect of spatial resolution on functional IOS imaging of retinal photoreceptors. A line-scan optical coherence tomography (LS-OCT) was constructed to achieve depth-resolved functional IOS imaging of living frog eyecups. Rapid en face OCT revealed transient IOS almost immediately (<3ms) after the onset of visible light flashes at photoreceptor outer segments. Quantitative analysis indicated that the IOS kinetics may reflect dynamics of G-protein binding and releasing in early phases of visual transduction, and high resolution is essential to differentiate positive and negative IOS changes in adjacent locations.

© 2013 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Functional optical coherence tomography reveals transient phototropic change of photoreceptor outer segments

Benquan Wang, Qiuxiang Zhang, Rongwen Lu, Yanan Zhi, and Xincheng Yao
Opt. Lett. 39(24) 6923-6926 (2014)

Stimulus-evoked outer segment changes occur before the hyperpolarization of retinal photoreceptors

Yiming Lu, Benquan Wang, David R. Pepperberg, and Xincheng Yao
Biomed. Opt. Express 8(1) 38-47 (2017)

Adaptive optics parallel spectral domain optical coherence tomography for imaging the living retina

Yan Zhang, Jungtae Rha, Ravi S. Jonnal, and Donald T. Miller
Opt. Express 13(12) 4792-4811 (2005)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription