Abstract

We developed a dual-wavelength laser speckle imaging system using a single industrial-grade color CCD camera with Bayer filters to simultaneously image changes in blood flow, blood volume, and oxygenation. One frame of a color image recorded with dual-wavelength laser illumination provides not only the intensity fluctuation of the speckle pattern, but also the dual-wavelength optical reflectance signal. The method was validated using a tissue phantom and cuff ischemia experiments in the human arm. This system achieves complete time synchronization, unlike conventional time-sharing systems. Compared with a multicamera system, it also avoids the problem of image registration and can be less expensive.

© 2013 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. A. K. Dunn, A. Devor, H. Bolay, M. L. Andermann, M. A. Moskowitz, A. M. Dale, and D. A. Boas, Opt. Lett. 28, 28 (2003).
    [CrossRef]
  2. Z. Luo, Z. Yuan, Y. Pan, and C. Du, Opt. Lett. 34, 1480 (2009).
    [CrossRef]
  3. J. Qin, L. Shi, S. Dziennis, R. Reif, and R. K. Wang, Opt. Lett. 37, 4005 (2012).
    [CrossRef]
  4. O. Yang and B. Choi, Opt. Lett. 37, 3957 (2012).
    [CrossRef]
  5. J. D. Briers and S. Webster, J. Biomed. Opt. 1, 174 (1996).
    [CrossRef]
  6. D. A. Boas and A. K. Dunn, J. Biomed. Opt. 15, 011109 (2010).
    [CrossRef]
  7. R. Bandyopadhyay, A. S. Gittings, S. S. Suh, P. K. Dixon, and D. J. Durian, Rev. Sci. Instrum. 76, 093110 (2005).
    [CrossRef]
  8. M. Jones, J. Berwick, D. Johnston, and J. Mayhew, NeuroImage 13, 1002 (2001).
    [CrossRef]
  9. M. Kohl, U. Lindauer, G. Royl, M. Kuhl, L. Gold, A. Villringer, and U. Dirnagl, Phys. Med. Biol. 45, 3749 (2000).
    [CrossRef]
  10. D. A. Boas, “Diffuse photon probes of structural and dynamical properties of turbid media: theory and biomedical applications,” Ph.D. dissertation (University of Pennsylvania, 1996).
  11. S. J. Kirkpatrick, D. D. Duncan, and E. M. Wells-Gray, Opt. Lett. 33, 2886 (2008).
    [CrossRef]

2012 (2)

2010 (1)

D. A. Boas and A. K. Dunn, J. Biomed. Opt. 15, 011109 (2010).
[CrossRef]

2009 (1)

2008 (1)

2005 (1)

R. Bandyopadhyay, A. S. Gittings, S. S. Suh, P. K. Dixon, and D. J. Durian, Rev. Sci. Instrum. 76, 093110 (2005).
[CrossRef]

2003 (1)

2001 (1)

M. Jones, J. Berwick, D. Johnston, and J. Mayhew, NeuroImage 13, 1002 (2001).
[CrossRef]

2000 (1)

M. Kohl, U. Lindauer, G. Royl, M. Kuhl, L. Gold, A. Villringer, and U. Dirnagl, Phys. Med. Biol. 45, 3749 (2000).
[CrossRef]

1996 (1)

J. D. Briers and S. Webster, J. Biomed. Opt. 1, 174 (1996).
[CrossRef]

Andermann, M. L.

Bandyopadhyay, R.

R. Bandyopadhyay, A. S. Gittings, S. S. Suh, P. K. Dixon, and D. J. Durian, Rev. Sci. Instrum. 76, 093110 (2005).
[CrossRef]

Berwick, J.

M. Jones, J. Berwick, D. Johnston, and J. Mayhew, NeuroImage 13, 1002 (2001).
[CrossRef]

Boas, D. A.

D. A. Boas and A. K. Dunn, J. Biomed. Opt. 15, 011109 (2010).
[CrossRef]

A. K. Dunn, A. Devor, H. Bolay, M. L. Andermann, M. A. Moskowitz, A. M. Dale, and D. A. Boas, Opt. Lett. 28, 28 (2003).
[CrossRef]

D. A. Boas, “Diffuse photon probes of structural and dynamical properties of turbid media: theory and biomedical applications,” Ph.D. dissertation (University of Pennsylvania, 1996).

Bolay, H.

Briers, J. D.

J. D. Briers and S. Webster, J. Biomed. Opt. 1, 174 (1996).
[CrossRef]

Choi, B.

Dale, A. M.

Devor, A.

Dirnagl, U.

M. Kohl, U. Lindauer, G. Royl, M. Kuhl, L. Gold, A. Villringer, and U. Dirnagl, Phys. Med. Biol. 45, 3749 (2000).
[CrossRef]

Dixon, P. K.

R. Bandyopadhyay, A. S. Gittings, S. S. Suh, P. K. Dixon, and D. J. Durian, Rev. Sci. Instrum. 76, 093110 (2005).
[CrossRef]

Du, C.

Duncan, D. D.

Dunn, A. K.

Durian, D. J.

R. Bandyopadhyay, A. S. Gittings, S. S. Suh, P. K. Dixon, and D. J. Durian, Rev. Sci. Instrum. 76, 093110 (2005).
[CrossRef]

Dziennis, S.

Gittings, A. S.

R. Bandyopadhyay, A. S. Gittings, S. S. Suh, P. K. Dixon, and D. J. Durian, Rev. Sci. Instrum. 76, 093110 (2005).
[CrossRef]

Gold, L.

M. Kohl, U. Lindauer, G. Royl, M. Kuhl, L. Gold, A. Villringer, and U. Dirnagl, Phys. Med. Biol. 45, 3749 (2000).
[CrossRef]

Johnston, D.

M. Jones, J. Berwick, D. Johnston, and J. Mayhew, NeuroImage 13, 1002 (2001).
[CrossRef]

Jones, M.

M. Jones, J. Berwick, D. Johnston, and J. Mayhew, NeuroImage 13, 1002 (2001).
[CrossRef]

Kirkpatrick, S. J.

Kohl, M.

M. Kohl, U. Lindauer, G. Royl, M. Kuhl, L. Gold, A. Villringer, and U. Dirnagl, Phys. Med. Biol. 45, 3749 (2000).
[CrossRef]

Kuhl, M.

M. Kohl, U. Lindauer, G. Royl, M. Kuhl, L. Gold, A. Villringer, and U. Dirnagl, Phys. Med. Biol. 45, 3749 (2000).
[CrossRef]

Lindauer, U.

M. Kohl, U. Lindauer, G. Royl, M. Kuhl, L. Gold, A. Villringer, and U. Dirnagl, Phys. Med. Biol. 45, 3749 (2000).
[CrossRef]

Luo, Z.

Mayhew, J.

M. Jones, J. Berwick, D. Johnston, and J. Mayhew, NeuroImage 13, 1002 (2001).
[CrossRef]

Moskowitz, M. A.

Pan, Y.

Qin, J.

Reif, R.

Royl, G.

M. Kohl, U. Lindauer, G. Royl, M. Kuhl, L. Gold, A. Villringer, and U. Dirnagl, Phys. Med. Biol. 45, 3749 (2000).
[CrossRef]

Shi, L.

Suh, S. S.

R. Bandyopadhyay, A. S. Gittings, S. S. Suh, P. K. Dixon, and D. J. Durian, Rev. Sci. Instrum. 76, 093110 (2005).
[CrossRef]

Villringer, A.

M. Kohl, U. Lindauer, G. Royl, M. Kuhl, L. Gold, A. Villringer, and U. Dirnagl, Phys. Med. Biol. 45, 3749 (2000).
[CrossRef]

Wang, R. K.

Webster, S.

J. D. Briers and S. Webster, J. Biomed. Opt. 1, 174 (1996).
[CrossRef]

Wells-Gray, E. M.

Yang, O.

Yuan, Z.

J. Biomed. Opt. (2)

J. D. Briers and S. Webster, J. Biomed. Opt. 1, 174 (1996).
[CrossRef]

D. A. Boas and A. K. Dunn, J. Biomed. Opt. 15, 011109 (2010).
[CrossRef]

NeuroImage (1)

M. Jones, J. Berwick, D. Johnston, and J. Mayhew, NeuroImage 13, 1002 (2001).
[CrossRef]

Opt. Lett. (5)

Phys. Med. Biol. (1)

M. Kohl, U. Lindauer, G. Royl, M. Kuhl, L. Gold, A. Villringer, and U. Dirnagl, Phys. Med. Biol. 45, 3749 (2000).
[CrossRef]

Rev. Sci. Instrum. (1)

R. Bandyopadhyay, A. S. Gittings, S. S. Suh, P. K. Dixon, and D. J. Durian, Rev. Sci. Instrum. 76, 093110 (2005).
[CrossRef]

Other (1)

D. A. Boas, “Diffuse photon probes of structural and dynamical properties of turbid media: theory and biomedical applications,” Ph.D. dissertation (University of Pennsylvania, 1996).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (3)

Fig. 1.
Fig. 1.

(a) Schematic of the experimental setup. (b) Process of separating data from the raw image. (c) Relative response of Bayer filters in the visible band. (d) Molar extinction coefficients of oxy-hemoglobin and deoxy-hemoglobin.

Fig. 2.
Fig. 2.

(a) Changes in concentrations of total hemoglobin obtained from the experiments adding blood into phantom solution with two-wavelength DRS using monochrome camera and dual-wavelength LSCI using color camera, respectively. (b) The results obtained from the experiments feeding oxygen into phantom solution with two-wavelength DRS using monochrome camera and dual-wavelength LSCI using color camera, respectively.

Fig. 3.
Fig. 3.

Spatiotemporal changes in [HbO], [HbR], [HbT], and blood flow obtained by dual-wavelength LSCI in a cuff experiment.

Equations (2)

Equations on this page are rendered with MathJax. Learn more.

K2=τcT+τc22T2[exp(2T/τc)1)].
[Δ[HbO2(t)]Δ[HbR(t)]]=[εHbO2532εHbR532εHbO2633εHbR633]1[ln(R532(0)/R532(t))DP532ln(R633(0)/R633(t))DP633],

Metrics