Abstract

The use of femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples is presented. A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.

© 2013 Optical Society of America

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References

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    [CrossRef]

2013 (2)

2012 (4)

J. Garcia-Sucerquia, Opt. Lett. 37, 1724 (2012).
[CrossRef]

S. Witte, A. Plauska, M. C. Ridder, L. van Berge, H. D. Mansvelder, and M. L. Groot, Biomed. Opt. Express 3, 2184 (2012).
[CrossRef]

P. Petruck, R. Riesenberg, and R. Kowarschik, Appl. Phys. B 106, 339 (2012).
[CrossRef]

M. Brunel, H. Shen, S. Coetmellec, D. Lebrun, and K. Ait Ameur, Appl. Phys. B 106, 583 (2012).
[CrossRef]

2011 (1)

2009 (1)

2006 (1)

Ait Ameur, K.

M. Brunel, H. Shen, S. Coetmellec, D. Lebrun, and K. Ait Ameur, Appl. Phys. B 106, 583 (2012).
[CrossRef]

Andrés, P.

Bishara, W.

Brunel, M.

M. Brunel, H. Shen, S. Coetmellec, D. Lebrun, and K. Ait Ameur, Appl. Phys. B 106, 583 (2012).
[CrossRef]

Clemente, P.

Coetmellec, S.

M. Brunel, H. Shen, S. Coetmellec, D. Lebrun, and K. Ait Ameur, Appl. Phys. B 106, 583 (2012).
[CrossRef]

Garcia-Sucerquia, J.

Groot, M. L.

Gu, M.

M. Gu, Advanced Optical Imaging Theory, Vol. 75 of Springer Series in Optical Sciences (Springer, 2000).

Isikman, S. O.

Jericho, M. H.

J. Garcia-Sucerquia, W. Xu, S. K. Jericho, P. Klages, M. H. Jericho, and H. J. Kreuzer, Appl. Opt. 45, 836 (2006).
[CrossRef]

M. H. Jericho and H. J. Kreuzer, in Coherent Light Microscopy, P. Ferraro, A. Wax, and Z. Zalevvsky, eds., Springer Series in Surface Sciences (Springer, 2011), pp. 3–30.

Jericho, S. K.

Klages, P.

Kowarschik, R.

P. Petruck, R. Riesenberg, and R. Kowarschik, Appl. Phys. B 106, 339 (2012).
[CrossRef]

Kreuzer, H. J.

J. Garcia-Sucerquia, W. Xu, S. K. Jericho, P. Klages, M. H. Jericho, and H. J. Kreuzer, Appl. Opt. 45, 836 (2006).
[CrossRef]

M. H. Jericho and H. J. Kreuzer, in Coherent Light Microscopy, P. Ferraro, A. Wax, and Z. Zalevvsky, eds., Springer Series in Surface Sciences (Springer, 2011), pp. 3–30.

Lancis, J.

Lebrun, D.

M. Brunel, H. Shen, S. Coetmellec, D. Lebrun, and K. Ait Ameur, Appl. Phys. B 106, 583 (2012).
[CrossRef]

Mansvelder, H. D.

Martínez-Cuenca, R.

Martínez-León, L.

Mendoza-Yero, O.

Mínguez-Vega, G.

Ozcan, A.

Petruck, P.

P. Petruck, R. Riesenberg, and R. Kowarschik, Appl. Phys. B 106, 339 (2012).
[CrossRef]

Plauska, A.

Ridder, M. C.

Riesenberg, R.

P. Petruck, R. Riesenberg, and R. Kowarschik, Appl. Phys. B 106, 339 (2012).
[CrossRef]

Shen, H.

M. Brunel, H. Shen, S. Coetmellec, D. Lebrun, and K. Ait Ameur, Appl. Phys. B 106, 583 (2012).
[CrossRef]

Tajahuerce, E.

van Berge, L.

Witte, S.

Xu, W.

Appl. Opt. (3)

Appl. Phys. B (2)

P. Petruck, R. Riesenberg, and R. Kowarschik, Appl. Phys. B 106, 339 (2012).
[CrossRef]

M. Brunel, H. Shen, S. Coetmellec, D. Lebrun, and K. Ait Ameur, Appl. Phys. B 106, 583 (2012).
[CrossRef]

Biomed. Opt. Express (1)

Opt. Express (1)

Opt. Lett. (2)

Other (2)

M. Gu, Advanced Optical Imaging Theory, Vol. 75 of Springer Series in Optical Sciences (Springer, 2000).

M. H. Jericho and H. J. Kreuzer, in Coherent Light Microscopy, P. Ferraro, A. Wax, and Z. Zalevvsky, eds., Springer Series in Surface Sciences (Springer, 2011), pp. 3–30.

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Figures (3)

Fig. 1.
Fig. 1.

Schematic setup for DLHM using both Ti:Sa laser and LED illumination.

Fig. 2.
Fig. 2.

Hologram reconstructions for the fs-DLHM and LED-DLHM. Panels (a) and (b) show the reconstructions for the fs-DLHM operating with pinholes of 5 and 1 μm in diameter, respectively. Panels (c) and (d) are the reconstructed holograms for the LED-DLHM using pinholes with diameters of 5 and 1 μm, in that order.

Fig. 3.
Fig. 3.

Spectra of the illuminations utilized in the fs-DLHM. The solid line corresponds to illumination with no pinhole. The dotted line and the dashed line illustrate the spectrum for the 5 and 1 μm pinholes, respectively.

Equations (3)

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I(r,λ)=|Uscat(r,λ)|2+|Uref(r,λ)|2+[Uscat(r,λ)Uref*(r,λ)+Uscat*(r,λ)Uref(r,λ)].
I(r)=λ0Δλ/2λ0+Δλ/2ρ(λ)I(r,λ)dλ,
I˜(r)=λ0Δλ/2λ0+Δλ/2ρ(λ)[I(r,λ)|Uref(r,λ)|2]dλ.

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