Abstract

We developed random-access optical-resolution photoacoustic microscopy using a digital micromirror device. This system can rapidly scan arbitrarily shaped regions of interest within a 40μm×40μm imaging area with a lateral resolution of 3.6 μm. To identify a region of interest, a global structural image is first acquired, then the selected region is scanned. The random-access ability was demonstrated by imaging two static samples, a carbon fiber cross and a monolayer of red blood cells, with an acquisition rate up to 4 kHz. The system was then used to monitor blood flow in vivo in real time within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 9.2 times.

© 2013 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Fast voice-coil scanning optical-resolution photoacoustic microscopy

Lidai Wang, Konstantin Maslov, Junjie Yao, Bin Rao, and Lihong V. Wang
Opt. Lett. 36(2) 139-141 (2011)

Reflection-mode optical-resolution photoacoustic microscopy based on a reflective objective

Hui Wang, Xiaoquan Yang, Yanyan Liu, Bowen Jiang, and Qingming Luo
Opt. Express 21(20) 24210-24218 (2013)

Fully motorized optical-resolution photoacoustic microscopy

Lei Li, Chenghung Yeh, Song Hu, Lidai Wang, Brian T. Soetikno, Ruimin Chen, Qifa Zhou, K. Kirk Shung, Konstantin I. Maslov, and Lihong V. Wang
Opt. Lett. 39(7) 2117-2120 (2014)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (8)

» Media 1: AVI (3131 KB)     
» Media 2: AVI (14973 KB)     
» Media 3: AVI (14745 KB)     
» Media 4: AVI (13041 KB)     
» Media 5: AVI (3926 KB)     
» Media 6: AVI (4095 KB)     
» Media 7: AVI (3297 KB)     
» Media 8: AVI (3822 KB)     

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription