Abstract

Due to strong light scattering in tissue, both the spatial resolution and maximum penetration depth of optical-resolution photoacoustic microscopy (OR-PAM) deteriorate sharply with depth. To reduce tissue scattering, we propose to use glycerol as an optical clearing agent in OR-PAM. Our results show that the imaging performance of OR-PAM can be greatly enhanced by optical clearing both in vitro and in vivo.

© 2013 Optical Society of America

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  2. Y. Zhou, C. Zhang, D.-K. Yao, and L. V. Wang, J. Biomed. Opt. 17, 126019 (2012).
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
  7. L. V. Wang and H. Wu, Biomedical Optics: Principles and Imaging (Wiley, 2007).
  8. D. Zhu, K. V. Larin, Q. Luo, and V. V. Tuchin, Laser & Photon. Rev. (2013) .
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    [CrossRef]
  10. R. Myllylä, Z. Zhao, and M. Kinnunen, in Handbook of Optical Sensing of Glucose in Biological Fluids and TissuesV. V. Tuchin, ed. (Taylor & Francis, 2008).
  11. V. V. Tuchin, J. Phys. D 38, 2497 (2005).
    [CrossRef]
  12. X. Wen, Z. Mao, Z. Han, V. V. Tuchin, and D. Zhu, J. Biophoton. 3, 44 (2010).
    [CrossRef]
  13. G. Vargas, A. Readinger, S. S. Dozier, and A. J. Welch, Photochem. Photobiol. 77541 (2003).
    [CrossRef]
  14. D. Zhu, J. Zhang, H. Cui, Z. Mao, P. Li, and Q. Luo, J. Biomed. Opt. 13, 021106 (2008).
    [CrossRef]
  15. D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, J. Biomed. Opt. 15, 026008 (2010).
    [CrossRef]
  16. J. Wang, R. Shi, and D. Zhu, J. Biomed. Opt. 18, 061209 (2013).
    [CrossRef]

2013 (1)

J. Wang, R. Shi, and D. Zhu, J. Biomed. Opt. 18, 061209 (2013).
[CrossRef]

2012 (2)

L. V. Wang and S. Hu, Science 335, 1458 (2012).
[CrossRef]

Y. Zhou, C. Zhang, D.-K. Yao, and L. V. Wang, J. Biomed. Opt. 17, 126019 (2012).
[CrossRef]

2011 (2)

J. Yao and L. V. Wang, Contrast Media Mol. Imag. 6, 332 (2011).
[CrossRef]

J. Yao, K. I. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, J. Biomed. Opt. 16, 076003 (2011).
[CrossRef]

2010 (4)

X. Wen, Z. Mao, Z. Han, V. V. Tuchin, and D. Zhu, J. Biophoton. 3, 44 (2010).
[CrossRef]

S. L. Chen, T. Ling, S. W. Huang, H. Won Baac, and L. J. Guo, Opt. Lett. 35, 1200 (2010).
[CrossRef]

J. Yao, K. I. Maslov, Y. Shi, L. A. Taber, and L. V. Wang, Opt. Lett. 35, 1419 (2010).
[CrossRef]

D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, J. Biomed. Opt. 15, 026008 (2010).
[CrossRef]

2008 (1)

D. Zhu, J. Zhang, H. Cui, Z. Mao, P. Li, and Q. Luo, J. Biomed. Opt. 13, 021106 (2008).
[CrossRef]

2005 (2)

M. Kinnunen and R. Myllylä, J. Phys. D 382654 (2005).
[CrossRef]

V. V. Tuchin, J. Phys. D 38, 2497 (2005).
[CrossRef]

2003 (1)

G. Vargas, A. Readinger, S. S. Dozier, and A. J. Welch, Photochem. Photobiol. 77541 (2003).
[CrossRef]

Chen, S. L.

Cui, H.

D. Zhu, J. Zhang, H. Cui, Z. Mao, P. Li, and Q. Luo, J. Biomed. Opt. 13, 021106 (2008).
[CrossRef]

Dozier, S. S.

G. Vargas, A. Readinger, S. S. Dozier, and A. J. Welch, Photochem. Photobiol. 77541 (2003).
[CrossRef]

Guo, L. J.

Han, Z.

X. Wen, Z. Mao, Z. Han, V. V. Tuchin, and D. Zhu, J. Biophoton. 3, 44 (2010).
[CrossRef]

Hu, S.

L. V. Wang and S. Hu, Science 335, 1458 (2012).
[CrossRef]

Huang, S. W.

Kinnunen, M.

M. Kinnunen and R. Myllylä, J. Phys. D 382654 (2005).
[CrossRef]

R. Myllylä, Z. Zhao, and M. Kinnunen, in Handbook of Optical Sensing of Glucose in Biological Fluids and TissuesV. V. Tuchin, ed. (Taylor & Francis, 2008).

Larin, K. V.

D. Zhu, K. V. Larin, Q. Luo, and V. V. Tuchin, Laser & Photon. Rev. (2013) .

Li, P.

D. Zhu, J. Zhang, H. Cui, Z. Mao, P. Li, and Q. Luo, J. Biomed. Opt. 13, 021106 (2008).
[CrossRef]

Ling, T.

Luo, Q.

D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, J. Biomed. Opt. 15, 026008 (2010).
[CrossRef]

D. Zhu, J. Zhang, H. Cui, Z. Mao, P. Li, and Q. Luo, J. Biomed. Opt. 13, 021106 (2008).
[CrossRef]

D. Zhu, K. V. Larin, Q. Luo, and V. V. Tuchin, Laser & Photon. Rev. (2013) .

Mao, Z.

X. Wen, Z. Mao, Z. Han, V. V. Tuchin, and D. Zhu, J. Biophoton. 3, 44 (2010).
[CrossRef]

D. Zhu, J. Zhang, H. Cui, Z. Mao, P. Li, and Q. Luo, J. Biomed. Opt. 13, 021106 (2008).
[CrossRef]

Maslov, K. I.

J. Yao, K. I. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, J. Biomed. Opt. 16, 076003 (2011).
[CrossRef]

J. Yao, K. I. Maslov, Y. Shi, L. A. Taber, and L. V. Wang, Opt. Lett. 35, 1419 (2010).
[CrossRef]

Myllylä, R.

M. Kinnunen and R. Myllylä, J. Phys. D 382654 (2005).
[CrossRef]

R. Myllylä, Z. Zhao, and M. Kinnunen, in Handbook of Optical Sensing of Glucose in Biological Fluids and TissuesV. V. Tuchin, ed. (Taylor & Francis, 2008).

Readinger, A.

G. Vargas, A. Readinger, S. S. Dozier, and A. J. Welch, Photochem. Photobiol. 77541 (2003).
[CrossRef]

Shi, R.

J. Wang, R. Shi, and D. Zhu, J. Biomed. Opt. 18, 061209 (2013).
[CrossRef]

Shi, Y.

Taber, L. A.

Tuchin, V. V.

X. Wen, Z. Mao, Z. Han, V. V. Tuchin, and D. Zhu, J. Biophoton. 3, 44 (2010).
[CrossRef]

V. V. Tuchin, J. Phys. D 38, 2497 (2005).
[CrossRef]

D. Zhu, K. V. Larin, Q. Luo, and V. V. Tuchin, Laser & Photon. Rev. (2013) .

Vargas, G.

G. Vargas, A. Readinger, S. S. Dozier, and A. J. Welch, Photochem. Photobiol. 77541 (2003).
[CrossRef]

Wang, J.

J. Wang, R. Shi, and D. Zhu, J. Biomed. Opt. 18, 061209 (2013).
[CrossRef]

D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, J. Biomed. Opt. 15, 026008 (2010).
[CrossRef]

Wang, L. V.

Y. Zhou, C. Zhang, D.-K. Yao, and L. V. Wang, J. Biomed. Opt. 17, 126019 (2012).
[CrossRef]

L. V. Wang and S. Hu, Science 335, 1458 (2012).
[CrossRef]

J. Yao and L. V. Wang, Contrast Media Mol. Imag. 6, 332 (2011).
[CrossRef]

J. Yao, K. I. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, J. Biomed. Opt. 16, 076003 (2011).
[CrossRef]

J. Yao, K. I. Maslov, Y. Shi, L. A. Taber, and L. V. Wang, Opt. Lett. 35, 1419 (2010).
[CrossRef]

L. V. Wang and H. Wu, Biomedical Optics: Principles and Imaging (Wiley, 2007).

Welch, A. J.

G. Vargas, A. Readinger, S. S. Dozier, and A. J. Welch, Photochem. Photobiol. 77541 (2003).
[CrossRef]

Wen, X.

D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, J. Biomed. Opt. 15, 026008 (2010).
[CrossRef]

X. Wen, Z. Mao, Z. Han, V. V. Tuchin, and D. Zhu, J. Biophoton. 3, 44 (2010).
[CrossRef]

Won Baac, H.

Wu, H.

L. V. Wang and H. Wu, Biomedical Optics: Principles and Imaging (Wiley, 2007).

Xia, Y.

J. Yao, K. I. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, J. Biomed. Opt. 16, 076003 (2011).
[CrossRef]

Yao, D.-K.

Y. Zhou, C. Zhang, D.-K. Yao, and L. V. Wang, J. Biomed. Opt. 17, 126019 (2012).
[CrossRef]

Yao, J.

J. Yao and L. V. Wang, Contrast Media Mol. Imag. 6, 332 (2011).
[CrossRef]

J. Yao, K. I. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, J. Biomed. Opt. 16, 076003 (2011).
[CrossRef]

J. Yao, K. I. Maslov, Y. Shi, L. A. Taber, and L. V. Wang, Opt. Lett. 35, 1419 (2010).
[CrossRef]

Zhang, C.

Y. Zhou, C. Zhang, D.-K. Yao, and L. V. Wang, J. Biomed. Opt. 17, 126019 (2012).
[CrossRef]

Zhang, J.

D. Zhu, J. Zhang, H. Cui, Z. Mao, P. Li, and Q. Luo, J. Biomed. Opt. 13, 021106 (2008).
[CrossRef]

Zhang, Y.

J. Yao, K. I. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, J. Biomed. Opt. 16, 076003 (2011).
[CrossRef]

Zhao, Z.

R. Myllylä, Z. Zhao, and M. Kinnunen, in Handbook of Optical Sensing of Glucose in Biological Fluids and TissuesV. V. Tuchin, ed. (Taylor & Francis, 2008).

Zhi, Z.

D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, J. Biomed. Opt. 15, 026008 (2010).
[CrossRef]

Zhou, Y.

Y. Zhou, C. Zhang, D.-K. Yao, and L. V. Wang, J. Biomed. Opt. 17, 126019 (2012).
[CrossRef]

Zhu, D.

J. Wang, R. Shi, and D. Zhu, J. Biomed. Opt. 18, 061209 (2013).
[CrossRef]

D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, J. Biomed. Opt. 15, 026008 (2010).
[CrossRef]

X. Wen, Z. Mao, Z. Han, V. V. Tuchin, and D. Zhu, J. Biophoton. 3, 44 (2010).
[CrossRef]

D. Zhu, J. Zhang, H. Cui, Z. Mao, P. Li, and Q. Luo, J. Biomed. Opt. 13, 021106 (2008).
[CrossRef]

D. Zhu, K. V. Larin, Q. Luo, and V. V. Tuchin, Laser & Photon. Rev. (2013) .

Contrast Media Mol. Imag. (1)

J. Yao and L. V. Wang, Contrast Media Mol. Imag. 6, 332 (2011).
[CrossRef]

J. Biomed. Opt. (5)

Y. Zhou, C. Zhang, D.-K. Yao, and L. V. Wang, J. Biomed. Opt. 17, 126019 (2012).
[CrossRef]

J. Yao, K. I. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, J. Biomed. Opt. 16, 076003 (2011).
[CrossRef]

D. Zhu, J. Zhang, H. Cui, Z. Mao, P. Li, and Q. Luo, J. Biomed. Opt. 13, 021106 (2008).
[CrossRef]

D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, J. Biomed. Opt. 15, 026008 (2010).
[CrossRef]

J. Wang, R. Shi, and D. Zhu, J. Biomed. Opt. 18, 061209 (2013).
[CrossRef]

J. Biophoton. (1)

X. Wen, Z. Mao, Z. Han, V. V. Tuchin, and D. Zhu, J. Biophoton. 3, 44 (2010).
[CrossRef]

J. Phys. D (2)

V. V. Tuchin, J. Phys. D 38, 2497 (2005).
[CrossRef]

M. Kinnunen and R. Myllylä, J. Phys. D 382654 (2005).
[CrossRef]

Opt. Lett. (2)

Photochem. Photobiol. (1)

G. Vargas, A. Readinger, S. S. Dozier, and A. J. Welch, Photochem. Photobiol. 77541 (2003).
[CrossRef]

Science (1)

L. V. Wang and S. Hu, Science 335, 1458 (2012).
[CrossRef]

Other (3)

R. Myllylä, Z. Zhao, and M. Kinnunen, in Handbook of Optical Sensing of Glucose in Biological Fluids and TissuesV. V. Tuchin, ed. (Taylor & Francis, 2008).

L. V. Wang and H. Wu, Biomedical Optics: Principles and Imaging (Wiley, 2007).

D. Zhu, K. V. Larin, Q. Luo, and V. V. Tuchin, Laser & Photon. Rev. (2013) .

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