Abstract

We developed a raster-scan acoustic resolution broadband optoacoustic mesoscopy system and investigated the imaging performance using ultrasonic frequencies up to 125 MHz. The developed system achieves 7 μm axial resolution and transverse resolution of 30 μm reaching depths of at least 5 mm. This unprecedented performance is achieved by operating at out-of-focus ultrasonic detection and tomographic reconstruction. We demonstrate the limits reached due to the width of the laser pulse employed and showcase the technique on drosophila fly and drosophila pupae ex vivo.

© 2013 Optical Society of America

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  1. D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
    [CrossRef]
  2. S. Hu, K. Maslov, and L. V. Wang, Opt. Lett. 36, 1134 (2011).
    [CrossRef]
  3. J. Yao and L. V. Wang, Laser Photon. Rev. (2013).
  4. V. Ntziachristos, Nat. Methods 7, 603 (2010).
    [CrossRef]
  5. A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
    [CrossRef]
  6. C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
    [CrossRef]
  7. G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).
    [CrossRef]
  8. H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
    [CrossRef]
  9. M. Li, H. F. Zhao, K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 31, 474 (2006).
    [CrossRef]
  10. Z. Deng, X. Yang, H. Gong, and Q. Luo, J. Appl. Phys. 109, 104701 (2011).
    [CrossRef]
  11. R. Ma, S. Soentges, S. Shoham, V. Ntziachristos, and D. Razansky, Biomed. Opt. Express 3, 1724 (2012).
    [CrossRef]
  12. M. Á. Araque Caballero, A. Rosenthal, J. Gateau, D. Razansky, and V. Ntziachristos, Opt. Lett. 37, 4080 (2012).
    [CrossRef]
  13. J. Laufer, E. Zhang, G. Raivich, and P. Beard, Appl. Opt. 48, D299 (2009).
    [CrossRef]
  14. M. I. Khan and G. J. Diebold, Ultrasonics 33, 265 (1995).
    [CrossRef]
  15. L. V. Wang and H. Wu, Biomedical Optics: Principles and Imaging (Wiley-Interscience, 2007).
  16. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004).
    [CrossRef]
  17. C. Zhang, K. Maslov, J. Yao, and L. V. Wang, J. Biomed. Opt. 17, 116016 (2012).
    [CrossRef]
  18. X. L. Dean-Ben, D. Razansky, and V. Ntziachristos, Phys. Med. Biol. 56, 6129 (2011).
    [CrossRef]

2013 (1)

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

2012 (3)

2011 (3)

X. L. Dean-Ben, D. Razansky, and V. Ntziachristos, Phys. Med. Biol. 56, 6129 (2011).
[CrossRef]

S. Hu, K. Maslov, and L. V. Wang, Opt. Lett. 36, 1134 (2011).
[CrossRef]

Z. Deng, X. Yang, H. Gong, and Q. Luo, J. Appl. Phys. 109, 104701 (2011).
[CrossRef]

2010 (1)

V. Ntziachristos, Nat. Methods 7, 603 (2010).
[CrossRef]

2009 (2)

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

J. Laufer, E. Zhang, G. Raivich, and P. Beard, Appl. Opt. 48, D299 (2009).
[CrossRef]

2008 (1)

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

2006 (2)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

M. Li, H. F. Zhao, K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 31, 474 (2006).
[CrossRef]

2004 (1)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004).
[CrossRef]

1995 (1)

M. I. Khan and G. J. Diebold, Ultrasonics 33, 265 (1995).
[CrossRef]

1991 (1)

G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).
[CrossRef]

Aichler, M.

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

Araque Caballero, M. Á.

Beard, P.

Beziere, N.

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

Dean-Ben, X. L.

X. L. Dean-Ben, D. Razansky, and V. Ntziachristos, Phys. Med. Biol. 56, 6129 (2011).
[CrossRef]

Del Bene, F.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004).
[CrossRef]

Deng, Z.

Z. Deng, X. Yang, H. Gong, and Q. Luo, J. Appl. Phys. 109, 104701 (2011).
[CrossRef]

Diebold, G. J.

M. I. Khan and G. J. Diebold, Ultrasonics 33, 265 (1995).
[CrossRef]

G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).
[CrossRef]

Distel, M.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

Gateau, J.

Gong, H.

Z. Deng, X. Yang, H. Gong, and Q. Luo, J. Appl. Phys. 109, 104701 (2011).
[CrossRef]

Haag, R.

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

Hu, S.

Huisken, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004).
[CrossRef]

Khan, M. I.

M. I. Khan and G. J. Diebold, Ultrasonics 33, 265 (1995).
[CrossRef]

G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).
[CrossRef]

Kosanke, K.

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

Köster, R. W.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

Laufer, J.

Li, M.

Licha, K.

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

Luo, Q.

Z. Deng, X. Yang, H. Gong, and Q. Luo, J. Appl. Phys. 109, 104701 (2011).
[CrossRef]

Ma, R.

R. Ma, S. Soentges, S. Shoham, V. Ntziachristos, and D. Razansky, Biomed. Opt. Express 3, 1724 (2012).
[CrossRef]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

Maslov, K.

C. Zhang, K. Maslov, J. Yao, and L. V. Wang, J. Biomed. Opt. 17, 116016 (2012).
[CrossRef]

S. Hu, K. Maslov, and L. V. Wang, Opt. Lett. 36, 1134 (2011).
[CrossRef]

M. Li, H. F. Zhao, K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 31, 474 (2006).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Ntziachristos, V.

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

R. Ma, S. Soentges, S. Shoham, V. Ntziachristos, and D. Razansky, Biomed. Opt. Express 3, 1724 (2012).
[CrossRef]

M. Á. Araque Caballero, A. Rosenthal, J. Gateau, D. Razansky, and V. Ntziachristos, Opt. Lett. 37, 4080 (2012).
[CrossRef]

X. L. Dean-Ben, D. Razansky, and V. Ntziachristos, Phys. Med. Biol. 56, 6129 (2011).
[CrossRef]

V. Ntziachristos, Nat. Methods 7, 603 (2010).
[CrossRef]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

Perrimon, N.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

Pitsouli, C.

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

Raivich, G.

Razansky, D.

R. Ma, S. Soentges, S. Shoham, V. Ntziachristos, and D. Razansky, Biomed. Opt. Express 3, 1724 (2012).
[CrossRef]

M. Á. Araque Caballero, A. Rosenthal, J. Gateau, D. Razansky, and V. Ntziachristos, Opt. Lett. 37, 4080 (2012).
[CrossRef]

X. L. Dean-Ben, D. Razansky, and V. Ntziachristos, Phys. Med. Biol. 56, 6129 (2011).
[CrossRef]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

Rosenthal, A.

Rummeny, E.

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

Shoham, S.

Soentges, S.

Stelzer, E. H. K.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004).
[CrossRef]

Stoica, G.

M. Li, H. F. Zhao, K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 31, 474 (2006).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Sun, T.

G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).
[CrossRef]

Swoger, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004).
[CrossRef]

Taruttis, A.

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

Vinegoni, C.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

Walch, A.

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

Wang, L. V.

C. Zhang, K. Maslov, J. Yao, and L. V. Wang, J. Biomed. Opt. 17, 116016 (2012).
[CrossRef]

S. Hu, K. Maslov, and L. V. Wang, Opt. Lett. 36, 1134 (2011).
[CrossRef]

M. Li, H. F. Zhao, K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 31, 474 (2006).
[CrossRef]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

J. Yao and L. V. Wang, Laser Photon. Rev. (2013).

L. V. Wang and H. Wu, Biomedical Optics: Principles and Imaging (Wiley-Interscience, 2007).

Wildgruber, M.

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

Wittbrodt, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004).
[CrossRef]

Wu, H.

L. V. Wang and H. Wu, Biomedical Optics: Principles and Imaging (Wiley-Interscience, 2007).

Yang, X.

Z. Deng, X. Yang, H. Gong, and Q. Luo, J. Appl. Phys. 109, 104701 (2011).
[CrossRef]

Yao, J.

C. Zhang, K. Maslov, J. Yao, and L. V. Wang, J. Biomed. Opt. 17, 116016 (2012).
[CrossRef]

J. Yao and L. V. Wang, Laser Photon. Rev. (2013).

Zhang, C.

C. Zhang, K. Maslov, J. Yao, and L. V. Wang, J. Biomed. Opt. 17, 116016 (2012).
[CrossRef]

Zhang, E.

Zhang, H. F.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Zhao, H. F.

Appl. Opt. (1)

Biomed. Opt. Express (1)

J. Appl. Phys. (1)

Z. Deng, X. Yang, H. Gong, and Q. Luo, J. Appl. Phys. 109, 104701 (2011).
[CrossRef]

J. Biomed. Opt. (1)

C. Zhang, K. Maslov, J. Yao, and L. V. Wang, J. Biomed. Opt. 17, 116016 (2012).
[CrossRef]

Nat. Biotechnol. (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Nat. Methods (2)

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

V. Ntziachristos, Nat. Methods 7, 603 (2010).
[CrossRef]

Nat. Photonics (1)

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Köster, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

Opt. Lett. (3)

Photoacoustics (1)

A. Taruttis, M. Wildgruber, K. Kosanke, N. Beziere, K. Licha, R. Haag, M. Aichler, A. Walch, E. Rummeny, and V. Ntziachristos, Photoacoustics 1, 3 (2013).
[CrossRef]

Phys. Med. Biol. (1)

X. L. Dean-Ben, D. Razansky, and V. Ntziachristos, Phys. Med. Biol. 56, 6129 (2011).
[CrossRef]

Phys. Rev. Lett. (1)

G. J. Diebold, T. Sun, and M. I. Khan, Phys. Rev. Lett. 67, 3384 (1991).
[CrossRef]

Science (1)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004).
[CrossRef]

Ultrasonics (1)

M. I. Khan and G. J. Diebold, Ultrasonics 33, 265 (1995).
[CrossRef]

Other (2)

L. V. Wang and H. Wu, Biomedical Optics: Principles and Imaging (Wiley-Interscience, 2007).

J. Yao and L. V. Wang, Laser Photon. Rev. (2013).

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Figures (3)

Fig. 1.
Fig. 1.

(a) Schematic of the system. (b) Magnitude of the Fourier transform of signals measured for excitations with different laser pulse duration of a 10 μm black microsphere. (c) Time profiles of the signals. (d) Reconstruction of a 10 μm microsphere measured in focus.

Fig. 2.
Fig. 2.

MIP images along the z axis of 100 μm thick slices of a phantom comprised of 10 μm black microspheres: (a) from 400 to 500 μm away from the focus, (b) from 1400 to 1500 μm, (c) from 2400 to 2500 μm. (d) MIP profiles from different depths.

Fig. 3.
Fig. 3.

MIP images along the z axis of (a) an ex vivo drosophila fly, the inset shows a photographic image of the fly; (b) an ex vivo drosophila pupae, both transgenic and expressing GFP in the wings; (c) MIP along the y axis of the pupae; (d) a photographic image of the pupae.

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