Abstract

We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive tool to assess the optical aberrations introduced by the cell. We demonstrate an adaptive optics (AO) system to remove the aberration-related bias in the FFM measurements. Our data show that AO is not only useful when imaging deep in tissues but also when performing FFM measurements through a single cellular layer. This work paves the way for the application of FFM to complex three-dimensional multicellular samples.

© 2013 Optical Society of America

Full Article  |  PDF Article
Related Articles
Spatial fluorescence cross-correlation spectroscopy

Rodolphe Jaffiol, Yoann Blancquaert, Antoine Delon, and Jacques Derouard
Appl. Opt. 45(6) 1225-1235 (2006)

Measuring and imaging diffusion with multiple scan speed image correlation spectroscopy

Nadine Gröner, Jérémie Capoulade, Christoph Cremer, and Malte Wachsmuth
Opt. Express 18(20) 21225-21237 (2010)

Adaptive optics for fluorescence correlation spectroscopy

Charles-Edouard Leroux, Irène Wang, Jacques Derouard, and Antoine Delon
Opt. Express 19(27) 26839-26849 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription