Abstract

An autofocusing method for two-wavelength digital holographic microscopy (TWDHM) based on the wavelength dependence of the diffraction process is proposed. Red and green lights are employed for the illumination of the TWDHM, and the generated holograms are recorded simultaneously by a color CCD camera. Due to the wavelength dependency of the diffraction process, the farther the reconstruction plane is from the image plane, the larger the difference is between the red and green light distributions. Thus, the image plane can be determined by finding the minimum of the variation between the red and green lights on their amplitude distributions. The feasibility of the proposed method is demonstrated by simulation and experiment.

© 2012 Optical Society of America

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  1. E. Cuche, P. Marquet, and C. Depeursinge, Appl. Opt. 39, 4070 (2000).
    [CrossRef]
  2. B. Kemper and G. von Bally, Appl. Opt. 47, A52 (2008).
    [CrossRef]
  3. T. Colomb, F. Montfort, J. Kühn, N. Aspert, E. Cuche, A. Marian, F. Charrière, S. Bourquin, P. Marquet, and C. Depeursinge, J. Opt. Soc. Am. A 23, 3177 (2006).
    [CrossRef]
  4. Y. Awatsuji, M. Sasada, and T. Kubota, Appl. Phys. Lett. 85, 1069 (2004).
    [CrossRef]
  5. P. Langehanenberg, B. Kemper, D. Dirksen, and G. von Bally, Appl. Opt. 47, D176 (2008).
    [CrossRef]
  6. F. Dubois, C. Schockaert, N. Callens, and C. Yourassowsky, Opt. Express 14, 5895 (2006).
    [CrossRef]
  7. M. Antkowiak, N. Callens, C. Yourassowsky, and F. Dubois, Opt. Lett. 33, 1626 (2008).
    [CrossRef]
  8. L. Yu and L. Cai, J. Opt. Soc. Am. A 18, 1033 (2001).
    [CrossRef]
  9. J. Gillespie and R. A. King, Patt. Recog. Lett. 9, 19 (1989).
  10. L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A: Pure Appl. Opt. 6, 396 (2004).
  11. W. Li, N. C. Loomis, Q. Hu, and C. S. Davis, J. Opt. Soc. Am. A 24, 3054 (2007).
    [CrossRef]
  12. M. Liebling and M. Unser, J. Opt. Soc. Am. 21, 2424 (2004).
    [CrossRef]
  13. C. McElhinney, J. McDonald, A. Castro, Y. Frauel, B. Javidi, and T. Naughton, Opt. Lett. 32, 1229 (2007).
    [CrossRef]
  14. P. Ferraro, S. Grilli, D. Alfieri, S. D. Nicola, A. Finizio, G. Pierattini, B. Javidi, G. Coppola, and V. Striano, Opt. Express 13, 6738 (2005).
    [CrossRef]
  15. P. Ferraro, G. Coppola, S. De Nicola, A. Finizio, and G. Pierattini, Opt. Lett. 28, 1257 (2003).
    [CrossRef]
  16. C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
    [CrossRef]
  17. F. C. A. Groen, I. T. Young, and G. Ligthart, Cytometry 6, 81 (1985).
    [CrossRef]
  18. Seungrag Lee, Ji Yong Lee, Wenzhong Yang, and Dug Young Kim, Opt. Express 17, 6476 (2009).
    [CrossRef]
  19. M. Bravo-Zanoguera, B. V. Massenbach, A. L. Kellner, and J. H. Price, Rev. Sci. Instrum. 69, 3966 (1998).
    [CrossRef]
  20. M. T. Özgen and T. E. Tuncer, Opt. Eng. 43, 1300 (2004).
    [CrossRef]
  21. J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Geerts, Cytometry 39, 1 (2000).
    [CrossRef]
  22. M. L. Tachiki, M. Itoh, and T. Yatagai, Appl. Opt. 47, D144 (2008).
    [CrossRef]
  23. D. Lebrun, A. Benkouider, S. Coëtmellec, and M. Malek, Opt. Express 11, 224 (2003).
    [CrossRef]
  24. Y. Park, G. Popescu, K. Badizadegan, R. Dasari, and M. Feld, Opt. Lett. 32, 811 (2007).
    [CrossRef]
  25. J. Sheng, E. Malkiel, and J. Katz, Appl. Opt. 45, 3893 (2006).
    [CrossRef]
  26. B. Javidi, S. Yeom, I. Moon, and M. Daneshpanah, Opt. Express 14, 3806 (2006).
    [CrossRef]
  27. J. Kühn, T. Colomb, F. Montfort, F. Charrière, Y. Emery, E. Cuche, P. Marquet, and C. Depeursinge, Opt. Express 15, 7231 (2007).
    [CrossRef]
  28. P. Ferraro, L. Miccio, S. Grilli, M. Paturzo, S. De Nicola, A. Finizio, R. Osellame, and P. Laporta, Opt. Express 15, 14591 (2007).
    [CrossRef]

2009 (1)

2008 (4)

2007 (5)

2006 (4)

2005 (1)

2004 (4)

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A: Pure Appl. Opt. 6, 396 (2004).

M. Liebling and M. Unser, J. Opt. Soc. Am. 21, 2424 (2004).
[CrossRef]

Y. Awatsuji, M. Sasada, and T. Kubota, Appl. Phys. Lett. 85, 1069 (2004).
[CrossRef]

M. T. Özgen and T. E. Tuncer, Opt. Eng. 43, 1300 (2004).
[CrossRef]

2003 (2)

2001 (1)

2000 (3)

J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Geerts, Cytometry 39, 1 (2000).
[CrossRef]

E. Cuche, P. Marquet, and C. Depeursinge, Appl. Opt. 39, 4070 (2000).
[CrossRef]

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

1998 (1)

M. Bravo-Zanoguera, B. V. Massenbach, A. L. Kellner, and J. H. Price, Rev. Sci. Instrum. 69, 3966 (1998).
[CrossRef]

1989 (1)

J. Gillespie and R. A. King, Patt. Recog. Lett. 9, 19 (1989).

1985 (1)

F. C. A. Groen, I. T. Young, and G. Ligthart, Cytometry 6, 81 (1985).
[CrossRef]

Alfieri, D.

Antkowiak, M.

Aspert, N.

Awatsuji, Y.

Y. Awatsuji, M. Sasada, and T. Kubota, Appl. Phys. Lett. 85, 1069 (2004).
[CrossRef]

Badizadegan, K.

Benkouider, A.

Bourquin, S.

Bravo-Zanoguera, M.

M. Bravo-Zanoguera, B. V. Massenbach, A. L. Kellner, and J. H. Price, Rev. Sci. Instrum. 69, 3966 (1998).
[CrossRef]

Buraga-Lefebvre, C.

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

Cai, L.

Callens, N.

Castro, A.

Charrière, F.

Coëtmellec, S.

D. Lebrun, A. Benkouider, S. Coëtmellec, and M. Malek, Opt. Express 11, 224 (2003).
[CrossRef]

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

Colomb, T.

Coppola, G.

Cornelissen, F.

J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Geerts, Cytometry 39, 1 (2000).
[CrossRef]

Cuche, E.

Daneshpanah, M.

Dasari, R.

Davis, C. S.

De Nicola, S.

Depeursinge, C.

Dirksen, D.

Dubois, F.

Emery, Y.

Feld, M.

Ferraro, P.

Finizio, A.

Frauel, Y.

Geerts, H.

J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Geerts, Cytometry 39, 1 (2000).
[CrossRef]

Geusebroek, J.-M.

J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Geerts, Cytometry 39, 1 (2000).
[CrossRef]

Gillespie, J.

J. Gillespie and R. A. King, Patt. Recog. Lett. 9, 19 (1989).

Grilli, S.

Groen, F. C. A.

F. C. A. Groen, I. T. Young, and G. Ligthart, Cytometry 6, 81 (1985).
[CrossRef]

Hu, Q.

Itoh, M.

Javidi, B.

Jin, H.

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A: Pure Appl. Opt. 6, 396 (2004).

Katz, J.

Kellner, A. L.

M. Bravo-Zanoguera, B. V. Massenbach, A. L. Kellner, and J. H. Price, Rev. Sci. Instrum. 69, 3966 (1998).
[CrossRef]

Kemper, B.

Kim, Dug Young

King, R. A.

J. Gillespie and R. A. King, Patt. Recog. Lett. 9, 19 (1989).

Kubota, T.

Y. Awatsuji, M. Sasada, and T. Kubota, Appl. Phys. Lett. 85, 1069 (2004).
[CrossRef]

Kühn, J.

Langehanenberg, P.

Laporta, P.

Lebrun, D.

D. Lebrun, A. Benkouider, S. Coëtmellec, and M. Malek, Opt. Express 11, 224 (2003).
[CrossRef]

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

Lee, Ji Yong

Lee, Seungrag

Li, W.

Li, Y.

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A: Pure Appl. Opt. 6, 396 (2004).

Liebling, M.

M. Liebling and M. Unser, J. Opt. Soc. Am. 21, 2424 (2004).
[CrossRef]

Ligthart, G.

F. C. A. Groen, I. T. Young, and G. Ligthart, Cytometry 6, 81 (1985).
[CrossRef]

Loomis, N. C.

Ma, L.

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A: Pure Appl. Opt. 6, 396 (2004).

Malek, M.

Malkiel, E.

Marian, A.

Marquet, P.

Massenbach, B. V.

M. Bravo-Zanoguera, B. V. Massenbach, A. L. Kellner, and J. H. Price, Rev. Sci. Instrum. 69, 3966 (1998).
[CrossRef]

McDonald, J.

McElhinney, C.

Miccio, L.

Montfort, F.

Moon, I.

Naughton, T.

Nicola, S. D.

Osellame, R.

Özgen, M. T.

M. T. Özgen and T. E. Tuncer, Opt. Eng. 43, 1300 (2004).
[CrossRef]

Özkul, C.

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

Park, Y.

Paturzo, M.

Pierattini, G.

Popescu, G.

Price, J. H.

M. Bravo-Zanoguera, B. V. Massenbach, A. L. Kellner, and J. H. Price, Rev. Sci. Instrum. 69, 3966 (1998).
[CrossRef]

Sasada, M.

Y. Awatsuji, M. Sasada, and T. Kubota, Appl. Phys. Lett. 85, 1069 (2004).
[CrossRef]

Schockaert, C.

Sheng, J.

Smeulders, A. W. M.

J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Geerts, Cytometry 39, 1 (2000).
[CrossRef]

Striano, V.

Tachiki, M. L.

Tuncer, T. E.

M. T. Özgen and T. E. Tuncer, Opt. Eng. 43, 1300 (2004).
[CrossRef]

Unser, M.

M. Liebling and M. Unser, J. Opt. Soc. Am. 21, 2424 (2004).
[CrossRef]

von Bally, G.

Wang, H.

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A: Pure Appl. Opt. 6, 396 (2004).

Yang, Wenzhong

Yatagai, T.

Yeom, S.

Young, I. T.

F. C. A. Groen, I. T. Young, and G. Ligthart, Cytometry 6, 81 (1985).
[CrossRef]

Yourassowsky, C.

Yu, L.

Appl. Opt. (5)

Appl. Phys. Lett. (1)

Y. Awatsuji, M. Sasada, and T. Kubota, Appl. Phys. Lett. 85, 1069 (2004).
[CrossRef]

Cytometry (2)

F. C. A. Groen, I. T. Young, and G. Ligthart, Cytometry 6, 81 (1985).
[CrossRef]

J.-M. Geusebroek, F. Cornelissen, A. W. M. Smeulders, and H. Geerts, Cytometry 39, 1 (2000).
[CrossRef]

J. Opt. A: Pure Appl. Opt. (1)

L. Ma, H. Wang, Y. Li, and H. Jin, J. Opt. A: Pure Appl. Opt. 6, 396 (2004).

J. Opt. Soc. Am. (1)

M. Liebling and M. Unser, J. Opt. Soc. Am. 21, 2424 (2004).
[CrossRef]

J. Opt. Soc. Am. A (3)

Opt. Eng. (1)

M. T. Özgen and T. E. Tuncer, Opt. Eng. 43, 1300 (2004).
[CrossRef]

Opt. Express (7)

Opt. Lasers Eng. (1)

C. Buraga-Lefebvre, S. Coëtmellec, D. Lebrun, and C. Özkul, Opt. Lasers Eng. 33, 409 (2000).
[CrossRef]

Opt. Lett. (4)

Patt. Recog. Lett. (1)

J. Gillespie and R. A. King, Patt. Recog. Lett. 9, 19 (1989).

Rev. Sci. Instrum. (1)

M. Bravo-Zanoguera, B. V. Massenbach, A. L. Kellner, and J. H. Price, Rev. Sci. Instrum. 69, 3966 (1998).
[CrossRef]

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Figures (4)

Fig. 1.
Fig. 1.

Simulation results for autofocusing of TWDHM. (a) and (b) amplitude and phase distributions of the simulated specimen for red light; (c) computed two-color hologram comprised of Ir(x,y) and Ig(x,y); (d) Ag/Ag0Ar/Ar0 in the hologram plane with Δz=0; (e) and (f) focus criteria versus refocusing distance Δz.

Fig. 2.
Fig. 2.

Experimental setup. NF1-NF3: variable neutral attenuator; MO, microscope objective (Mitutoyo M Plan Apo, 50X/0.55); L: achromatic doublet lens with focal length fL=175mm.

Fig. 3.
Fig. 3.

Experimental results on TWDHM autofocusing for a microscopic rectangular phase-step. (a)–(c), Ag/Ag0Ar/Ar0 in planes of Δz=0cm, 7.5 cm, and 15 cm, respectively; (d) focus criterion versus refocusing distance Δz; (e) reconstructed phase distribution of the specimen for red light with Δz=15mm.

Fig. 4.
Fig. 4.

Experimental results on a microscopic ruler and PMMA beads; hologram (a) and focus criteria (b) of the microscopic ruler; hologram (c) and focus criteria (d) of the PMMA beads.

Equations (2)

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Or(x,y,Δz)=IFT{FT{IrRr}·Wr·exp[ikrΔz1(λrξ)2(λrη)2]}.
Cri(Δz)=1NxNyx=1Nxy=1Ny(uu¯)2,

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