Abstract

This study utilizes Monte Carlo simulations of single fiber fluorescence to develop an empirical model that corrects for the influence of scattering and absorption on fluorescence intensity (FSF). The model expresses FSF in terms of the reduced scattering coefficient (μs) and absorption coefficient (μa), each determined independently at excitation and emission wavelengths (λx and λm), and the fiber diameter (df). This model returns accurate descriptions (mean residual <6%) of FSF across a biologically relevant range of μs and μa values and is insensitive to the form of the scattering phase function.

© 2012 Optical Society of America

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2012 (1)

2011 (2)

2007 (1)

H. Stepp, T. Beck, W. Beyer, C. Pfaller, M. Schuppler, R. Sroka, and R. Baumgartner, Med. Laser Appl. 22, 23 (2007).
[CrossRef]

2003 (1)

2001 (2)

M. G. Müller, I. Georgakoudi, Q. Zhang, J. Wu, and M. S. Feld, Appl. Opt. 40, 4633 (2001).
[CrossRef]

T. J. Pfefer, K. T. Schomacker, M. N. Ediger, and N. S. Nishioka, IEEE J. Sel. Top. Quantum Electron. 7, 1004 (2001).
[CrossRef]

1999 (1)

1997 (1)

A. J. Welch, C. Gardner, R. Richards-Kortum, E. Chan, G. Criswell, J. Pfefer, and S. Warren, Lasers Surg. Med. 21, 166 (1997).
[CrossRef]

1996 (1)

1993 (1)

Amelink, A.

Baumgartner, R.

H. Stepp, T. Beck, W. Beyer, C. Pfaller, M. Schuppler, R. Sroka, and R. Baumgartner, Med. Laser Appl. 22, 23 (2007).
[CrossRef]

Beck, T.

H. Stepp, T. Beck, W. Beyer, C. Pfaller, M. Schuppler, R. Sroka, and R. Baumgartner, Med. Laser Appl. 22, 23 (2007).
[CrossRef]

Bevilacqua, F.

Beyer, W.

H. Stepp, T. Beck, W. Beyer, C. Pfaller, M. Schuppler, R. Sroka, and R. Baumgartner, Med. Laser Appl. 22, 23 (2007).
[CrossRef]

Chan, E.

A. J. Welch, C. Gardner, R. Richards-Kortum, E. Chan, G. Criswell, J. Pfefer, and S. Warren, Lasers Surg. Med. 21, 166 (1997).
[CrossRef]

Criswell, G.

A. J. Welch, C. Gardner, R. Richards-Kortum, E. Chan, G. Criswell, J. Pfefer, and S. Warren, Lasers Surg. Med. 21, 166 (1997).
[CrossRef]

Depeursinge, C.

Diamond, K. R.

Ediger, M. N.

T. J. Pfefer, K. T. Schomacker, M. N. Ediger, and N. S. Nishioka, IEEE J. Sel. Top. Quantum Electron. 7, 1004 (2001).
[CrossRef]

Farrell, T. J.

Feld, M. S.

Gamm, U. A.

Gardner, C.

A. J. Welch, C. Gardner, R. Richards-Kortum, E. Chan, G. Criswell, J. Pfefer, and S. Warren, Lasers Surg. Med. 21, 166 (1997).
[CrossRef]

Gardner, C. M.

Georgakoudi, I.

Jacques, S. L.

Kanick, S. C.

Müller, M. G.

Nishioka, N. S.

T. J. Pfefer, K. T. Schomacker, M. N. Ediger, and N. S. Nishioka, IEEE J. Sel. Top. Quantum Electron. 7, 1004 (2001).
[CrossRef]

Patterson, M. S.

Pfaller, C.

H. Stepp, T. Beck, W. Beyer, C. Pfaller, M. Schuppler, R. Sroka, and R. Baumgartner, Med. Laser Appl. 22, 23 (2007).
[CrossRef]

Pfefer, J.

A. J. Welch, C. Gardner, R. Richards-Kortum, E. Chan, G. Criswell, J. Pfefer, and S. Warren, Lasers Surg. Med. 21, 166 (1997).
[CrossRef]

Pfefer, T. J.

T. J. Pfefer, K. T. Schomacker, M. N. Ediger, and N. S. Nishioka, IEEE J. Sel. Top. Quantum Electron. 7, 1004 (2001).
[CrossRef]

Rava, R. P.

Richards-Kortum, R.

A. J. Welch, C. Gardner, R. Richards-Kortum, E. Chan, G. Criswell, J. Pfefer, and S. Warren, Lasers Surg. Med. 21, 166 (1997).
[CrossRef]

Robinson, D. J.

Schomacker, K. T.

T. J. Pfefer, K. T. Schomacker, M. N. Ediger, and N. S. Nishioka, IEEE J. Sel. Top. Quantum Electron. 7, 1004 (2001).
[CrossRef]

Schuppler, M.

H. Stepp, T. Beck, W. Beyer, C. Pfaller, M. Schuppler, R. Sroka, and R. Baumgartner, Med. Laser Appl. 22, 23 (2007).
[CrossRef]

Sroka, R.

H. Stepp, T. Beck, W. Beyer, C. Pfaller, M. Schuppler, R. Sroka, and R. Baumgartner, Med. Laser Appl. 22, 23 (2007).
[CrossRef]

Stepp, H.

H. Stepp, T. Beck, W. Beyer, C. Pfaller, M. Schuppler, R. Sroka, and R. Baumgartner, Med. Laser Appl. 22, 23 (2007).
[CrossRef]

Sterenborg, H. J. C. M.

Warren, S.

A. J. Welch, C. Gardner, R. Richards-Kortum, E. Chan, G. Criswell, J. Pfefer, and S. Warren, Lasers Surg. Med. 21, 166 (1997).
[CrossRef]

Welch, A. J.

A. J. Welch, C. Gardner, R. Richards-Kortum, E. Chan, G. Criswell, J. Pfefer, and S. Warren, Lasers Surg. Med. 21, 166 (1997).
[CrossRef]

C. M. Gardner, S. L. Jacques, and A. J. Welch, Appl. Opt. 35, 1780 (1996).
[CrossRef]

Wu, J.

Zhang, Q.

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Figures (4)

Fig. 1.
Fig. 1.

Dimensionless FSF° versus dimensionless reduced scattering coefficient on (A) linear and (B) log scales. Colors indicate different fiber diameters.

Fig. 2.
Fig. 2.

Dimensionless FSF versus dimensionless reduced scattering coefficient on (A) linear and (B) log scales. Colors indicate different fiber diameters, as in Fig. 1.

Fig. 3.
Fig. 3.

Ratio of SF fluorescence measured with and without background absorption, plotted versus dimensionless absorption on (A) linear and (B) log scales.

Fig. 4.
Fig. 4.

(A) Dimensionless FSF estimated by mathematical model versus simulated by MC model. (B) Residual between model and simulated dimensionless fluorescence.

Equations (5)

Equations on this page are rendered with MathJax. Learn more.

FSF°μafQfϕxVHmVV,
FSF°μafQfdfνn=ζ1(μs,avgdf)ζ2e(1ζ2(μs(λx)df)+1ζ3ζ2(μs(λm)df)+1),
FSF=FSF°e(μa,avgLSFFL),
LSFFLGeneral=Z1+C1(μs(λx)df)C2+C3(μs(λm)df)C41+C5(μa(λx)df)C6+C7(μa(λm)df)C8,
LSFFL=Z1+κ(μs,avgdf)1+(μa,avgdf),

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