Abstract

This study utilizes Monte Carlo simulations of single fiber fluorescence to develop an empirical model that corrects for the influence of scattering and absorption on fluorescence intensity (FSF). The model expresses FSF in terms of the reduced scattering coefficient (μs) and absorption coefficient (μa), each determined independently at excitation and emission wavelengths (λx and λm), and the fiber diameter (df). This model returns accurate descriptions (mean residual <6%) of FSF across a biologically relevant range of μs and μa values and is insensitive to the form of the scattering phase function.

© 2012 Optical Society of America

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