Abstract

Quantification of intracellular nanoscale macromolecular density distribution is a fundamental aspect to understanding cellular processes. We report a near-field penetrating optical microscopy (NPOM) technique to directly probe the internal nanoscale macromolecular density of biological cells through quantification of intracellular refractive index (RI). NPOM inserts a tapered optical fiber probe to successive depths into an illuminated sample. A 50nm diameter probe tip collects signal that exhibits a linear relationship with the sample RI at a spatial resolution of approximately 50nm for biologically relevant measurements, one order of magnitude finer than the Abbe diffraction limit. Live and fixed cell data illustrate the mechanical ability of a 50nm probe to penetrate biological samples.

© 2012 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Modulation transfer function evaluation of linear solid-state x-ray-sensitive detectors using edge techniques

Yue Min Zhu, Valérie Kaftandjian, Gilles Peix, and Daniel Babot
Appl. Opt. 34(22) 4937-4943 (1995)

Observation of the dynamics of live cardiomyocytes through a free-running scanning near-field optical microscopy setup

Ruggero Micheletto, Morgan Denyer, Martin Scholl, Ken Nakajima, Andreas Offenhauser, Masahiko Hara, and Wolfgang Knoll
Appl. Opt. 38(31) 6648-6652 (1999)

Resolving single fluorophores within dense ensembles: contrast limits of tip-enhanced fluorescence microscopy

Benjamin D. Mangum, Chun Mu, and Jordan M. Gerton
Opt. Express 16(9) 6183-6193 (2008)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription