Abstract

We demonstrate a technique of hyperspectral imaging in stimulated Raman scattering (SRS) microscopy using a tunable optical filter, whose transmission wavelength can be varied quickly by a galvanometer mirror. Experimentally, broadband Yb fiber laser pulses are synchronized with picosecond Ti:sapphire pulses, and then spectrally filtered out by the filter. After amplification by fiber amplifiers, we obtain narrowband pulses with a spectral width of <3.3cm1 and a wavelength tunability of >225cm1. By using these pulses, we accomplish SRS imaging of polymer beads with spectral information.

© 2012 Optical Society of America

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  1. C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
    [CrossRef]
  2. Y. Ozeki, F. Dake, S. Kajiyama, K. Fukui, and K. Itoh, Opt. Express 17, 3651 (2009).
    [CrossRef]
  3. P. Nandakumar, A. Kovalev, and A. Volkmer, New J. Phys. 11, 033026 (2009).
    [CrossRef]
  4. B. G. Saar, C. W. Freudiger, J. Reichman, C. M. Stanley, G. R. Holtom, and X. S. Xie, Science 330, 1368 (2010).
    [CrossRef]
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    [CrossRef]
  6. H. T. Beier, G. D. Noojin, and B. A. Rockwell, Opt. Express 19, 18885 (2011).
    [CrossRef]
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    [CrossRef]
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    [CrossRef]

2011 (2)

2010 (3)

2009 (2)

Y. Ozeki, F. Dake, S. Kajiyama, K. Fukui, and K. Itoh, Opt. Express 17, 3651 (2009).
[CrossRef]

P. Nandakumar, A. Kovalev, and A. Volkmer, New J. Phys. 11, 033026 (2009).
[CrossRef]

2008 (1)

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

Andresen, E. R.

Beier, H. T.

Berto, P.

Cerullo, G.

Dake, F.

Freudiger, C. W.

B. G. Saar, C. W. Freudiger, J. Reichman, C. M. Stanley, G. R. Holtom, and X. S. Xie, Science 330, 1368 (2010).
[CrossRef]

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

Fukui, K.

Gambetta, A.

Grancini, G.

He, C.

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

Holtom, G. R.

B. G. Saar, C. W. Freudiger, J. Reichman, C. M. Stanley, G. R. Holtom, and X. S. Xie, Science 330, 1368 (2010).
[CrossRef]

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

Itoh, K.

Kajiyama, S.

Kang, J. X.

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

Kitagawa, Y.

Kovalev, A.

P. Nandakumar, A. Kovalev, and A. Volkmer, New J. Phys. 11, 033026 (2009).
[CrossRef]

Kumar, V.

Lu, S.

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

Marangoni, M.

Min, W.

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

Nandakumar, P.

P. Nandakumar, A. Kovalev, and A. Volkmer, New J. Phys. 11, 033026 (2009).
[CrossRef]

Nishizawa, N.

Noojin, G. D.

Ozeki, Y.

Polli, D.

Ramponi, R.

Reichman, J.

B. G. Saar, C. W. Freudiger, J. Reichman, C. M. Stanley, G. R. Holtom, and X. S. Xie, Science 330, 1368 (2010).
[CrossRef]

Rigneault, H.

Rockwell, B. A.

Saar, B. G.

B. G. Saar, C. W. Freudiger, J. Reichman, C. M. Stanley, G. R. Holtom, and X. S. Xie, Science 330, 1368 (2010).
[CrossRef]

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

Stanley, C. M.

B. G. Saar, C. W. Freudiger, J. Reichman, C. M. Stanley, G. R. Holtom, and X. S. Xie, Science 330, 1368 (2010).
[CrossRef]

Sumimura, K.

Tsai, J. C.

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

Umemura, W.

Volkmer, A.

P. Nandakumar, A. Kovalev, and A. Volkmer, New J. Phys. 11, 033026 (2009).
[CrossRef]

Xie, X. S.

B. G. Saar, C. W. Freudiger, J. Reichman, C. M. Stanley, G. R. Holtom, and X. S. Xie, Science 330, 1368 (2010).
[CrossRef]

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

New J. Phys. (1)

P. Nandakumar, A. Kovalev, and A. Volkmer, New J. Phys. 11, 033026 (2009).
[CrossRef]

Opt. Express (3)

Opt. Lett. (2)

Science (2)

C. W. Freudiger, W. Min, B. G. Saar, S. Lu, G. R. Holtom, C. He, J. C. Tsai, J. X. Kang, and X. S. Xie, Science 322, 1857 (2008).
[CrossRef]

B. G. Saar, C. W. Freudiger, J. Reichman, C. M. Stanley, G. R. Holtom, and X. S. Xie, Science 330, 1368 (2010).
[CrossRef]

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Figures (4)

Fig. 1.
Fig. 1.

Schematic of the TBPF. f1,50 mm;f2, 100 mm; G, reflective grating with a density of 1200lines/mm; M, mirror.

Fig. 2.
Fig. 2.

Experimental setup. PBS, polarization beam splitter; YDFA, Yb-doped fiber amplifier; P, polarizer; DM, dichroic mirror; TPA-PD, two-photon absorption photodiode; OB, objective lens; F, short-pass filter.

Fig. 3.
Fig. 3.

Solid curves: spectra of wavelength-tunable pulses. White circle: spectral width of pulses.

Fig. 4.
Fig. 4.

Results of hyperspectral imaging of PS beads and PMMA beads. Pixel dwell time, 20 µs; number of pixels, 101×101; image size, 40μm×40μm; scale bar, 10 µm; wavenumber step size, 2cm1. (a)–(d) SRS images of different wavenumbers. (e) SRS spectra of PS and PMMA reconstructed from the SRS images.

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