Abstract

Digital holographic microscopy (DHM) is one of the most effective techniques used for quantitative phase imaging of cells. Here we present a compact, easy to implement, portable, and very stable DHM setup employing a self-referencing Lloyd’s mirror configuration. The microscope is constructed using a diode laser source and a CMOS sensor, making it cost effective. The reconstruction of recorded holograms yields the amplitude and phase information of the object. The temporal stability of the presented technique was found to be around 0.9 nm without any vibration compensation, which makes it ideal for studying cell profile changes. This aspect of the technique is demonstrated by studying membrane fluctuations of red blood cells.

© 2012 Optical Society of America

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References

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2011 (3)

M. Mir, K. Tangella, and G. Popescu, Biomed. Opt. Express 2, 3259 (2011).
[CrossRef]

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef]

A. Anand, V. K. Chhaniwal, and B. Javidi, IEEE Photon. J. 3, 546 (2011).
[CrossRef]

2010 (3)

2009 (2)

2008 (1)

2006 (1)

G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 11, 040503 (2006).
[CrossRef]

2005 (1)

2003 (1)

2002 (1)

1998 (1)

1967 (1)

Anand, A.

A. Anand, V. K. Chhaniwal, N. R. Patel, and B. Javidi, IEEE Photon. J. 4, 1456 (2012).
[CrossRef]

A. S. G. Singh, A. Anand, R. A. Leitgeb, and B. Javidi, Opt. Express 20, 23617 (2012).
[CrossRef]

A. Anand, V. K. Chhaniwal, and B. Javidi, IEEE Photon. J. 3, 546 (2011).
[CrossRef]

Badizadegan, K.

G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 11, 040503 (2006).
[CrossRef]

Bae, C. Y.

Bon, P.

Chhaniwal, V. K.

A. Anand, V. K. Chhaniwal, N. R. Patel, and B. Javidi, IEEE Photon. J. 4, 1456 (2012).
[CrossRef]

A. Anand, V. K. Chhaniwal, and B. Javidi, IEEE Photon. J. 3, 546 (2011).
[CrossRef]

Colomb, T.

Coppola, G.

Cuche, E.

Dasari, R. R.

G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 11, 040503 (2006).
[CrossRef]

de Boor, J.

De Nicola, S.

Depeursinge, C.

Dirksen, D.

Dubois, F.

Eigenthaler, U.

Emery, Y.

Faridian, A.

Feld, M. S.

G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 11, 040503 (2006).
[CrossRef]

Ferrari, J. A.

Ferraro, P.

Finizio, A.

Frins, E. M.

Hammer, M.

Hirscher, M.

Hopp, D.

Jang, J.

Javidi, B.

A. S. G. Singh, A. Anand, R. A. Leitgeb, and B. Javidi, Opt. Express 20, 23617 (2012).
[CrossRef]

A. Anand, V. K. Chhaniwal, N. R. Patel, and B. Javidi, IEEE Photon. J. 4, 1456 (2012).
[CrossRef]

A. Anand, V. K. Chhaniwal, and B. Javidi, IEEE Photon. J. 3, 546 (2011).
[CrossRef]

Kemper, B.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef]

P. Langehanenberg, B. Kemper, D. Dirksen, and G. von Bally, Appl. Opt. 47, D176 (2008).
[CrossRef]

Kim, D. K.

Kolb, A.

Langehanenberg, P.

Langenbeck, P. H.

Leitgeb, R. A.

Magistretti, P. J.

Marquet, P.

Maucort, G.

Michel, B.

Mir, M.

Monneret, S.

Murphy, D. B.

D. B. Murphy, Fundamentals of Light Microscopy and Electronic Imaging (Wiley–Liss, 2001).

Osten, W.

Park, J.-K.

Patel, N. R.

A. Anand, V. K. Chhaniwal, N. R. Patel, and B. Javidi, IEEE Photon. J. 4, 1456 (2012).
[CrossRef]

Pedrini, G.

Pierattini, G.

Popescu, G.

M. Mir, K. Tangella, and G. Popescu, Biomed. Opt. Express 2, 3259 (2011).
[CrossRef]

G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 11, 040503 (2006).
[CrossRef]

Rappaz, B.

Rinehart, M. T.

Rommel, C. E.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef]

Schmidt, V.

Schnekenburger, J.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef]

Schweitzer, D.

Shaked, N. T.

Singh, A. S. G.

Tangella, K.

Thamm, E.

Vollmer, A.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef]

von Bally, G.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef]

P. Langehanenberg, B. Kemper, D. Dirksen, and G. von Bally, Appl. Opt. 47, D176 (2008).
[CrossRef]

Wattellier, B.

Wax, A.

Ye, J. C.

Yourassowsky, C.

Appl. Opt. (4)

Biomed. Opt. Express (1)

IEEE Photon. J. (2)

A. Anand, V. K. Chhaniwal, and B. Javidi, IEEE Photon. J. 3, 546 (2011).
[CrossRef]

A. Anand, V. K. Chhaniwal, N. R. Patel, and B. Javidi, IEEE Photon. J. 4, 1456 (2012).
[CrossRef]

J. Biomed. Opt. (2)

G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 11, 040503 (2006).
[CrossRef]

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[CrossRef]

Opt. Express (3)

Opt. Lett. (7)

Other (1)

D. B. Murphy, Fundamentals of Light Microscopy and Electronic Imaging (Wiley–Liss, 2001).

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Figures (4)

Fig. 1.
Fig. 1.

Experimental setup of self-referencing DHM using Lloyd’s mirror configuration.

Fig. 2.
Fig. 2.

(a) Part of the recorded hologram, (b) obtained wrapped phase distribution after phase subtraction, and (c) computed thickness distribution.

Fig. 3
Fig. 3

(a) Histogram of standard deviations of optical path-length variations and (b) OPL variation at the center of the measured phase distribution.

Fig. 4.
Fig. 4.

(a) Phase-contrast image of the analyzed RBC, (b) mean fluctuation map of the cell marked by the rectangle, and (c) temporal evolution of cell thickness at points shown in (a). Standard deviations of the fluctuations are also shown.

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