Abstract

Membrane permeabilization is imperative for gene and drug delivery systems, along with other cell manipulation methods, since the average eukaryotic cell membrane is not permeable to polar and large nonpolar molecules. Antibody conjugated optically absorbing gold nanospheres are targeted to the cell membrane of T47D breast cancer cell line and irradiated with 5 ns pulse, 20 Hz, 532 nm light to increase membrane permeability. Up to 90% permeabilization with less than 6% death is reported at radiant exposures up to 10 times lower than those of other comparable studies.

© 2012 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Principles of Membrane Transport, 4th ed.(Garland Science, 2002).
  2. D. J. Stevenson, F. J. Gunn-Moore, P. Campbell, and K. Dholakia, J. R. Soc. Interface 7, 863 (2010).
    [CrossRef]
  3. P. Chakravarty, W. Qian, M. A. El-Sayed, and M. R. Prausnitz, Nat. Nanotechnol. 5, 607 (2010).
    [CrossRef]
  4. C. Yao, X. Qu, Z. Zhang, G. Hüttmann, and R. Rahmanzadeh, J. Biomed. Opt. 14, 054034 (2009).
    [CrossRef]
  5. E. Sassaroli, K. C. P. Li, and B. E. O’Neill, Phys. Med. Biol. 54, 5541 (2009).
    [CrossRef]
  6. K. Bhattacharyya, B. S. Goldschmidt, M. Hannink, S. Alexander, A. Jurkevic, and J. A. Viator, Clin. Lab. Med. 32, 89 (2012).
    [CrossRef]
  7. J. Gehl, Acta Physiol. Scand. 177, 437 (2003).
    [CrossRef]

2012 (1)

K. Bhattacharyya, B. S. Goldschmidt, M. Hannink, S. Alexander, A. Jurkevic, and J. A. Viator, Clin. Lab. Med. 32, 89 (2012).
[CrossRef]

2010 (2)

D. J. Stevenson, F. J. Gunn-Moore, P. Campbell, and K. Dholakia, J. R. Soc. Interface 7, 863 (2010).
[CrossRef]

P. Chakravarty, W. Qian, M. A. El-Sayed, and M. R. Prausnitz, Nat. Nanotechnol. 5, 607 (2010).
[CrossRef]

2009 (2)

E. Sassaroli, K. C. P. Li, and B. E. O’Neill, Phys. Med. Biol. 54, 5541 (2009).
[CrossRef]

C. Yao, X. Qu, Z. Zhang, G. Hüttmann, and R. Rahmanzadeh, J. Biomed. Opt. 14, 054034 (2009).
[CrossRef]

2003 (1)

J. Gehl, Acta Physiol. Scand. 177, 437 (2003).
[CrossRef]

Alberts, B.

B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Principles of Membrane Transport, 4th ed.(Garland Science, 2002).

Alexander, S.

K. Bhattacharyya, B. S. Goldschmidt, M. Hannink, S. Alexander, A. Jurkevic, and J. A. Viator, Clin. Lab. Med. 32, 89 (2012).
[CrossRef]

Bhattacharyya, K.

K. Bhattacharyya, B. S. Goldschmidt, M. Hannink, S. Alexander, A. Jurkevic, and J. A. Viator, Clin. Lab. Med. 32, 89 (2012).
[CrossRef]

Campbell, P.

D. J. Stevenson, F. J. Gunn-Moore, P. Campbell, and K. Dholakia, J. R. Soc. Interface 7, 863 (2010).
[CrossRef]

Chakravarty, P.

P. Chakravarty, W. Qian, M. A. El-Sayed, and M. R. Prausnitz, Nat. Nanotechnol. 5, 607 (2010).
[CrossRef]

Dholakia, K.

D. J. Stevenson, F. J. Gunn-Moore, P. Campbell, and K. Dholakia, J. R. Soc. Interface 7, 863 (2010).
[CrossRef]

El-Sayed, M. A.

P. Chakravarty, W. Qian, M. A. El-Sayed, and M. R. Prausnitz, Nat. Nanotechnol. 5, 607 (2010).
[CrossRef]

Gehl, J.

J. Gehl, Acta Physiol. Scand. 177, 437 (2003).
[CrossRef]

Goldschmidt, B. S.

K. Bhattacharyya, B. S. Goldschmidt, M. Hannink, S. Alexander, A. Jurkevic, and J. A. Viator, Clin. Lab. Med. 32, 89 (2012).
[CrossRef]

Gunn-Moore, F. J.

D. J. Stevenson, F. J. Gunn-Moore, P. Campbell, and K. Dholakia, J. R. Soc. Interface 7, 863 (2010).
[CrossRef]

Hannink, M.

K. Bhattacharyya, B. S. Goldschmidt, M. Hannink, S. Alexander, A. Jurkevic, and J. A. Viator, Clin. Lab. Med. 32, 89 (2012).
[CrossRef]

Hüttmann, G.

C. Yao, X. Qu, Z. Zhang, G. Hüttmann, and R. Rahmanzadeh, J. Biomed. Opt. 14, 054034 (2009).
[CrossRef]

Johnson, A.

B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Principles of Membrane Transport, 4th ed.(Garland Science, 2002).

Jurkevic, A.

K. Bhattacharyya, B. S. Goldschmidt, M. Hannink, S. Alexander, A. Jurkevic, and J. A. Viator, Clin. Lab. Med. 32, 89 (2012).
[CrossRef]

Lewis, J.

B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Principles of Membrane Transport, 4th ed.(Garland Science, 2002).

Li, K. C. P.

E. Sassaroli, K. C. P. Li, and B. E. O’Neill, Phys. Med. Biol. 54, 5541 (2009).
[CrossRef]

O’Neill, B. E.

E. Sassaroli, K. C. P. Li, and B. E. O’Neill, Phys. Med. Biol. 54, 5541 (2009).
[CrossRef]

Prausnitz, M. R.

P. Chakravarty, W. Qian, M. A. El-Sayed, and M. R. Prausnitz, Nat. Nanotechnol. 5, 607 (2010).
[CrossRef]

Qian, W.

P. Chakravarty, W. Qian, M. A. El-Sayed, and M. R. Prausnitz, Nat. Nanotechnol. 5, 607 (2010).
[CrossRef]

Qu, X.

C. Yao, X. Qu, Z. Zhang, G. Hüttmann, and R. Rahmanzadeh, J. Biomed. Opt. 14, 054034 (2009).
[CrossRef]

Raff, M.

B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Principles of Membrane Transport, 4th ed.(Garland Science, 2002).

Rahmanzadeh, R.

C. Yao, X. Qu, Z. Zhang, G. Hüttmann, and R. Rahmanzadeh, J. Biomed. Opt. 14, 054034 (2009).
[CrossRef]

Roberts, K.

B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Principles of Membrane Transport, 4th ed.(Garland Science, 2002).

Sassaroli, E.

E. Sassaroli, K. C. P. Li, and B. E. O’Neill, Phys. Med. Biol. 54, 5541 (2009).
[CrossRef]

Stevenson, D. J.

D. J. Stevenson, F. J. Gunn-Moore, P. Campbell, and K. Dholakia, J. R. Soc. Interface 7, 863 (2010).
[CrossRef]

Viator, J. A.

K. Bhattacharyya, B. S. Goldschmidt, M. Hannink, S. Alexander, A. Jurkevic, and J. A. Viator, Clin. Lab. Med. 32, 89 (2012).
[CrossRef]

Walter, P.

B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Principles of Membrane Transport, 4th ed.(Garland Science, 2002).

Yao, C.

C. Yao, X. Qu, Z. Zhang, G. Hüttmann, and R. Rahmanzadeh, J. Biomed. Opt. 14, 054034 (2009).
[CrossRef]

Zhang, Z.

C. Yao, X. Qu, Z. Zhang, G. Hüttmann, and R. Rahmanzadeh, J. Biomed. Opt. 14, 054034 (2009).
[CrossRef]

Acta Physiol. Scand. (1)

J. Gehl, Acta Physiol. Scand. 177, 437 (2003).
[CrossRef]

Clin. Lab. Med. (1)

K. Bhattacharyya, B. S. Goldschmidt, M. Hannink, S. Alexander, A. Jurkevic, and J. A. Viator, Clin. Lab. Med. 32, 89 (2012).
[CrossRef]

J. Biomed. Opt. (1)

C. Yao, X. Qu, Z. Zhang, G. Hüttmann, and R. Rahmanzadeh, J. Biomed. Opt. 14, 054034 (2009).
[CrossRef]

J. R. Soc. Interface (1)

D. J. Stevenson, F. J. Gunn-Moore, P. Campbell, and K. Dholakia, J. R. Soc. Interface 7, 863 (2010).
[CrossRef]

Nat. Nanotechnol. (1)

P. Chakravarty, W. Qian, M. A. El-Sayed, and M. R. Prausnitz, Nat. Nanotechnol. 5, 607 (2010).
[CrossRef]

Phys. Med. Biol. (1)

E. Sassaroli, K. C. P. Li, and B. E. O’Neill, Phys. Med. Biol. 54, 5541 (2009).
[CrossRef]

Other (1)

B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Principles of Membrane Transport, 4th ed.(Garland Science, 2002).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (2)

Fig. 1.
Fig. 1.

(a) Permeability results show a steady increase for all radiant exposures from 200 to 800 pulses except for 40mJ/cm2. (b) The nonviability plot shows a slow increase in cell death for 10 and 20mJ/cm2 but a much more rapid climb for 40mJ/cm2 for the same range of pulses.

Fig. 2.
Fig. 2.

Fluorescence image of increasing cell permeability from (a) 200, (b) 400, (c) 600, and (d) 800 pulses for 20mJ/cm2 in different irradiated regions of the cell culture dish. The blue regions indicate cells that have internalized Calcofluor White, while dark regions indicate cells that have not. Scale bar is 200 μm.

Metrics