Abstract

A simple and practical method for measuring the angle-resolved light scattering (ARLS) from individual objects is reported. Employing the principle of inline holography and a Fourier transform light scattering technique, both the static and dynamic scattering patterns from individual micrometer-sized objects can be effectively and quantitatively obtained. First, the light scattering measurements were performed on individual polystyrene beads, from which the refractive index and diameter of each bead were retrieved. Also, the measurements of the static and dynamic light scattering from intact human red blood cells are demonstrated. Using the present method, an existing microscope can be directly transformed into a precise instrument for ARLS measurements.

© 2012 Optical Society of America

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References

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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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2012 (2)

Y. Kim, J. M. Higgins, R. R. Dasari, S. Suresh, and Y. K. Park, J. Biomed. Opt. 17, 040501 (2012).
[CrossRef]

H. S. Yu, H. J. Park, Y. Kim, M. W. Kim, and Y. K. Park, Opt. Lett. 37, 2577 (2012).
[CrossRef]

2011 (1)

Y. Park, C. A. Best-Popescu, R. R. Dasari, and G. Popescu, J. Biomed. Opt. 16, 011013 (2011).
[CrossRef]

2010 (5)

Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, J. Biomed. Opt. 15, 020506 (2010).
[CrossRef]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

O. Mudanyali, C. Oztoprak, D. Tseng, A. Erlinger, and A. Ozcan, LAB On Chip 10, 2419 (2010).
[CrossRef]

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

H. Ding, L. J. Millet, M. U. Gillette, and G. Popescu, Biomed. Opt. Express 1, 260 (2010).
[CrossRef]

2009 (2)

2008 (1)

H. Ding, Z. Wang, F. Nguyen, S. A. Boppart, and G. Popescu, Phys. Rev. Lett. 101, 238102 (2008).
[CrossRef]

2007 (1)

2006 (2)

2001 (1)

W. Xu, M. Jericho, I. Meinertzhagen, and H. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

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1987 (1)

1979 (1)

D. Byrne and J. Earnshaw, J. Phys. D 12, 1133 (1979).
[CrossRef]

Badizadegan, K.

Barman, I.

Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, J. Biomed. Opt. 15, 020506 (2010).
[CrossRef]

Berne, B.

B. Berne and R. Pecora, Dynamic Light Scattering: With Applications to Chemistry, Biology, and Physics (Dover, 2000).

Best-Popescu, C. A.

Y. Park, C. A. Best-Popescu, R. R. Dasari, and G. Popescu, J. Biomed. Opt. 16, 011013 (2011).
[CrossRef]

Bishara, W.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

Blaaderen, A.

Boppart, M. D.

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

Boppart, S. A.

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

H. Ding, F. Nguyen, S. A. Boppart, and G. Popescu, Opt. Lett. 34, 1372 (2009).
[CrossRef]

H. Ding, Z. Wang, F. Nguyen, S. A. Boppart, and G. Popescu, Phys. Rev. Lett. 101, 238102 (2008).
[CrossRef]

Byrne, D.

D. Byrne and J. Earnshaw, J. Phys. D 12, 1133 (1979).
[CrossRef]

Choi, W.

Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, J. Biomed. Opt. 15, 020506 (2010).
[CrossRef]

D. Fu, W. Choi, Y. Sung, S. Oh, Z. Yaqoob, Y. Park, R. R. Dasari, and M. S. Feld, Opt. Express 17, 18878 (2009).
[CrossRef]

Dasari, R. R.

David, D. G.

Diez-Silva, M.

Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, J. Biomed. Opt. 15, 020506 (2010).
[CrossRef]

Ding, H.

H. Ding, L. J. Millet, M. U. Gillette, and G. Popescu, Biomed. Opt. Express 1, 260 (2010).
[CrossRef]

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

H. Ding, F. Nguyen, S. A. Boppart, and G. Popescu, Opt. Lett. 34, 1372 (2009).
[CrossRef]

H. Ding, Z. Wang, F. Nguyen, S. A. Boppart, and G. Popescu, Phys. Rev. Lett. 101, 238102 (2008).
[CrossRef]

Earnshaw, J.

D. Byrne and J. Earnshaw, J. Phys. D 12, 1133 (1979).
[CrossRef]

Erlinger, A.

O. Mudanyali, C. Oztoprak, D. Tseng, A. Erlinger, and A. Ozcan, LAB On Chip 10, 2419 (2010).
[CrossRef]

Feld, M. S.

Fu, D.

Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, J. Biomed. Opt. 15, 020506 (2010).
[CrossRef]

D. Fu, W. Choi, Y. Sung, S. Oh, Z. Yaqoob, Y. Park, R. R. Dasari, and M. S. Feld, Opt. Express 17, 18878 (2009).
[CrossRef]

Gillette, M. U.

H. Ding, L. J. Millet, M. U. Gillette, and G. Popescu, Biomed. Opt. Express 1, 260 (2010).
[CrossRef]

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

Higgins, J. M.

Y. Kim, J. M. Higgins, R. R. Dasari, S. Suresh, and Y. K. Park, J. Biomed. Opt. 17, 040501 (2012).
[CrossRef]

Ikeda, T.

Isikman, S. O.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

Jericho, M.

W. Xu, M. Jericho, I. Meinertzhagen, and H. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Joyeux, D.

Khademhosseini, B.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

Kim, M. W.

Kim, S.-H.

Kim, Y.

Y. Kim, J. M. Higgins, R. R. Dasari, S. Suresh, and Y. K. Park, J. Biomed. Opt. 17, 040501 (2012).
[CrossRef]

H. S. Yu, H. J. Park, Y. Kim, M. W. Kim, and Y. K. Park, Opt. Lett. 37, 2577 (2012).
[CrossRef]

Koren, G.

Kreuzer, H.

W. Xu, M. Jericho, I. Meinertzhagen, and H. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Lee, S.-H.

Liu, G.

Liu, J.

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

Meinertzhagen, I.

W. Xu, M. Jericho, I. Meinertzhagen, and H. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Millet, L. J.

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

H. Ding, L. J. Millet, M. U. Gillette, and G. Popescu, Biomed. Opt. Express 1, 260 (2010).
[CrossRef]

Mudanyali, O.

O. Mudanyali, C. Oztoprak, D. Tseng, A. Erlinger, and A. Ozcan, LAB On Chip 10, 2419 (2010).
[CrossRef]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

Nguyen, F.

H. Ding, F. Nguyen, S. A. Boppart, and G. Popescu, Opt. Lett. 34, 1372 (2009).
[CrossRef]

H. Ding, Z. Wang, F. Nguyen, S. A. Boppart, and G. Popescu, Phys. Rev. Lett. 101, 238102 (2008).
[CrossRef]

Nguyen, F. T.

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

Oh, C.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

Oh, S.

Oostrum, P.

Ozcan, A.

O. Mudanyali, C. Oztoprak, D. Tseng, A. Erlinger, and A. Ozcan, LAB On Chip 10, 2419 (2010).
[CrossRef]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

Oztoprak, C.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

O. Mudanyali, C. Oztoprak, D. Tseng, A. Erlinger, and A. Ozcan, LAB On Chip 10, 2419 (2010).
[CrossRef]

Park, H. J.

Park, Y.

Y. Park, C. A. Best-Popescu, R. R. Dasari, and G. Popescu, J. Biomed. Opt. 16, 011013 (2011).
[CrossRef]

Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, J. Biomed. Opt. 15, 020506 (2010).
[CrossRef]

D. Fu, W. Choi, Y. Sung, S. Oh, Z. Yaqoob, Y. Park, R. R. Dasari, and M. S. Feld, Opt. Express 17, 18878 (2009).
[CrossRef]

Park, Y. K.

Pecora, R.

B. Berne and R. Pecora, Dynamic Light Scattering: With Applications to Chemistry, Biology, and Physics (Dover, 2000).

Polack, F.

Popescu, G.

Y. Park, C. A. Best-Popescu, R. R. Dasari, and G. Popescu, J. Biomed. Opt. 16, 011013 (2011).
[CrossRef]

H. Ding, L. J. Millet, M. U. Gillette, and G. Popescu, Biomed. Opt. Express 1, 260 (2010).
[CrossRef]

Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, J. Biomed. Opt. 15, 020506 (2010).
[CrossRef]

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

H. Ding, F. Nguyen, S. A. Boppart, and G. Popescu, Opt. Lett. 34, 1372 (2009).
[CrossRef]

H. Ding, Z. Wang, F. Nguyen, S. A. Boppart, and G. Popescu, Phys. Rev. Lett. 101, 238102 (2008).
[CrossRef]

Y. K. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Express 14, 8263 (2006).
[CrossRef]

G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, Opt. Lett. 31, 775 (2006).
[CrossRef]

Roichman, Y.

Scott, P. D.

Sencan, I.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

Seo, S. K.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

Sung, Y.

Suresh, S.

Y. Kim, J. M. Higgins, R. R. Dasari, S. Suresh, and Y. K. Park, J. Biomed. Opt. 17, 040501 (2012).
[CrossRef]

Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, J. Biomed. Opt. 15, 020506 (2010).
[CrossRef]

Tseng, D.

O. Mudanyali, C. Oztoprak, D. Tseng, A. Erlinger, and A. Ozcan, LAB On Chip 10, 2419 (2010).
[CrossRef]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

Wang, Z.

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

H. Ding, Z. Wang, F. Nguyen, S. A. Boppart, and G. Popescu, Phys. Rev. Lett. 101, 238102 (2008).
[CrossRef]

Xu, W.

W. Xu, M. Jericho, I. Meinertzhagen, and H. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Yang, S.-M.

Yaqoob, Z.

Yu, H. S.

Biomed. Opt. Express (1)

J. Biomed. Opt. (3)

Y. Park, C. A. Best-Popescu, R. R. Dasari, and G. Popescu, J. Biomed. Opt. 16, 011013 (2011).
[CrossRef]

Y. Park, M. Diez-Silva, D. Fu, G. Popescu, W. Choi, I. Barman, S. Suresh, and M. S. Feld, J. Biomed. Opt. 15, 020506 (2010).
[CrossRef]

Y. Kim, J. M. Higgins, R. R. Dasari, S. Suresh, and Y. K. Park, J. Biomed. Opt. 17, 040501 (2012).
[CrossRef]

J. Comput. Theor. Nanosci. (1)

H. Ding, Z. Wang, F. T. Nguyen, S. A. Boppart, L. J. Millet, M. U. Gillette, J. Liu, M. D. Boppart, and G. Popescu, J. Comput. Theor. Nanosci. 7, 2501 (2010).
[CrossRef]

J. Opt. Soc. Am. A (2)

J. Phys. D (1)

D. Byrne and J. Earnshaw, J. Phys. D 12, 1133 (1979).
[CrossRef]

LAB On Chip (2)

O. Mudanyali, C. Oztoprak, D. Tseng, A. Erlinger, and A. Ozcan, LAB On Chip 10, 2419 (2010).
[CrossRef]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. K. Seo, B. Khademhosseini, and A. Ozcan, LAB On Chip 10, 1417 (2010).
[CrossRef]

Opt. Express (3)

Opt. Lett. (3)

Phys. Rev. Lett. (1)

H. Ding, Z. Wang, F. Nguyen, S. A. Boppart, and G. Popescu, Phys. Rev. Lett. 101, 238102 (2008).
[CrossRef]

Proc. Natl. Acad. Sci. USA (1)

W. Xu, M. Jericho, I. Meinertzhagen, and H. Kreuzer, Proc. Natl. Acad. Sci. USA 98, 11301 (2001).
[CrossRef]

Other (1)

B. Berne and R. Pecora, Dynamic Light Scattering: With Applications to Chemistry, Biology, and Physics (Dover, 2000).

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Figures (3)

Fig. 1.
Fig. 1.

Experimental setup and principle of the FTLS using inline holography.

Fig. 2.
Fig. 2.

Light scattering of polystyrene beads. (a) Original inline hologram. (b) and (c) Retrieved amplitude and wrapped phase images of the scalar E field. (d) Scattering intensity pattern of the bead. (e) Light scattering pattern as a function of the scattering angle from 0 to 20° after applying azimuthal average. The area represents standard deviation for different azimutal angles. (f) Distribution of the retrieved refractive indexes and diameters.

Fig. 3.
Fig. 3.

Static and DLS of individual human RBCs. (a) Measured inline hologram of an RBC. (b) Phase image of the RBC in (a). (c) Light scattering pattern of the RBC, (inset) the DLS autocorrelation G(τ) at θ=8°. (d) Retrieved ω0 and Γ of 46 RBCs from the temporal correlation fluctuation of the DLS signal.

Equations (2)

Equations on this page are rendered with MathJax. Learn more.

I(qx,qy)=|12πU(x,y)ejqxxejqxxdxdy|2,
G(τ)=A+Bcos(ω0τ+φ)exp(Γτ)exp(β2τ2),

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