We propose a label-free depth-resolved tomographic scheme for imaging a single live cell in fluid. This approach utilizes a modified time-domain full-field optical coherence tomography (FF-OCT) system combined with an optical tweezer technique. The optical trap for holding a moving specimen is made by tightly focusing a 1064 nm -switching pulsed laser beam with a 1.0 NA microscope objective in the sample arm of the FF-OCT part. By cosharing the probe for both systems, the optical actions of trapping and cellular resolution tomographic imaging could be achieved simultaneously. Feasibility of the combined system is demonstrated by imaging micron-sized polystyrene beads and a living suspension cell in medium.
©2012 Optical Society of AmericaFull Article | PDF Article
OSA Recommended Articles
Avner Safrani and Ibrahim Abdulhalim
Opt. Lett. 37(4) 458-460 (2012)
Opt. Express 20(9) 9962-9977 (2012)
Jiaying Xiao, Bo Wang, Guangyin Lu, Ziqiang Zhu, and Yujiao Huang
Appl. Opt. 51(16) 3650-3654 (2012)