Abstract

We propose a label-free depth-resolved tomographic scheme for imaging a single live cell in fluid. This approach utilizes a modified time-domain full-field optical coherence tomography (FF-OCT) system combined with an optical tweezer technique. The optical trap for holding a moving specimen is made by tightly focusing a 1064 nm Q-switching pulsed laser beam with a 1.0 NA microscope objective in the sample arm of the FF-OCT part. By cosharing the probe for both systems, the optical actions of trapping and cellular resolution tomographic imaging could be achieved simultaneously. Feasibility of the combined system is demonstrated by imaging micron-sized polystyrene beads and a living suspension cell in medium.

© 2012 Optical Society of America

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2010 (5)

2007 (1)

A. M. Zysk, F. T. Nguyen, A. L. Oldenburg, D. L. Marks, and S. A. Boppart, J. Biomed. Opt. 12, 051403 (2007).
[CrossRef]

2006 (2)

2004 (2)

1996 (1)

S. B. Smith, Y. Cui, and C. Bustamante, Science 271, 795 (1996).
[CrossRef]

1991 (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

1987 (1)

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef]

1986 (1)

Agate, B.

Ahn, S.-G.

Ashkin, A.

Bjorkholm, J. E.

Boccara, C.

Boppart, S. A.

A. M. Zysk, F. T. Nguyen, A. L. Oldenburg, D. L. Marks, and S. A. Boppart, J. Biomed. Opt. 12, 051403 (2007).
[CrossRef]

W. Tan, A. L. Oldenburg, J. J. Norman, T. A. Desai, and S. A. Boppart, Opt. Express 14, 7159 (2006).
[CrossRef]

Brown, C. T. A.

Bustamante, C.

S. B. Smith, Y. Cui, and C. Bustamante, Science 271, 795 (1996).
[CrossRef]

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Choi, W. J.

Chu, S.

Cui, Y.

S. B. Smith, Y. Cui, and C. Bustamante, Science 271, 795 (1996).
[CrossRef]

Desai, T. A.

Dhalla, A.-H.

Dholakia, K.

Dubois, A.

Dziedzic, J. M.

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Fujimoto, J. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Grieve, K.

Han, J.-H.

Hee, M. R.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Izatt, J. A.

Jeon, D. I.

Kang, J. U.

Kim, S.

Lasser, T.

C. Pache, M. L. Villiger, and T. Lasser, Proc. SPIE 7554, 755425 (2010).
[CrossRef]

Lecaque, R.

Lee, B. H.

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Liu, X.

Marks, D. L.

A. M. Zysk, F. T. Nguyen, A. L. Oldenburg, D. L. Marks, and S. A. Boppart, J. Biomed. Opt. 12, 051403 (2007).
[CrossRef]

Migacz, J. V.

Moneron, G.

Nguyen, F. T.

A. M. Zysk, F. T. Nguyen, A. L. Oldenburg, D. L. Marks, and S. A. Boppart, J. Biomed. Opt. 12, 051403 (2007).
[CrossRef]

Norman, J. J.

Oldenburg, A. L.

A. M. Zysk, F. T. Nguyen, A. L. Oldenburg, D. L. Marks, and S. A. Boppart, J. Biomed. Opt. 12, 051403 (2007).
[CrossRef]

W. Tan, A. L. Oldenburg, J. J. Norman, T. A. Desai, and S. A. Boppart, Opt. Express 14, 7159 (2006).
[CrossRef]

Pache, C.

C. Pache, M. L. Villiger, and T. Lasser, Proc. SPIE 7554, 755425 (2010).
[CrossRef]

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Reihani, N. S.

Samadi, A.

Sarunic, M. V.

Schuman, J. S.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Sibbett, W.

Smith, S. B.

S. B. Smith, Y. Cui, and C. Bustamante, Science 271, 795 (1996).
[CrossRef]

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Swanson, E. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

Tan, W.

Vabre, L.

Villiger, M. L.

C. Pache, M. L. Villiger, and T. Lasser, Proc. SPIE 7554, 755425 (2010).
[CrossRef]

Weinberg, S.

Yamane, T.

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef]

Yoon, J.-H.

Zhang, K.

Zysk, A. M.

A. M. Zysk, F. T. Nguyen, A. L. Oldenburg, D. L. Marks, and S. A. Boppart, J. Biomed. Opt. 12, 051403 (2007).
[CrossRef]

Appl. Opt. (1)

J. Biomed. Opt. (1)

A. M. Zysk, F. T. Nguyen, A. L. Oldenburg, D. L. Marks, and S. A. Boppart, J. Biomed. Opt. 12, 051403 (2007).
[CrossRef]

J. Opt. Soc. Korea (1)

Nature (1)

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef]

Opt. Express (3)

Opt. Lett. (4)

Proc. SPIE (1)

C. Pache, M. L. Villiger, and T. Lasser, Proc. SPIE 7554, 755425 (2010).
[CrossRef]

Science (2)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef]

S. B. Smith, Y. Cui, and C. Bustamante, Science 271, 795 (1996).
[CrossRef]

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Figures (5)

Fig. 1.
Fig. 1.

Schematic of the FF-OCT/OT system. The lower left graph shows a spectrum of femo-second laser as an FF-OCT light source. LP, linear polarizer; MO, microscope objective (water-immersion, 100×, 1.0 NA in water); PZT, piezo-electric transducer.

Fig. 2.
Fig. 2.

Representative FF-OCT images of 5 μm polystyrene beads in a water chamber: A, trapped bead; B, control bead (one of the untrapped beads).

Fig. 3.
Fig. 3.

(a) Bright-field image of 11 μm polystyrene beads in a water chamber; (b) its correspoding FF-OCT image; (c)–(g) representative FF-OCT images of the beads with the axial movement of the water chamber.While tweezing only the single bead (A), the water chamber is axially (Z) moved down with a step of 0.5 μm. Insets of each figure are enlarged views of the trapped bead A and the control bead B.

Fig. 4.
Fig. 4.

Still images demonstraing optical trapping of a single cell against the movement of the culture plate.

Fig. 5.
Fig. 5.

(a) Microscopic image of three individual THP-1 cells in culture medium (cell 2, trapped; cell 1,3, untrapped); (b) FF-OCT image of the trapped cell; (c)–(e) depth-resolved FF-OCT images of the THP-1 cell taken with a step of 2.5 μm.

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