Abstract

The intrinsic aberrations of high-NA gradient refractive index (GRIN) lenses limit their image quality as well as field of view. Here we used a pupil-segmentation-based adaptive optical approach to correct the inherent aberrations in a two-photon fluorescence endoscope utilizing a 0.8 NA GRIN lens. By correcting the field-dependent aberrations, we recovered diffraction-limited performance across a large imaging field. The consequent improvements in imaging signal and resolution allowed us to detect fine structures that were otherwise invisible inside mouse brain slices.

© 2012 Optical Society of America

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    [CrossRef]
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    [CrossRef]
  9. GRINTECH. Datasheet “High NA for 2-Photon Microscopy,” available from http://www.grintech.de/grin-lens-systems-for-medical-applications.html .

2012

N. Ji, T. R. Sato, and E. Betzig, Proc. Nat. Acad. Sci. USA 109, 22 (2012).
[CrossRef]

2011

F. Bortoletto, C. Bonoli, P. Panizzolo, C. D. Ciubotaru, and F. Mammano, PLoS ONE 6, e22321 (2011).
[CrossRef]

W. M. Lee and S. H. Yun, Opt. Lett. 36, 4608 (2011).
[CrossRef]

2010

N. Ji, D. E. Milkie, and E. Betzig, Nat. Meth. 7, 141 (2010).
[CrossRef]

2009

R. P. Barretto, B. Messerschmidt, and M. J. Schnitzer, Nat. Meth. 6, 511 (2009).
[CrossRef]

2003

1980

Bao, C.

C. Gomez-Reino, M. V. Perez, and C. Bao, Gradient-Index Optics (Springer-Verlag, 2002).

Barretto, R. P.

R. P. Barretto, B. Messerschmidt, and M. J. Schnitzer, Nat. Meth. 6, 511 (2009).
[CrossRef]

Betzig, E.

N. Ji, T. R. Sato, and E. Betzig, Proc. Nat. Acad. Sci. USA 109, 22 (2012).
[CrossRef]

N. Ji, D. E. Milkie, and E. Betzig, Nat. Meth. 7, 141 (2010).
[CrossRef]

Bonoli, C.

F. Bortoletto, C. Bonoli, P. Panizzolo, C. D. Ciubotaru, and F. Mammano, PLoS ONE 6, e22321 (2011).
[CrossRef]

Bortoletto, F.

F. Bortoletto, C. Bonoli, P. Panizzolo, C. D. Ciubotaru, and F. Mammano, PLoS ONE 6, e22321 (2011).
[CrossRef]

Ciubotaru, C. D.

F. Bortoletto, C. Bonoli, P. Panizzolo, C. D. Ciubotaru, and F. Mammano, PLoS ONE 6, e22321 (2011).
[CrossRef]

Gomez-Reino, C.

C. Gomez-Reino, M. V. Perez, and C. Bao, Gradient-Index Optics (Springer-Verlag, 2002).

Ji, N.

N. Ji, T. R. Sato, and E. Betzig, Proc. Nat. Acad. Sci. USA 109, 22 (2012).
[CrossRef]

N. Ji, D. E. Milkie, and E. Betzig, Nat. Meth. 7, 141 (2010).
[CrossRef]

Jung, J. C.

Lee, W. M.

Mammano, F.

F. Bortoletto, C. Bonoli, P. Panizzolo, C. D. Ciubotaru, and F. Mammano, PLoS ONE 6, e22321 (2011).
[CrossRef]

Messerschmidt, B.

R. P. Barretto, B. Messerschmidt, and M. J. Schnitzer, Nat. Meth. 6, 511 (2009).
[CrossRef]

Milkie, D. E.

N. Ji, D. E. Milkie, and E. Betzig, Nat. Meth. 7, 141 (2010).
[CrossRef]

Panizzolo, P.

F. Bortoletto, C. Bonoli, P. Panizzolo, C. D. Ciubotaru, and F. Mammano, PLoS ONE 6, e22321 (2011).
[CrossRef]

Perez, M. V.

C. Gomez-Reino, M. V. Perez, and C. Bao, Gradient-Index Optics (Springer-Verlag, 2002).

Sato, T. R.

N. Ji, T. R. Sato, and E. Betzig, Proc. Nat. Acad. Sci. USA 109, 22 (2012).
[CrossRef]

Schnitzer, M. J.

R. P. Barretto, B. Messerschmidt, and M. J. Schnitzer, Nat. Meth. 6, 511 (2009).
[CrossRef]

J. C. Jung and M. J. Schnitzer, Opt. Lett. 28, 902 (2003).
[CrossRef]

Tomlinson, W. J.

Yun, S. H.

Appl. Opt.

Nat. Meth.

R. P. Barretto, B. Messerschmidt, and M. J. Schnitzer, Nat. Meth. 6, 511 (2009).
[CrossRef]

N. Ji, D. E. Milkie, and E. Betzig, Nat. Meth. 7, 141 (2010).
[CrossRef]

Opt. Lett.

PLoS ONE

F. Bortoletto, C. Bonoli, P. Panizzolo, C. D. Ciubotaru, and F. Mammano, PLoS ONE 6, e22321 (2011).
[CrossRef]

Proc. Nat. Acad. Sci. USA

N. Ji, T. R. Sato, and E. Betzig, Proc. Nat. Acad. Sci. USA 109, 22 (2012).
[CrossRef]

Other

C. Gomez-Reino, M. V. Perez, and C. Bao, Gradient-Index Optics (Springer-Verlag, 2002).

GRINTECH. Datasheet “High NA for 2-Photon Microscopy,” available from http://www.grintech.de/grin-lens-systems-for-medical-applications.html .

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