Abstract

Although ultrasound arrays have been exploited in photoacoustic imaging to improve imaging speed, ultrasound-array-based optical-resolution photoacoustic microscopy (OR-PAM) has never been achieved previously to our knowledge. Here we present our development of multifocal OR-PAM using a microlens array for optical illumination and an ultrasound array for photoacoustic detection. Our system is capable of imaging hemoglobin concentration and oxygenation in individual microvessels in vivo at high speed. Compared with a single focus, multiple foci reduce the scanning load and increase the imaging speed significantly. The current multifocal system can acquire 1000×500×200 voxels at ∼10 μm lateral resolution within 4 min.

© 2011 Optical Society of America

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  1. M. D. Stern, Nature 254, 56 (1975).
    [CrossRef]
  2. E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
    [CrossRef]
  3. E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
    [CrossRef]
  4. R. K. Wang and L. An, Opt. Express 17, 8926 (2009).
    [CrossRef]
  5. K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008).
    [CrossRef]
  6. S. Hu and L. V. Wang, J Biomed. Opt. 15, 011101 (2010).
    [CrossRef]
  7. Z. X. Xie, S. L. Jiao, H. F. Zhang, and C. A. Puliafito, Opt. Lett. 34, 1771 (2009).
    [CrossRef]
  8. B. Rao, L. Li, K. Maslov, and L. V. Wang, Opt. Lett. 35, 1521 (2010).
    [CrossRef]
  9. J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
    [CrossRef]
  10. R. J. Zemp, L. Song, R. Bitton, K. K. Shung, and L. V. Wang, Opt. Express 16, 7915 (2008).
    [CrossRef]
  11. L. Song, K. Maslov, and L. V. Wang, Opt. Lett. 35, 1482 (2010).
    [CrossRef]
  12. L. Song, K. Maslov, R. Bitton, K. K. Shung, and L. V. Wang, J Biomed. Opt. 13, 054028 (2008).
    [CrossRef]
  13. S. Hu, K. Maslov, and L. V. Wang, Opt. Express 17, 7688 (2009).
    [CrossRef]
  14. American National Standard for Safe Use of Lasers, Tech. Rep. “Standard for the safe use of lasers,” ANSI Z136.1-2000 (American National Standards Institute, 2000).

2010

2009

2008

2005

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef]

2004

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef]

2003

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef]

1975

M. D. Stern, Nature 254, 56 (1975).
[CrossRef]

An, L.

Audinat, E.

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef]

Bitton, R.

L. Song, K. Maslov, R. Bitton, K. K. Shung, and L. V. Wang, J Biomed. Opt. 13, 054028 (2008).
[CrossRef]

R. J. Zemp, L. Song, R. Bitton, K. K. Shung, and L. V. Wang, Opt. Express 16, 7915 (2008).
[CrossRef]

Chaigneau, E.

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef]

Charpak, S.

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef]

Frenz, M.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef]

Genet, M.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef]

Hu, S.

Jaeger, M.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef]

Jiao, S. L.

Laemmel, E.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef]

Le Gargasson, J. F.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef]

Le Goualher, G.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef]

Lemor, R.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef]

Li, L.

Maslov, K.

Niederhauser, J. J.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef]

Oheim, M.

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef]

Perchant, A.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef]

Puliafito, C. A.

Rao, B.

Shung, K. K.

R. J. Zemp, L. Song, R. Bitton, K. K. Shung, and L. V. Wang, Opt. Express 16, 7915 (2008).
[CrossRef]

L. Song, K. Maslov, R. Bitton, K. K. Shung, and L. V. Wang, J Biomed. Opt. 13, 054028 (2008).
[CrossRef]

Song, L.

Stern, M. D.

M. D. Stern, Nature 254, 56 (1975).
[CrossRef]

Vicaut, E.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef]

Wang, L. V.

Wang, R. K.

Weber, P.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef]

Xie, Z. X.

Zemp, R. J.

Zhang, H. F.

IEEE Trans. Med. Imaging

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef]

J Biomed. Opt.

S. Hu and L. V. Wang, J Biomed. Opt. 15, 011101 (2010).
[CrossRef]

L. Song, K. Maslov, R. Bitton, K. K. Shung, and L. V. Wang, J Biomed. Opt. 13, 054028 (2008).
[CrossRef]

J. Vasc. Res.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef]

Nature

M. D. Stern, Nature 254, 56 (1975).
[CrossRef]

Opt. Express

Opt. Lett.

Proc. Natl. Acad. Sci. USA

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef]

Other

American National Standard for Safe Use of Lasers, Tech. Rep. “Standard for the safe use of lasers,” ANSI Z136.1-2000 (American National Standards Institute, 2000).

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