Abstract

We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO2) and oxygen partial pressure (pO2) in vivo in single blood vessels with high spatial resolution. While PAM measures sO2 by imaging hemoglobin optical absorption at two wavelengths, FCM quantifies pO2 using phosphorescence quenching. The variations of sO2 and pO2 values in multiple orders of vessel branches under hyperoxic (100% oxygen) and normoxic (21% oxygen) conditions correlate well with the oxygen–hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength.

© 2011 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Microvascular oxygen quantification using two-photon microscopy

Arnold D. Estrada, Adrien Ponticorvo, Tim N. Ford, and Andrew K. Dunn
Opt. Lett. 33(10) 1038-1040 (2008)

Snap-shot multispectral imaging of vascular dynamics in a mouse window-chamber model

Hansford C. Hendargo, Yulin Zhao, Taylor Allenby, and Gregory M. Palmer
Opt. Lett. 40(14) 3292-3295 (2015)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription