Abstract

We report a technique for measuring and correcting the wavefront aberrations introduced by a biological sample using a Shack–Hartmann wavefront sensor, a fluorescent reference source, and a deformable mirror. The reference source and sample fluorescence are at different wavelengths to separate wavefront measurement and sample imaging. The measurement and correction at one wavelength improves the resolving power at a different wavelength, enabling the structure of the sample to be resolved.

© 2011 Optical Society of America

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    [CrossRef]
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2010 (2)

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010).
[CrossRef] [PubMed]

2009 (1)

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

2007 (3)

M. J. Booth, Phil. Trans. R. Soc. A 365, 2829 (2007).
[CrossRef] [PubMed]

M. Schwertner, M. J. Booth, and T. Wilson, J. Microsc. 228, 97 (2007).
[CrossRef] [PubMed]

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

2006 (1)

K. Svoboda and R. Yasuda, Neuron 50, 823 (2006).
[CrossRef] [PubMed]

2004 (1)

2003 (1)

D. T. Gavel, Proc. SPIE 4839, 972 (2003).
[CrossRef]

1999 (1)

1996 (1)

A. Dunn and R. Richards-Kortum, IEEE J. Sel. Top. Quantum Electron. 2, 898 (1996).
[CrossRef]

1994 (1)

Azucena, O.

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010).
[CrossRef] [PubMed]

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

Bille, J. F.

Booth, M. J.

M. J. Booth, Phil. Trans. R. Soc. A 365, 2829 (2007).
[CrossRef] [PubMed]

M. Schwertner, M. J. Booth, and T. Wilson, J. Microsc. 228, 97 (2007).
[CrossRef] [PubMed]

Cao, J.

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010).
[CrossRef] [PubMed]

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

Condeelis, J.

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

Crest, J.

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010).
[CrossRef] [PubMed]

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

Dainty, J. C.

Denk, W.

Dillon, D.

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010).
[CrossRef] [PubMed]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

Dunn, A.

A. Dunn and R. Richards-Kortum, IEEE J. Sel. Top. Quantum Electron. 2, 898 (1996).
[CrossRef]

Feierabend, M.

Gavel, D.

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010).
[CrossRef] [PubMed]

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

Gavel, D. T.

D. T. Gavel, Proc. SPIE 4839, 972 (2003).
[CrossRef]

Goelz, S.

Goswami, S.

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

Grim, B.

Hardy, J. W.

J. W. Hardy, Adaptive Optics for Astronomical Telescopes (Oxford University Press, 1998).

Haro, L. D. S.

Kner, P.

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010).
[CrossRef] [PubMed]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

Kubby, J.

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010).
[CrossRef] [PubMed]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

Li, J. F.

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

Liang, J.

Lin, E. Y.

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

Olivier, S.

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010).
[CrossRef] [PubMed]

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

Pollard, J. W.

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

Richards-Kortum, R.

A. Dunn and R. Richards-Kortum, IEEE J. Sel. Top. Quantum Electron. 2, 898 (1996).
[CrossRef]

Ruckel, M.

Schwertner, M.

M. Schwertner, M. J. Booth, and T. Wilson, J. Microsc. 228, 97 (2007).
[CrossRef] [PubMed]

Segall, J. E.

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

Stanley, E. R.

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

Sullivan, W.

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010).
[CrossRef] [PubMed]

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

Svoboda, K.

K. Svoboda and R. Yasuda, Neuron 50, 823 (2006).
[CrossRef] [PubMed]

Thorn, K.

J.Porter, H.Queener, J.Lin, K. Thorn, and A.Awwal, eds., Adaptive Optics for Vision Science (Wiley-Interscience, 2006).
[CrossRef]

Wang, Y.

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

Wilson, T.

M. Schwertner, M. J. Booth, and T. Wilson, J. Microsc. 228, 97 (2007).
[CrossRef] [PubMed]

T. Wilson, in Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances, A.Diaspro, ed. (Wiley-Liss, 2002), p. 19.

Wyckoff, J. B.

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

Yasuda, R.

K. Svoboda and R. Yasuda, Neuron 50, 823 (2006).
[CrossRef] [PubMed]

Cancer Res. (1)

J. B. Wyckoff, Y. Wang, E. Y. Lin, J. F. Li, S. Goswami, E. R. Stanley, J. E. Segall, J. W. Pollard, and J. Condeelis, Cancer Res. 67, 2649 (2007).
[CrossRef] [PubMed]

IEEE J. Sel. Top. Quantum Electron. (1)

A. Dunn and R. Richards-Kortum, IEEE J. Sel. Top. Quantum Electron. 2, 898 (1996).
[CrossRef]

J. Microsc. (1)

M. Schwertner, M. J. Booth, and T. Wilson, J. Microsc. 228, 97 (2007).
[CrossRef] [PubMed]

J. Opt. Soc. Am. A (1)

Neuron (1)

K. Svoboda and R. Yasuda, Neuron 50, 823 (2006).
[CrossRef] [PubMed]

Opt. Express (1)

Opt. Lett. (2)

Phil. Trans. R. Soc. A (1)

M. J. Booth, Phil. Trans. R. Soc. A 365, 2829 (2007).
[CrossRef] [PubMed]

Proc. SPIE (3)

O. Azucena, J. Kubby, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, and S. Olivier, Proc. SPIE 7209, 720906(2009).
[CrossRef]

O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Proc. SPIE 7595, 75950I (2010).
[CrossRef]

D. T. Gavel, Proc. SPIE 4839, 972 (2003).
[CrossRef]

Other (3)

J. W. Hardy, Adaptive Optics for Astronomical Telescopes (Oxford University Press, 1998).

J.Porter, H.Queener, J.Lin, K. Thorn, and A.Awwal, eds., Adaptive Optics for Vision Science (Wiley-Interscience, 2006).
[CrossRef]

T. Wilson, in Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances, A.Diaspro, ed. (Wiley-Liss, 2002), p. 19.

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Figures (2)

Fig. 1
Fig. 1

Adaptive optics wide-field microscope setup. L1, L2, L3, L4 are 180, 85, 275, and 225 mm focal length lenses, respectively. Fold mirror D helps to bring the optical path into alignment for the SHWS.

Fig. 2
Fig. 2

AO off and on. (a) Green science camera image with AO system off and (b) with AO on. (c) Profile display of red line in Fig. 2a. (d) Profile display of red line in Fig. 2b. (a) and (b) have been normalized to their own minimum and maximum to display the clear boundaries between the objects. The white lines in (a) and (b) are approximately 1 μm in length.

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