Abstract

Flow cytometers are widely applied to environmental monitoring, industrial testing, and biochemical studies. Integrating a flow cytometer into microfluidic networks helps to miniaturize the system and make it portable for field use. The integration of optical components, such as lenses, further improves the compactness and thus has been intensively studied recently. However, the current designs suffer from severe light scattering due to the roughness of the solid-based lens interface. In this Letter, we propose a flow cytometer using an optofluidic lens to focus the light beam. Benefiting from the smooth liquid–liquid lens interface and the refractive-index matching liquid as cladding streams, a light beam can be well focused without scattering. The variations of the signal peak values are reduced, owing to the small beam width at the beam waist. The device presents an efficient and accurate performance on both the counting and sizing of particles.

© 2011 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Biconcave micro-optofluidic lens with low-refractive-index liquids

Chaolong Song, Nam-Trung Nguyen, Anand Krishna Asundi, and Cassandra Lee-Ngo Low
Opt. Lett. 34(23) 3622-3624 (2009)

Micro flow cytometer with self-aligned 3D hydrodynamic focusing

Genni Testa, Gianluca Persichetti, and Romeo Bernini
Biomed. Opt. Express 6(1) 54-62 (2015)

Formation and characterization of an ideal excitation beam geometry in an optofluidic device

Benjamin R. Watts, Thomas Kowpak, Zhiyi Zhang, Chang-Qing Xu, and Shiping Zhu
Biomed. Opt. Express 1(3) 848-860 (2010)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription