Abstract

In this Letter, the capability of label-free fiber-based optical-resolution photoacoustic microscopy is demonstrated. This real-time imaging system takes advantage of image-guide fibers and a unique fiber laser. The 800μm image-guide consists of 30,000 individual single-mode fibers in a bundle and the diode-pumped, pulsed Ytterbium fiber laser is utilized to perform up to 600kHz repetition rate. Phantom studies indicate 7μm resolution. The proposed setup keeps many of the powerful properties of previous tabletop OR-PAM systems, but also offers a submillimeter probe footprint and high flexibility due to the nature of the image-guide. This system could have significant clinical impact for endoscopic applications where the thin fiber can be inserted into the body.

© 2011 Optical Society of America

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References

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2011 (4)

W. Shi, P. Hajireza, P. Shao, A. Forbrich, and R. J. Zemp, Opt. Express 19, 17143 (2011).
[CrossRef] [PubMed]

X. Xu, H. Liu, and L. V. Wang, Nat. Photon. 5, 154 (2011).
[CrossRef]

P. Hajireza, W. Shi, P. Shao, S. Kerr, and R. J. Zemp, Proc. SPIE 7899, 78990 (2011).
[CrossRef]

S. P. Rajesh, D. Lorenser, and D. D. Sampson, Opt. Express 19, 7213 (2011).
[CrossRef]

2010 (1)

2009 (4)

2006 (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

2005 (1)

R. Manniesing and W. Niessen, Inf. Process Med. Imaging 19, 138 (2005).
[CrossRef]

2004 (1)

J. C. Jung, A. D. Mehta, E. Aksay, R. Stepnoski, and M. J. Schnitzer, J. Neurophysiol. 92, 3121 (2004).
[CrossRef] [PubMed]

Aksay, E.

J. C. Jung, A. D. Mehta, E. Aksay, R. Stepnoski, and M. J. Schnitzer, J. Neurophysiol. 92, 3121 (2004).
[CrossRef] [PubMed]

Celli, J. P.

W. Zhong, J. P. Celli, I. Rizvi, Z. Mai, B. Q. Spring, S. H. Yun, and T. Hasan, Br. J. Cancer 101, 2015 (2009).
[CrossRef] [PubMed]

Forbrich, A.

Hajireza, P.

W. Shi, P. Hajireza, P. Shao, A. Forbrich, and R. J. Zemp, Opt. Express 19, 17143 (2011).
[CrossRef] [PubMed]

P. Hajireza, W. Shi, P. Shao, S. Kerr, and R. J. Zemp, Proc. SPIE 7899, 78990 (2011).
[CrossRef]

Hasan, T.

W. Zhong, J. P. Celli, I. Rizvi, Z. Mai, B. Q. Spring, S. H. Yun, and T. Hasan, Br. J. Cancer 101, 2015 (2009).
[CrossRef] [PubMed]

Hu, S.

Jiao, S.

Jung, J. C.

J. C. Jung, A. D. Mehta, E. Aksay, R. Stepnoski, and M. J. Schnitzer, J. Neurophysiol. 92, 3121 (2004).
[CrossRef] [PubMed]

Kerr, S.

P. Hajireza, W. Shi, P. Shao, S. Kerr, and R. J. Zemp, Proc. SPIE 7899, 78990 (2011).
[CrossRef]

Li, L.

Liu, H.

X. Xu, H. Liu, and L. V. Wang, Nat. Photon. 5, 154 (2011).
[CrossRef]

Lorenser, D.

Mai, Z.

W. Zhong, J. P. Celli, I. Rizvi, Z. Mai, B. Q. Spring, S. H. Yun, and T. Hasan, Br. J. Cancer 101, 2015 (2009).
[CrossRef] [PubMed]

Manniesing, R.

R. Manniesing and W. Niessen, Inf. Process Med. Imaging 19, 138 (2005).
[CrossRef]

Maslov, K.

Mehta, A. D.

J. C. Jung, A. D. Mehta, E. Aksay, R. Stepnoski, and M. J. Schnitzer, J. Neurophysiol. 92, 3121 (2004).
[CrossRef] [PubMed]

Niessen, W.

R. Manniesing and W. Niessen, Inf. Process Med. Imaging 19, 138 (2005).
[CrossRef]

Puliafito, C. A.

Rajesh, S. P.

Rao, B.

Rizvi, I.

W. Zhong, J. P. Celli, I. Rizvi, Z. Mai, B. Q. Spring, S. H. Yun, and T. Hasan, Br. J. Cancer 101, 2015 (2009).
[CrossRef] [PubMed]

Sampson, D. D.

Schnitzer, M. J.

J. C. Jung, A. D. Mehta, E. Aksay, R. Stepnoski, and M. J. Schnitzer, J. Neurophysiol. 92, 3121 (2004).
[CrossRef] [PubMed]

Shao, P.

P. Hajireza, W. Shi, P. Shao, S. Kerr, and R. J. Zemp, Proc. SPIE 7899, 78990 (2011).
[CrossRef]

W. Shi, P. Hajireza, P. Shao, A. Forbrich, and R. J. Zemp, Opt. Express 19, 17143 (2011).
[CrossRef] [PubMed]

Shi, W.

W. Shi, P. Hajireza, P. Shao, A. Forbrich, and R. J. Zemp, Opt. Express 19, 17143 (2011).
[CrossRef] [PubMed]

P. Hajireza, W. Shi, P. Shao, S. Kerr, and R. J. Zemp, Proc. SPIE 7899, 78990 (2011).
[CrossRef]

Shung, K. K.

Spring, B. Q.

W. Zhong, J. P. Celli, I. Rizvi, Z. Mai, B. Q. Spring, S. H. Yun, and T. Hasan, Br. J. Cancer 101, 2015 (2009).
[CrossRef] [PubMed]

Stepnoski, R.

J. C. Jung, A. D. Mehta, E. Aksay, R. Stepnoski, and M. J. Schnitzer, J. Neurophysiol. 92, 3121 (2004).
[CrossRef] [PubMed]

Stoica, G.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Wang, L.

Wang, L. V.

Xie, Z.

Xu, X.

X. Xu, H. Liu, and L. V. Wang, Nat. Photon. 5, 154 (2011).
[CrossRef]

Yang, H.

Yang, J.

Yun, S. H.

W. Zhong, J. P. Celli, I. Rizvi, Z. Mai, B. Q. Spring, S. H. Yun, and T. Hasan, Br. J. Cancer 101, 2015 (2009).
[CrossRef] [PubMed]

Zemp, R. J.

W. Shi, P. Hajireza, P. Shao, A. Forbrich, and R. J. Zemp, Opt. Express 19, 17143 (2011).
[CrossRef] [PubMed]

P. Hajireza, W. Shi, P. Shao, S. Kerr, and R. J. Zemp, Proc. SPIE 7899, 78990 (2011).
[CrossRef]

Zhang, H. F.

Z. Xie, S. Jiao, H. F. Zhang, and C. A. Puliafito, Opt. Lett. 34, 1771 (2009).
[CrossRef] [PubMed]

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Zhong, W.

W. Zhong, J. P. Celli, I. Rizvi, Z. Mai, B. Q. Spring, S. H. Yun, and T. Hasan, Br. J. Cancer 101, 2015 (2009).
[CrossRef] [PubMed]

Zhou, Q.

Br. J. Cancer (1)

W. Zhong, J. P. Celli, I. Rizvi, Z. Mai, B. Q. Spring, S. H. Yun, and T. Hasan, Br. J. Cancer 101, 2015 (2009).
[CrossRef] [PubMed]

Inf. Process Med. Imaging (1)

R. Manniesing and W. Niessen, Inf. Process Med. Imaging 19, 138 (2005).
[CrossRef]

J. Neurophysiol. (1)

J. C. Jung, A. D. Mehta, E. Aksay, R. Stepnoski, and M. J. Schnitzer, J. Neurophysiol. 92, 3121 (2004).
[CrossRef] [PubMed]

Nat. Biotechnol. (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef] [PubMed]

Nat. Photon. (1)

X. Xu, H. Liu, and L. V. Wang, Nat. Photon. 5, 154 (2011).
[CrossRef]

Opt. Express (3)

Opt. Lett. (3)

Proc. SPIE (1)

P. Hajireza, W. Shi, P. Shao, S. Kerr, and R. J. Zemp, Proc. SPIE 7899, 78990 (2011).
[CrossRef]

Other (1)

Laser Institute of America, American National Standard for Safe Use of Lasers, ANSI Z136.1-2000 (American National Standards Institute, Inc., 2000).

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Figures (4)

Fig. 1
Fig. 1

Simplified scanning F-OR-PAM experimental setup. (FLD: fiber laser driver, Yb: Ytterbium, M: mirror, GS: glass, PD: photodiode, C1: first controller, C2: second controller, DX: X axis mirror driver, DY: Y axis mirror driver, OL: objective lens.

Fig. 2
Fig. 2

F-OR-PAM images demonstrating the entire field-of-view of the image-guide. In (a) the whole aperture is covered by black tape, (b) black tape moved to cover only half of the aperture.

Fig. 3
Fig. 3

(a) and (b) images of 7 μm carbon-fiber network. (c) Imaging of human hair with 100 μm diameter.

Fig. 4
Fig. 4

F-OR-PAM image of the microvasculature in a Swiss Webster mouse ear in vivo.

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