Abstract

We recently proposed photoacoustic correlation spectroscopy (PACS) and demonstrated a proof-of-concept experiment. Here we use the technique for in vivo flow speed measurement in capillaries in a chick embryo model. The photoacoustic microscopy system is used to render high spatial resolution and high sensitivity, enabling sufficient signals from single red blood cells. The probe beam size is calibrated by a blood-mimicking phantom. The results indicate the feasibility of using PACS to study flow speeds in capillaries.

© 2011 Optical Society of America

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References

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2011

P. Horn, M. M. Cortese-Krott, S. Keymel, I. Kumara, S. Burghoff, J. Schrader, M. Kelm, and P. Kleinbongard, Free Radic. Res. Commun. 45, 653 (2011).
[CrossRef]

J. Tam, P. Tiruveedhula, and A. Roorda, Biomed. Opt. Express 2, 781 (2011).
[CrossRef] [PubMed]

2010

2009

H. Fang and L. V. Wang, Opt. Lett. 34, 671 (2009).
[CrossRef] [PubMed]

Z. Xie, S. Jiao, H. F. Zhang, and C. A. Puliafito, Opt. Lett. 34, 1771 (2009).
[CrossRef] [PubMed]

V. M. Belichenko and K. A. Shoshenko, Russ. J. Dev. Biol. 40, 95 (2009).
[CrossRef]

J. A. Martin and A. Roorda, Exp. Eye Res. 88, 356 (2009).
[CrossRef]

2008

2007

P. Vennemann, R. Lindken, and J. Westerweel, Exp. Fluids 42, 495 (2007).
[CrossRef]

H. Fang, K. Maslov, and L. V. Wang, Phys. Rev. Lett. 99, 184501 (2007).
[CrossRef] [PubMed]

2004

E. Haustein and P. Schwille, Curr. Opin. Struct. Biol. 14, 531 (2004).
[CrossRef] [PubMed]

2000

R. H. Köhler, P. Schwille, W. W. Webb, and M. R. Hanson, J. Cell Sci. 113, 3921 (2000).
[PubMed]

1994

S. Wolf, O. Arend, K. Schulte, T. H. Ittel, and M. Reim, Hypertension 23, 464 (1994).
[PubMed]

1992

R. Baumann and H.-J. Meuer, Physiol. Rev. 72, 941 (1992).
[PubMed]

Arend, O.

S. Wolf, O. Arend, K. Schulte, T. H. Ittel, and M. Reim, Hypertension 23, 464 (1994).
[PubMed]

Baac, H. W.

Baker, J. R.

Baumann, R.

R. Baumann and H.-J. Meuer, Physiol. Rev. 72, 941 (1992).
[PubMed]

Belichenko, V. M.

V. M. Belichenko and K. A. Shoshenko, Russ. J. Dev. Biol. 40, 95 (2009).
[CrossRef]

Burghoff, S.

P. Horn, M. M. Cortese-Krott, S. Keymel, I. Kumara, S. Burghoff, J. Schrader, M. Kelm, and P. Kleinbongard, Free Radic. Res. Commun. 45, 653 (2011).
[CrossRef]

Butcher, J. T.

H. C. Yalcin, A. Shekhar, A. A. Rane, and J. T. Butcher, J. Visualized Exp. 44, doi:10.3791/2154 (2010).
[CrossRef]

Chang, Y.-C.

Chen, S.-L.

Chen, Y.

Cortese-Krott, M. M.

P. Horn, M. M. Cortese-Krott, S. Keymel, I. Kumara, S. Burghoff, J. Schrader, M. Kelm, and P. Kleinbongard, Free Radic. Res. Commun. 45, 653 (2011).
[CrossRef]

Fang, H.

H. Fang and L. V. Wang, Opt. Lett. 34, 671 (2009).
[CrossRef] [PubMed]

H. Fang, K. Maslov, and L. V. Wang, Phys. Rev. Lett. 99, 184501 (2007).
[CrossRef] [PubMed]

Guo, L. J.

Hanson, M. R.

R. H. Köhler, P. Schwille, W. W. Webb, and M. R. Hanson, J. Cell Sci. 113, 3921 (2000).
[PubMed]

Haustein, E.

E. Haustein and P. Schwille, Curr. Opin. Struct. Biol. 14, 531 (2004).
[CrossRef] [PubMed]

Horn, P.

P. Horn, M. M. Cortese-Krott, S. Keymel, I. Kumara, S. Burghoff, J. Schrader, M. Kelm, and P. Kleinbongard, Free Radic. Res. Commun. 45, 653 (2011).
[CrossRef]

Huang, S.-W.

Ittel, T. H.

S. Wolf, O. Arend, K. Schulte, T. H. Ittel, and M. Reim, Hypertension 23, 464 (1994).
[PubMed]

Jiao, S.

Kelm, M.

P. Horn, M. M. Cortese-Krott, S. Keymel, I. Kumara, S. Burghoff, J. Schrader, M. Kelm, and P. Kleinbongard, Free Radic. Res. Commun. 45, 653 (2011).
[CrossRef]

Keymel, S.

P. Horn, M. M. Cortese-Krott, S. Keymel, I. Kumara, S. Burghoff, J. Schrader, M. Kelm, and P. Kleinbongard, Free Radic. Res. Commun. 45, 653 (2011).
[CrossRef]

Kleinbongard, P.

P. Horn, M. M. Cortese-Krott, S. Keymel, I. Kumara, S. Burghoff, J. Schrader, M. Kelm, and P. Kleinbongard, Free Radic. Res. Commun. 45, 653 (2011).
[CrossRef]

Köhler, R. H.

R. H. Köhler, P. Schwille, W. W. Webb, and M. R. Hanson, J. Cell Sci. 113, 3921 (2000).
[PubMed]

Kumara, I.

P. Horn, M. M. Cortese-Krott, S. Keymel, I. Kumara, S. Burghoff, J. Schrader, M. Kelm, and P. Kleinbongard, Free Radic. Res. Commun. 45, 653 (2011).
[CrossRef]

Lindken, R.

P. Vennemann, R. Lindken, and J. Westerweel, Exp. Fluids 42, 495 (2007).
[CrossRef]

Ling, T.

Martin, J. A.

J. A. Martin and A. Roorda, Exp. Eye Res. 88, 356 (2009).
[CrossRef]

Maslov, K.

H. Fang, K. Maslov, and L. V. Wang, Phys. Rev. Lett. 99, 184501 (2007).
[CrossRef] [PubMed]

Maslov, K. I.

Meuer, H.-J.

R. Baumann and H.-J. Meuer, Physiol. Rev. 72, 941 (1992).
[PubMed]

Norris, T. B.

Puliafito, C. A.

Rane, A. A.

H. C. Yalcin, A. Shekhar, A. A. Rane, and J. T. Butcher, J. Visualized Exp. 44, doi:10.3791/2154 (2010).
[CrossRef]

Reim, M.

S. Wolf, O. Arend, K. Schulte, T. H. Ittel, and M. Reim, Hypertension 23, 464 (1994).
[PubMed]

Roorda, A.

Schrader, J.

P. Horn, M. M. Cortese-Krott, S. Keymel, I. Kumara, S. Burghoff, J. Schrader, M. Kelm, and P. Kleinbongard, Free Radic. Res. Commun. 45, 653 (2011).
[CrossRef]

Schulte, K.

S. Wolf, O. Arend, K. Schulte, T. H. Ittel, and M. Reim, Hypertension 23, 464 (1994).
[PubMed]

Schwille, P.

E. Haustein and P. Schwille, Curr. Opin. Struct. Biol. 14, 531 (2004).
[CrossRef] [PubMed]

R. H. Köhler, P. Schwille, W. W. Webb, and M. R. Hanson, J. Cell Sci. 113, 3921 (2000).
[PubMed]

Shekhar, A.

H. C. Yalcin, A. Shekhar, A. A. Rane, and J. T. Butcher, J. Visualized Exp. 44, doi:10.3791/2154 (2010).
[CrossRef]

Shi, Y.

Shoshenko, K. A.

V. M. Belichenko and K. A. Shoshenko, Russ. J. Dev. Biol. 40, 95 (2009).
[CrossRef]

Taber, L. A.

Tam, J.

Thomas, T.

Tiruveedhula, P.

Vennemann, P.

P. Vennemann, R. Lindken, and J. Westerweel, Exp. Fluids 42, 495 (2007).
[CrossRef]

Wang, L. V.

Webb, W. W.

R. H. Köhler, P. Schwille, W. W. Webb, and M. R. Hanson, J. Cell Sci. 113, 3921 (2000).
[PubMed]

Westerweel, J.

P. Vennemann, R. Lindken, and J. Westerweel, Exp. Fluids 42, 495 (2007).
[CrossRef]

Wolf, S.

S. Wolf, O. Arend, K. Schulte, T. H. Ittel, and M. Reim, Hypertension 23, 464 (1994).
[PubMed]

Xie, Z.

Yalcin, H. C.

H. C. Yalcin, A. Shekhar, A. A. Rane, and J. T. Butcher, J. Visualized Exp. 44, doi:10.3791/2154 (2010).
[CrossRef]

Yao, J.

Ye, J. Y.

Zhang, H. F.

Biomed. Opt. Express

Curr. Opin. Struct. Biol.

E. Haustein and P. Schwille, Curr. Opin. Struct. Biol. 14, 531 (2004).
[CrossRef] [PubMed]

Exp. Eye Res.

J. A. Martin and A. Roorda, Exp. Eye Res. 88, 356 (2009).
[CrossRef]

Exp. Fluids

P. Vennemann, R. Lindken, and J. Westerweel, Exp. Fluids 42, 495 (2007).
[CrossRef]

Free Radic. Res. Commun.

P. Horn, M. M. Cortese-Krott, S. Keymel, I. Kumara, S. Burghoff, J. Schrader, M. Kelm, and P. Kleinbongard, Free Radic. Res. Commun. 45, 653 (2011).
[CrossRef]

Hypertension

S. Wolf, O. Arend, K. Schulte, T. H. Ittel, and M. Reim, Hypertension 23, 464 (1994).
[PubMed]

J. Cell Sci.

R. H. Köhler, P. Schwille, W. W. Webb, and M. R. Hanson, J. Cell Sci. 113, 3921 (2000).
[PubMed]

J. Visualized Exp.

H. C. Yalcin, A. Shekhar, A. A. Rane, and J. T. Butcher, J. Visualized Exp. 44, doi:10.3791/2154 (2010).
[CrossRef]

Opt. Express

Opt. Lett.

Phys. Rev. Lett.

H. Fang, K. Maslov, and L. V. Wang, Phys. Rev. Lett. 99, 184501 (2007).
[CrossRef] [PubMed]

Physiol. Rev.

R. Baumann and H.-J. Meuer, Physiol. Rev. 72, 941 (1992).
[PubMed]

Russ. J. Dev. Biol.

V. M. Belichenko and K. A. Shoshenko, Russ. J. Dev. Biol. 40, 95 (2009).
[CrossRef]

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Figures (3)

Fig. 1
Fig. 1

Schematic of PACS flow speed measurement using the PAM system.

Fig. 2
Fig. 2

(a) Dependence of the flow time on the design flow speed. The square symbols and the error bars represent the mean flow time and the standard deviations, respectively. The solid line is the curve fitting. (b) (Top) A-line signals, (middle) its Hilbert transform displayed over a 50 dB dynamic range for better contrast, and (bottom) the P ( t ) . Only the first 0.5 s are plotted. (c) Calculated and fitted autocorrelation curves in dotted and solid lines, respectively.

Fig. 3
Fig. 3

(a) Photograph of an 8 d old chick embryo. (b) Maximum amplitude projection image on the x y plane of the chick embryo. Red crosses, three different positions for flow speed evaluation. (c) (Top) A-line signals, (middle) its Hilbert transform displayed over a 25 dB dynamic range, and (bottom) the P ( t ) . (d) Calculated and fitted autocorrela tion curves in dotted and solid lines, respectively. The fitted τ f = 99 ms .

Equations (1)

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G ( τ ) = 1 / N × exp ( τ / τ f ) 2 ,

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