Abstract

The condenser is one of the main components in most transmitted light compound microscopes. In this Letter, we show that such a condenser can be replaced by a programmable LED array to achieve greater imaging flexibility and functionality. Without mechanically scanning the sample or changing the microscope setup, the proposed approach can be used for dark-field imaging, bright-field imaging, microscopy sectioning, and digital refocusing. Images of a starfish embryo were acquired by using such an approach for demonstration.

© 2011 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Microscopy illumination engineering using a low-cost liquid crystal display

Kaikai Guo, Zichao Bian, Siyuan Dong, Pariksheet Nanda, Ying Min Wang, and Guoan Zheng
Biomed. Opt. Express 6(2) 574-579 (2015)

Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging

Donghak Lee, Suho Ryu, Uihan Kim, Daeseong Jung, and Chulmin Joo
Biomed. Opt. Express 6(12) 4912-4922 (2015)

Diffraction tomography with Fourier ptychography

Roarke Horstmeyer, Jaebum Chung, Xiaoze Ou, Guoan Zheng, and Changhuei Yang
Optica 3(8) 827-835 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription