We developed a photoacoustic imaging system that has real-time imaging capability with optical resolution. The imaging system is capable of scanning at over a range and up to over a scanning range. A focused laser beam provides a lateral resolution of as measured in an optically nonscattering medium. Flows of micrometer-sized carbon particles or whole blood in a silicone tube and individual red blood cells (RBCs) in mouse ear capillaries were also imaged in real time, demonstrating the capability to image highly dynamic processes in vivo at a micrometer-scale resolution.
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