Abstract

Conventional fluorescence detection is sensitive to an intricate product of the number of fluorescent emitters times the local excitation intensity. Here, we describe a method to locally quantify the excitation intensity and the number of emitters separately, enabling a clear distinction between the phenomena responsible for a given fluorescence signal. Our technique is based on harmonic excitation modulation and higher-order fluorescence demodulation. It is compatible with a wide range of observations, opening new characterization routes for fluorescence detection or imaging on complex systems, such as plasmonic substrates or scattering media.

© 2011 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Saturated excitation of fluorescence to quantify excitation enhancement in aperture antennas

Heykel Aouani, Richard Hostein, Oussama Mahboub, Eloïse Devaux, Hervé Rigneault, Thomas W. Ebbesen, and Jérôme Wenger
Opt. Express 20(16) 18085-18090 (2012)

Revealing single emitter spectral dynamics from intensity correlations in an ensemble fluorescence spectrum

Xavier Brokmann, Lisa Marshall, and Moungi Bawendi
Opt. Express 17(6) 4509-4517 (2009)

Frequency dependent detection in a STED microscope using modulated excitation light

Emiliano Ronzitti, Benjamin Harke, and Alberto Diaspro
Opt. Express 21(1) 210-219 (2013)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (2)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (7)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription