Coherent Raman scattering methods allow for label-free imaging of tissue with chemical contrast and high spatial and temporal resolution. However, their imaging depth in scattering tissue is limited to less than , requiring the development of endoscopes to obtain images deep inside the body. Here, we describe a coherent Raman endoscope that provides stimulated Raman scattering images at seven frames per second using a miniaturized fiber scanner, a custom-designed objective lens, and an optimized scheme for collection of scattered light from the tissue. We characterize the system and demonstrate chemical selectivity in mouse tissue images.
© 2011 Optical Society of AmericaFull Article | PDF Article
François Légaré, Conor L. Evans, Feruz Ganikhanov, and X. Sunney Xie
Opt. Express 14(10) 4427-4432 (2006)
Mihaela Balu, Gangjun Liu, Zhongping Chen, Bruce J. Tromberg, and Eric O. Potma
Opt. Express 18(3) 2380-2388 (2010)
Sangeeta Murugkar, Brett Smith, Prateek Srivastava, Adrian Moica, Majid Naji, Craig Brideau, Peter K. Stys, and Hanan Anis
Opt. Express 18(23) 23796-23804 (2010)