Abstract

We have developed a focal plane tuning technique for use in focus-grid-based wide-field-of-view microscopy (WFM). In WFM, the incidence of a collimated beam on a mask with a two-dimensional grid of aperture produced the Talbot images of the aperture grid. The Talbot pattern functioned as a focus grid and was used to illuminate the sample. By scanning the sample across the focus grid and collecting the transmission, we can generate a microscopy image of the sample. By tuning the wavelength of the laser, we can tune the focal plane of the WFM and acquire images of different depth into the sample. Images of a green algae microscope slide were acquired at different focal planes for demonstration.

© 2011 Optical Society of America

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References

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2010 (2)

2007 (1)

M. Oheim, Br. J. Pharmacol. 152, 1 (2007).
[CrossRef] [PubMed]

2006 (2)

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

M. G. Rojo, G. B. Garcia, C. P. Mateos, J. G. Garcia, and M. C. Vicente, Int. J. Surg. Pathol. 14, 285 (2006).
[CrossRef] [PubMed]

2005 (1)

R. Graf, J. Rietdorf, and T. Zimmermann, Adv. Biochem. Eng. Biotechnol. 95, 1311 (2005).

2004 (1)

1997 (1)

B. Besold and N. Lindlein, Opt. Eng. 36, 1099 (1997).
[CrossRef]

1990 (1)

1989 (1)

K. Patorski, Prog. Opt. 27, 1 (1989).
[CrossRef]

1836 (1)

H. F. Talbot, Philos. Mag. 9, 401 (1836).

Anthony, L.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Besold, B.

B. Besold and N. Lindlein, Opt. Eng. 36, 1099 (1997).
[CrossRef]

Bishara, W.

Coskun, A. F.

Cui, X.

di Mambro, E.

Garcia, G. B.

M. G. Rojo, G. B. Garcia, C. P. Mateos, J. G. Garcia, and M. C. Vicente, Int. J. Surg. Pathol. 14, 285 (2006).
[CrossRef] [PubMed]

Garcia, J. G.

M. G. Rojo, G. B. Garcia, C. P. Mateos, J. G. Garcia, and M. C. Vicente, Int. J. Surg. Pathol. 14, 285 (2006).
[CrossRef] [PubMed]

Gilbertson, J. R.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Graf, R.

R. Graf, J. Rietdorf, and T. Zimmermann, Adv. Biochem. Eng. Biotechnol. 95, 1311 (2005).

Guerineau, N.

Haidar, R.

Ho, J.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Jukic, D. M.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Lee, L. M.

Leger, J. R.

Lindlein, N.

B. Besold and N. Lindlein, Opt. Eng. 36, 1099 (1997).
[CrossRef]

Mateos, C. P.

M. G. Rojo, G. B. Garcia, C. P. Mateos, J. G. Garcia, and M. C. Vicente, Int. J. Surg. Pathol. 14, 285 (2006).
[CrossRef] [PubMed]

Oheim, M.

M. Oheim, Br. J. Pharmacol. 152, 1 (2007).
[CrossRef] [PubMed]

Ozcan, A.

Parwani, A. V.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Patorski, K.

K. Patorski, Prog. Opt. 27, 1 (1989).
[CrossRef]

Primot, J.

Rietdorf, J.

R. Graf, J. Rietdorf, and T. Zimmermann, Adv. Biochem. Eng. Biotechnol. 95, 1311 (2005).

Rojo, M. G.

M. G. Rojo, G. B. Garcia, C. P. Mateos, J. G. Garcia, and M. C. Vicente, Int. J. Surg. Pathol. 14, 285 (2006).
[CrossRef] [PubMed]

Su, T. W.

Swanson, G. J.

Talbot, H. F.

H. F. Talbot, Philos. Mag. 9, 401 (1836).

Vicente, M. C.

M. G. Rojo, G. B. Garcia, C. P. Mateos, J. G. Garcia, and M. C. Vicente, Int. J. Surg. Pathol. 14, 285 (2006).
[CrossRef] [PubMed]

Wang, Y. M.

Wu, J.

Yagi, Y.

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Yang, C.

Zheng, G.

Zimmermann, T.

R. Graf, J. Rietdorf, and T. Zimmermann, Adv. Biochem. Eng. Biotechnol. 95, 1311 (2005).

Adv. Biochem. Eng. Biotechnol. (1)

R. Graf, J. Rietdorf, and T. Zimmermann, Adv. Biochem. Eng. Biotechnol. 95, 1311 (2005).

Br. J. Pharmacol. (1)

M. Oheim, Br. J. Pharmacol. 152, 1 (2007).
[CrossRef] [PubMed]

Hum. Pathol. (1)

J. Ho, A. V. Parwani, D. M. Jukic, Y. Yagi, L. Anthony, and J. R. Gilbertson, Hum. Pathol. 37, 322 (2006).
[CrossRef] [PubMed]

Int. J. Surg. Pathol. (1)

M. G. Rojo, G. B. Garcia, C. P. Mateos, J. G. Garcia, and M. C. Vicente, Int. J. Surg. Pathol. 14, 285 (2006).
[CrossRef] [PubMed]

J. Opt. Soc. Am. A (1)

Opt. Eng. (1)

B. Besold and N. Lindlein, Opt. Eng. 36, 1099 (1997).
[CrossRef]

Opt. Express (1)

Opt. Lett. (2)

Philos. Mag. (1)

H. F. Talbot, Philos. Mag. 9, 401 (1836).

Prog. Opt. (1)

K. Patorski, Prog. Opt. 27, 1 (1989).
[CrossRef]

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Figures (3)

Fig. 1
Fig. 1

Evolving of Talbot patterns around Z = Z 1.5 . (a) Simulation spots for λ 1 = 702 nm . (b) Spots for λ 1 = 702 nm . (c) Spots for λ 2 = 692 nm . w is the FWHM spot size, and Z 1.5 , λ 2 Z 1.5 , λ 1 = 55 μm . The size of each pattern figure is 30 μm × 30 μm .

Fig. 2
Fig. 2

(a) Experimental setup of the wide-FOV microscope based on the Talbot illumination. (b) Part of the aperture grid on the mask plane. (c) Part of the focus grid at Z = Z 15 . The number of spots along the x and y directions for the full mask is indicated.

Fig. 3
Fig. 3

Images acquired by the Talbot wide-FOV microscope with an effective FOV of 6.4 mm × 4.2 mm . (a) Wide-FOV image of a USAF target, with effective FOV indicated in the large dashed rectangle. (b) Expanded view of the smallest feature of the target and cross-sectional profile of group 8, element 6. (c) Wide-FOV image of a mixed green algae slide with λ = 692 nm , with effective FOV indicated in the large dashed rectangle. (d) Expanded view of the region indicated in (c). (e) Wide-FOV image of a mixed green algae slide with λ = 702 nm , with effective FOV indicated in the large dashed rectangle. (f) Expanded view of the region indicated in (e).

Equations (2)

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Z T = 2 d 2 / λ ,
Δ Z T = 2 d 2 Δ λ / λ 2 .

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