Abstract

Using a single-beam, oscillating optical tweezers, we demonstrate trapping and rotation of rod-shaped bacterial cells with respect to the optical axis. The angle of rotation, θ, is determined by the amplitude of the oscillation. It is shown that θ can be measured from the longitudinal cell intensity profiles in the corresponding phase-contrast images. The technique allows viewing the cell from different perspectives and can provide a useful tool in fluorescence microscopy for the analysis of three-dimensional subcellular structures.

© 2011 Optical Society of America

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2008

E. Itan, G. Carmon, A. Rabinovitch, I. Fishov, and M. Feingold, Phys. Rev. E 77, 061902 (2008).
[CrossRef]

G. Reshes, S. Vanounou, I. Fishov, and M. Feingold, Biophys. J. 94, 251 (2008).
[CrossRef]

2003

V. Bingelyte, J. Leach, J. Courtial, and M. J. Padgett, Appl. Phys. Lett. 82, 829 (2003).
[CrossRef]

2002

2001

L. Paterson, M. P. MacDonald, J. Arlt, W. Sibbett, P. E. Bryant, and K. Dholakia, Science 292, 912 (2001).
[CrossRef] [PubMed]

E. Higurashi, R. Sawada, and T. Ito, J. Micromech. Microeng. 11, 140 (2001).
[CrossRef]

D. Velegol and F. Lanni, Biophys. J. 81, 1786 (2001).
[CrossRef] [PubMed]

1999

S. Juodkazis, M. Shikata, T. Takahashi, S. Matsuo, and H. Misawa, Appl. Phys. Lett. 74, 3627 (1999).
[CrossRef]

E. Higurashi, R. Sawada, and T. Ito, Phys. Rev. E 59, 3676 (1999).
[CrossRef]

X. Yao, M. Jericho, D. Pink, and T. Beveridge, J. Bacteriol. 181, 6865 (1999).
[PubMed]

1998

E. Higurashi, R. Sawada, and T. Ito, Appl. Phys. Lett. 73, 3034 (1998).
[CrossRef]

M. E. J. Friese, T. A. Neiminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Nature 394, 348 (1998).
[CrossRef]

1997

1996

M. E. J. Friese, J. Enger, H. Rubinsztein-Dunlop, and N. R. Heckenberg, Phys. Rev. A 54, 1593 (1996).
[CrossRef] [PubMed]

1995

H. He, M. E. J. Friese, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Phys. Rev. Lett. 75, 826 (1995).
[CrossRef] [PubMed]

1994

E. Higurashi, H. Ukita, H. Tanaka, and O. Ohguchi, Appl. Phys. Lett. 64, 2209 (1994).
[CrossRef]

M. Gu and C. J. R. Sheppard, Appl. Opt. 33, 625(1994).
[CrossRef] [PubMed]

1993

K. Visscher, G. J. Brakenhoff, and J. J. Krol, Cytometry 14, 105 (1993).
[CrossRef] [PubMed]

1992

H. Misawa, K. Sasaki, M. Koshioka, N. Kitamura, and H. Masuhara, Appl. Phys. Lett. 60, 310 (1992).
[CrossRef]

1991

D. A. Schafer, J. Gelles, M. P. Sheetz, and R. Landick, Nature 352, 444 (1991).
[CrossRef] [PubMed]

A. Glindemann and J. Kross, J. Mod. Opt. 38, 379(1991).
[CrossRef]

S. Sato, M. Ishigure, and H. Inaba, Electron. Lett. 27, 1831 (1991).
[CrossRef]

1987

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef] [PubMed]

1986

Allen, L.

Arlt, J.

L. Paterson, M. P. MacDonald, J. Arlt, W. Sibbett, P. E. Bryant, and K. Dholakia, Science 292, 912 (2001).
[CrossRef] [PubMed]

Ashkin, A.

Axner, O.

Babcock, H.

S. R. Quake, H. Babcock, and S. Chu, Nature 388, 151 (1997).
[CrossRef] [PubMed]

Beveridge, T.

X. Yao, M. Jericho, D. Pink, and T. Beveridge, J. Bacteriol. 181, 6865 (1999).
[PubMed]

Bingelyte, V.

V. Bingelyte, J. Leach, J. Courtial, and M. J. Padgett, Appl. Phys. Lett. 82, 829 (2003).
[CrossRef]

Bjorkholm, J. E.

Brakenhoff, G. J.

K. Visscher, G. J. Brakenhoff, and J. J. Krol, Cytometry 14, 105 (1993).
[CrossRef] [PubMed]

Bryant, P. E.

L. Paterson, M. P. MacDonald, J. Arlt, W. Sibbett, P. E. Bryant, and K. Dholakia, Science 292, 912 (2001).
[CrossRef] [PubMed]

Carmon, G.

E. Itan, G. Carmon, A. Rabinovitch, I. Fishov, and M. Feingold, Phys. Rev. E 77, 061902 (2008).
[CrossRef]

Chu, S.

Courtial, J.

V. Bingelyte, J. Leach, J. Courtial, and M. J. Padgett, Appl. Phys. Lett. 82, 829 (2003).
[CrossRef]

Dholakia, K.

L. Paterson, M. P. MacDonald, J. Arlt, W. Sibbett, P. E. Bryant, and K. Dholakia, Science 292, 912 (2001).
[CrossRef] [PubMed]

N. B. Simpson, K. Dholakia, L. Allen, and M. J. Padgett, Opt. Lett. 22, 52 (1997).
[CrossRef] [PubMed]

Dziedzic, J. M.

Enger, J.

M. E. J. Friese, J. Enger, H. Rubinsztein-Dunlop, and N. R. Heckenberg, Phys. Rev. A 54, 1593 (1996).
[CrossRef] [PubMed]

Fällman, E.

Feingold, M.

G. Reshes, S. Vanounou, I. Fishov, and M. Feingold, Biophys. J. 94, 251 (2008).
[CrossRef]

E. Itan, G. Carmon, A. Rabinovitch, I. Fishov, and M. Feingold, Phys. Rev. E 77, 061902 (2008).
[CrossRef]

Fishov, I.

E. Itan, G. Carmon, A. Rabinovitch, I. Fishov, and M. Feingold, Phys. Rev. E 77, 061902 (2008).
[CrossRef]

G. Reshes, S. Vanounou, I. Fishov, and M. Feingold, Biophys. J. 94, 251 (2008).
[CrossRef]

Friese, M. E. J.

M. E. J. Friese, T. A. Neiminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Nature 394, 348 (1998).
[CrossRef]

M. E. J. Friese, J. Enger, H. Rubinsztein-Dunlop, and N. R. Heckenberg, Phys. Rev. A 54, 1593 (1996).
[CrossRef] [PubMed]

H. He, M. E. J. Friese, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Phys. Rev. Lett. 75, 826 (1995).
[CrossRef] [PubMed]

Gelles, J.

D. A. Schafer, J. Gelles, M. P. Sheetz, and R. Landick, Nature 352, 444 (1991).
[CrossRef] [PubMed]

Glindemann, A.

A. Glindemann and J. Kross, J. Mod. Opt. 38, 379(1991).
[CrossRef]

Gu, M.

He, H.

H. He, M. E. J. Friese, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Phys. Rev. Lett. 75, 826 (1995).
[CrossRef] [PubMed]

Heckenberg, N. R.

M. E. J. Friese, T. A. Neiminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Nature 394, 348 (1998).
[CrossRef]

M. E. J. Friese, J. Enger, H. Rubinsztein-Dunlop, and N. R. Heckenberg, Phys. Rev. A 54, 1593 (1996).
[CrossRef] [PubMed]

H. He, M. E. J. Friese, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Phys. Rev. Lett. 75, 826 (1995).
[CrossRef] [PubMed]

Higurashi, E.

E. Higurashi, R. Sawada, and T. Ito, J. Micromech. Microeng. 11, 140 (2001).
[CrossRef]

E. Higurashi, R. Sawada, and T. Ito, Phys. Rev. E 59, 3676 (1999).
[CrossRef]

E. Higurashi, R. Sawada, and T. Ito, Appl. Phys. Lett. 73, 3034 (1998).
[CrossRef]

E. Higurashi, H. Ukita, H. Tanaka, and O. Ohguchi, Appl. Phys. Lett. 64, 2209 (1994).
[CrossRef]

Inaba, H.

S. Sato, M. Ishigure, and H. Inaba, Electron. Lett. 27, 1831 (1991).
[CrossRef]

Ishigure, M.

S. Sato, M. Ishigure, and H. Inaba, Electron. Lett. 27, 1831 (1991).
[CrossRef]

Itan, E.

E. Itan, G. Carmon, A. Rabinovitch, I. Fishov, and M. Feingold, Phys. Rev. E 77, 061902 (2008).
[CrossRef]

Ito, T.

E. Higurashi, R. Sawada, and T. Ito, J. Micromech. Microeng. 11, 140 (2001).
[CrossRef]

E. Higurashi, R. Sawada, and T. Ito, Phys. Rev. E 59, 3676 (1999).
[CrossRef]

E. Higurashi, R. Sawada, and T. Ito, Appl. Phys. Lett. 73, 3034 (1998).
[CrossRef]

Jericho, M.

X. Yao, M. Jericho, D. Pink, and T. Beveridge, J. Bacteriol. 181, 6865 (1999).
[PubMed]

Juodkazis, S.

S. Juodkazis, M. Shikata, T. Takahashi, S. Matsuo, and H. Misawa, Appl. Phys. Lett. 74, 3627 (1999).
[CrossRef]

Kitamura, N.

H. Misawa, K. Sasaki, M. Koshioka, N. Kitamura, and H. Masuhara, Appl. Phys. Lett. 60, 310 (1992).
[CrossRef]

Koshioka, M.

H. Misawa, K. Sasaki, M. Koshioka, N. Kitamura, and H. Masuhara, Appl. Phys. Lett. 60, 310 (1992).
[CrossRef]

Krol, J. J.

K. Visscher, G. J. Brakenhoff, and J. J. Krol, Cytometry 14, 105 (1993).
[CrossRef] [PubMed]

Kross, J.

A. Glindemann and J. Kross, J. Mod. Opt. 38, 379(1991).
[CrossRef]

Landick, R.

D. A. Schafer, J. Gelles, M. P. Sheetz, and R. Landick, Nature 352, 444 (1991).
[CrossRef] [PubMed]

Lanni, F.

D. Velegol and F. Lanni, Biophys. J. 81, 1786 (2001).
[CrossRef] [PubMed]

Leach, J.

V. Bingelyte, J. Leach, J. Courtial, and M. J. Padgett, Appl. Phys. Lett. 82, 829 (2003).
[CrossRef]

MacDonald, M. P.

L. Paterson, M. P. MacDonald, J. Arlt, W. Sibbett, P. E. Bryant, and K. Dholakia, Science 292, 912 (2001).
[CrossRef] [PubMed]

Masuhara, H.

H. Misawa, K. Sasaki, M. Koshioka, N. Kitamura, and H. Masuhara, Appl. Phys. Lett. 60, 310 (1992).
[CrossRef]

Matsuo, S.

S. Juodkazis, M. Shikata, T. Takahashi, S. Matsuo, and H. Misawa, Appl. Phys. Lett. 74, 3627 (1999).
[CrossRef]

Misawa, H.

S. Juodkazis, M. Shikata, T. Takahashi, S. Matsuo, and H. Misawa, Appl. Phys. Lett. 74, 3627 (1999).
[CrossRef]

H. Misawa, K. Sasaki, M. Koshioka, N. Kitamura, and H. Masuhara, Appl. Phys. Lett. 60, 310 (1992).
[CrossRef]

Neiminen, T. A.

M. E. J. Friese, T. A. Neiminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Nature 394, 348 (1998).
[CrossRef]

O’Neil, A. T.

Ohguchi, O.

E. Higurashi, H. Ukita, H. Tanaka, and O. Ohguchi, Appl. Phys. Lett. 64, 2209 (1994).
[CrossRef]

Padgett, M. J.

Paterson, L.

L. Paterson, M. P. MacDonald, J. Arlt, W. Sibbett, P. E. Bryant, and K. Dholakia, Science 292, 912 (2001).
[CrossRef] [PubMed]

Pink, D.

X. Yao, M. Jericho, D. Pink, and T. Beveridge, J. Bacteriol. 181, 6865 (1999).
[PubMed]

Quake, S. R.

S. R. Quake, H. Babcock, and S. Chu, Nature 388, 151 (1997).
[CrossRef] [PubMed]

Rabinovitch, A.

E. Itan, G. Carmon, A. Rabinovitch, I. Fishov, and M. Feingold, Phys. Rev. E 77, 061902 (2008).
[CrossRef]

Reshes, G.

G. Reshes, S. Vanounou, I. Fishov, and M. Feingold, Biophys. J. 94, 251 (2008).
[CrossRef]

Rubinsztein-Dunlop, H.

M. E. J. Friese, T. A. Neiminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Nature 394, 348 (1998).
[CrossRef]

M. E. J. Friese, J. Enger, H. Rubinsztein-Dunlop, and N. R. Heckenberg, Phys. Rev. A 54, 1593 (1996).
[CrossRef] [PubMed]

H. He, M. E. J. Friese, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Phys. Rev. Lett. 75, 826 (1995).
[CrossRef] [PubMed]

Sasaki, K.

H. Misawa, K. Sasaki, M. Koshioka, N. Kitamura, and H. Masuhara, Appl. Phys. Lett. 60, 310 (1992).
[CrossRef]

Sato, S.

S. Sato, M. Ishigure, and H. Inaba, Electron. Lett. 27, 1831 (1991).
[CrossRef]

Sawada, R.

E. Higurashi, R. Sawada, and T. Ito, J. Micromech. Microeng. 11, 140 (2001).
[CrossRef]

E. Higurashi, R. Sawada, and T. Ito, Phys. Rev. E 59, 3676 (1999).
[CrossRef]

E. Higurashi, R. Sawada, and T. Ito, Appl. Phys. Lett. 73, 3034 (1998).
[CrossRef]

Schafer, D. A.

D. A. Schafer, J. Gelles, M. P. Sheetz, and R. Landick, Nature 352, 444 (1991).
[CrossRef] [PubMed]

Sheetz, M. P.

D. A. Schafer, J. Gelles, M. P. Sheetz, and R. Landick, Nature 352, 444 (1991).
[CrossRef] [PubMed]

Sheppard, C. J. R.

Shikata, M.

S. Juodkazis, M. Shikata, T. Takahashi, S. Matsuo, and H. Misawa, Appl. Phys. Lett. 74, 3627 (1999).
[CrossRef]

Sibbett, W.

L. Paterson, M. P. MacDonald, J. Arlt, W. Sibbett, P. E. Bryant, and K. Dholakia, Science 292, 912 (2001).
[CrossRef] [PubMed]

Simpson, N. B.

Takahashi, T.

S. Juodkazis, M. Shikata, T. Takahashi, S. Matsuo, and H. Misawa, Appl. Phys. Lett. 74, 3627 (1999).
[CrossRef]

Tanaka, H.

E. Higurashi, H. Ukita, H. Tanaka, and O. Ohguchi, Appl. Phys. Lett. 64, 2209 (1994).
[CrossRef]

Ukita, H.

E. Higurashi, H. Ukita, H. Tanaka, and O. Ohguchi, Appl. Phys. Lett. 64, 2209 (1994).
[CrossRef]

Vanounou, S.

G. Reshes, S. Vanounou, I. Fishov, and M. Feingold, Biophys. J. 94, 251 (2008).
[CrossRef]

Velegol, D.

D. Velegol and F. Lanni, Biophys. J. 81, 1786 (2001).
[CrossRef] [PubMed]

Visscher, K.

K. Visscher, G. J. Brakenhoff, and J. J. Krol, Cytometry 14, 105 (1993).
[CrossRef] [PubMed]

Yamane, T.

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef] [PubMed]

Yao, X.

X. Yao, M. Jericho, D. Pink, and T. Beveridge, J. Bacteriol. 181, 6865 (1999).
[PubMed]

Appl. Opt.

Appl. Phys. Lett.

H. Misawa, K. Sasaki, M. Koshioka, N. Kitamura, and H. Masuhara, Appl. Phys. Lett. 60, 310 (1992).
[CrossRef]

E. Higurashi, R. Sawada, and T. Ito, Appl. Phys. Lett. 73, 3034 (1998).
[CrossRef]

S. Juodkazis, M. Shikata, T. Takahashi, S. Matsuo, and H. Misawa, Appl. Phys. Lett. 74, 3627 (1999).
[CrossRef]

E. Higurashi, H. Ukita, H. Tanaka, and O. Ohguchi, Appl. Phys. Lett. 64, 2209 (1994).
[CrossRef]

V. Bingelyte, J. Leach, J. Courtial, and M. J. Padgett, Appl. Phys. Lett. 82, 829 (2003).
[CrossRef]

Biophys. J.

D. Velegol and F. Lanni, Biophys. J. 81, 1786 (2001).
[CrossRef] [PubMed]

G. Reshes, S. Vanounou, I. Fishov, and M. Feingold, Biophys. J. 94, 251 (2008).
[CrossRef]

Cytometry

K. Visscher, G. J. Brakenhoff, and J. J. Krol, Cytometry 14, 105 (1993).
[CrossRef] [PubMed]

Electron. Lett.

S. Sato, M. Ishigure, and H. Inaba, Electron. Lett. 27, 1831 (1991).
[CrossRef]

J. Bacteriol.

X. Yao, M. Jericho, D. Pink, and T. Beveridge, J. Bacteriol. 181, 6865 (1999).
[PubMed]

J. Micromech. Microeng.

E. Higurashi, R. Sawada, and T. Ito, J. Micromech. Microeng. 11, 140 (2001).
[CrossRef]

J. Mod. Opt.

A. Glindemann and J. Kross, J. Mod. Opt. 38, 379(1991).
[CrossRef]

Nature

M. E. J. Friese, T. A. Neiminen, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Nature 394, 348 (1998).
[CrossRef]

S. R. Quake, H. Babcock, and S. Chu, Nature 388, 151 (1997).
[CrossRef] [PubMed]

D. A. Schafer, J. Gelles, M. P. Sheetz, and R. Landick, Nature 352, 444 (1991).
[CrossRef] [PubMed]

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef] [PubMed]

Opt. Lett.

Phys. Rev. A

M. E. J. Friese, J. Enger, H. Rubinsztein-Dunlop, and N. R. Heckenberg, Phys. Rev. A 54, 1593 (1996).
[CrossRef] [PubMed]

Phys. Rev. E

E. Higurashi, R. Sawada, and T. Ito, Phys. Rev. E 59, 3676 (1999).
[CrossRef]

E. Itan, G. Carmon, A. Rabinovitch, I. Fishov, and M. Feingold, Phys. Rev. E 77, 061902 (2008).
[CrossRef]

Phys. Rev. Lett.

H. He, M. E. J. Friese, N. R. Heckenberg, and H. Rubinsztein-Dunlop, Phys. Rev. Lett. 75, 826 (1995).
[CrossRef] [PubMed]

Science

L. Paterson, M. P. MacDonald, J. Arlt, W. Sibbett, P. E. Bryant, and K. Dholakia, Science 292, 912 (2001).
[CrossRef] [PubMed]

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Figures (3)

Fig. 1
Fig. 1

(a) Experimental setup: M 1 to M 4 , mirrors ( M 3 is a dichroic mirror); L 1 , L 2 , lenses (telescope). (b) Schematic view of a rotated cell.

Fig. 2
Fig. 2

A large step θ scan of an E. coli cell. Angles 30 ° , 45 ° , 60 ° , 75 ° , and 90 ° were measured using the critical point method, 15 ° is obtained via the interpolation approach, and 0 ° corresponds to the case of a nonoscillating trap. Bar = 1 μm .

Fig. 3
Fig. 3

Testing the critical point method. For E. coli cells that were attached to the glass bottom of the sample in different orientations, we compare θ cp with θ g (squares). The θ g = θ cp line is also shown.

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